Terapijski efekti eritropoetina u direktnom prekrivanju inflamirane zubne pulpe
Therapeutical effects of erythropoietin in direct pulp capping of inflamed dental pulp
Докторанд
Papić, MilošМентор
Popović, MilicaЧланови комисије
Ljujić, BiljanaTanasković, Irena
Radović, Ivana
Метаподаци
Приказ свих података о дисертацијиСажетак
Uvod Direktno prekrivanje predstavlja postavljanje određenih materijala na mesto
eksponiranja pulpe kako bi se održao njen vitalitet. Stopa uspeha terapije značajno
se umanjuje ako se sprovodi na prethodno inflamiranoj zubnoj pulpi. Eritropoetin
(EPO) je glavni stimulator eritropoeze, ali postojeća saznanja ukazuju da može
ostvariti različite povoljne efekte u reparaciji oštećenih tkiva.
Cilj Ispitivanje ekspresije eritropoetina i eritropoetinskog receptora u
eksponiranoj i direktno prekrivenoj pulpi i ispitivanje efekata eritropoetina na
inflamaciju i reparatornu dentinogenezu u terapiji direktnim prekrivanjem
inflamirane zubne pulpe.
Materijal i metode Indukovana je inflamacija eksponiranjem pulpe prvih
maksilarnih molara pacova na 48 sati, a zatim direktno prekrivanje primenom rhEPO,
Ca(OH)2, MTA ili MTA+rhEPO. Kao kontrole korišćene su eksponirana
neprekrivena (EC) i zdrava zubna pulpa (HC). Sprovedene su imunohistohemijska,
histološka, PCR i spektrofotometrijska analiza. Dobijeni ...podaci su obrađeni
statistički.
Rezultati EC grupa imala je najvišu ekspresiju EPO-R, dok je prekrivanje primenom
Ca(OH)2 ili MTA dovelo do povećanja ekspresije EPO. Direktno prekrivanje umanjilo
je ekspresiju proinflamatornih citokina i povećalo ekspresiju imunoregulatornih
citokina u odnosu na EC grupu. Primena MTA+rhEPO dovela je do povećanja
ekspresije markera odontoblaste aktivnosti u odnosu na EC grupu. Primena
preparata koji sadrže rhEPO umanjila je vrednosti prooksidativnih parametara u
odnosu na EC grupu.
Zaključak ERO i ERO-R eksprimirani su u eksponiranoj i direktno prekrivenoj
zubnoj pulpi. EPO može biti pogodan za primenu u terapiji direktnim prekrivanjem
pulpe jer umanjuje stepen inflamacije i stimuliše reparatornu dentinogenezu,
ostvarujući efekat sličan uobičajeno korišćenim materijalima.
Introduction Direct pulp capping presents placing of certain materials on exposure site in
order to preserve its vitality. The success rate of this therapy is significantly reduced if it is
performed on previously inflamed dental pulp. Erythropoietin (EPO) is the main stimulator of
erythropoiesis, but the existing literature indicates that it could have various beneficial effects
on repair of damaged tissues.
Aim To investigate the expression of erythropoietin and erythropoietin receptor in pulp
exposure and direct pulp capping and to investigate the effects of erythropoietin on
inflammation and reparative dentinogenesis in direct capping of inflamed dental pulp.
Material and methods Pulp inflammation was induced by exposing the pulp of rats’
maxillary first molars for 48 h, followed by capping using rhEPO, Ca(OH)2, MTA or
MTA+rhEPO. Noncapped exposed pulp (EC) and healthy pulp (HC) were used as controls.
Immunohistochemical, histological, PCR and spectrophotometric analysis were perfo...rmed.
Acquired data was analyzed statistically.
Results EC group had the highest expression of EPO-R, while capping with Ca(OH)2 or
MTA led to increased EPO expression. Direct pulp capping decreased the expression of proinflammatory and increased the expression of immunoregulatory cytokines compared to EC
group. Capping with MTA+rhEPO led to increased expression of odontoblast activity
markers compared to EC group. Capping with the rhEPO-containing materials reduced the
values of pro-oxidative parameters compared to EC group.
Conclusion EPO and EPO-R are expressed in exposed and capped dental pulp. EPO can be
suitable for direct pulp capping because it reduces the degree of inflammation and stimulates
the reparative dentinogenesis, having similar effects to commonly used materials.