Diverzitet i sezonska distribucija mikromiceta u vazduhu prostorija za konzervaciju objekata kulturne baštine
Diversity and seasonal distribution of micromycetes in the air of cultural heritage conservation premises
Докторанд
Savković, ŽeljkoМентор
Ljaljević-Grbić, MilicaЧланови комисије
Vukojević, JelenaIvanović, Žarko
Džamić, Ana
Stupar, Miloš
Метаподаци
Приказ свих података о дисертацијиСажетак
Sprovedeno je istraživanje diverziteta mikromiceta i monitoring koncentracije
vijabilnih fungalnih propagula u vazduhu 20 odabranih prostorija za konzervaciju predmeta
kulturne baštine i vazduha neposredne okoline, tokom 4 sezone 2014/2015.
Dokumentovane su visoke vrednosti koncentracije spora u vazduhu tokom celog perioda
uzorkovanja, posebno tokom prolećnog i letnjeg perioda u prostorijama sa visokim RH
vrednostima. U uzorcima vazduha identifikovano je ukupno 74 vrste gljiva a najbrojnije su
bile Aspergillus spp. i Penicillium spp. Na osnovu dobijenih vrednosti relativne gustine i
relativne učestalosti, najzastupljeniji aerogeni izolati iz ispitivanih prostorija su bili
pripadnici Cladosporium i Penicillium vrsta. Na osnovu in vitro testova biodeterioracije
aerogenih izolata Aspergillus, Penicillium i Talaromyces vrsta pokazana je sposobnost
produkcije ekstracelularnih enzima, kiselina i pigmenata, formiranja mikogenih minerala i
hemolitička aktivnost. Takođe, urađena je mikološka an...aliza kamene stele koja je bila na
konzervacijskom tretmanu i zabeležen je visok nivo kolonizacije i deterioracije kamenog
suspstrata što odgovara dobijenoj vrednosti indeksa deterioracije. Dokumentovana je
sposobnost produkcije kiselih i baznih metabolita, produkcije pigmenata, solubilizacija
krečnjaka i formiranje mikogenih minerala autohtonih izolata sa stele. Na kraju, analiziran
je antifungalni potencijal odabranih etarskih ulja, metil salicilata i benzalkonijum hlorida na
aerogenim izolatima Aspergillus spp. i Penicillium spp. a najjača antifungalna aktivnost je
zabeležena za benzalkonijum hlorid i etarsko ulje Cinnamomum zeylanicum. Rezultati
molekularnog dokinga dominantnih komponenti etarskih ulja su bili u korelaciji sa jačinom
antifungalne aktivnosti ispitivanih biocida.
Diversity and monitoring of viable fungal propagules was investigated in the air of
20 selected cultural heritage conservation premises as well as in the outdoor air during 4
seasons of the 2014/2015 period. High spore loads were determined in the air during the
study period, especially during spring and summer in the rooms with high RH values. A
total of 74 fungal species were documented while most numerous species were Aspergillus
spp. (19) and Penicillium spp. (15). According to obtained relative frequency and relative
density values, the most frequent airborne isolates were Cladosporium spp. and Penicillium
spp. In vitro biodeterioration tests were applied on airborne Aspergillus, Penicillium and
Talaromyces isolates and production of extracellular enzymes, acids and pigments was
demonstrated as well as the ability to form mycogenic minerals and hemolytic activity.
Ancient stone stela, which was on conservation treatment during the study period, was
investigated and a high degree o...f biological colonization as well as deterioration of stone
substrata was detected, corresponding to obtained index of deterioration value.
Additionally, production of acid and alkaline metabolites, pigment production, carbonate
solubilization and biogenesis of secondary mycogenic minerals was determined by
autochtonous isolates from stone stela. Finally, antifungal potential of selected essential
oils, metyl salicylate and benzalconium chloride was evaluated on airborne Aspergilli and
Penicillia. The strongest antifungal activity was demonstrated for benzalconium chloride
and Cinnamonum zeylanicum essential oil. Results of the molecular docking of the main
essential oils componnents were in excellent correlation with the documented antifungal
activity of the examined biocides.
Факултет:
Универзитет у Београду, Биолошки факултетДатум одбране:
15-11-2019Пројекти:
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)