Mikro RNK-21 kao indikator invazivnosti karcinoma dojke kod pacijenata sa različitim statusom estrogenskog i progesteronskog receptora
MicroRNA-21 as an indicator of breast carcinoma invasiveness in patients with different estrogen and progesterone receptor status.
Докторанд
Petrović, Nina M.Ментор
Mandušić, VesnaЧланови комисије
Brajušković, GoranSavić-Pavićević, Dušanka
Stanojević, Boban
Метаподаци
Приказ свих података о дисертацијиСажетак
Karcinomi dojke su izuzetno heterogeni tumori koji se na osnovu sposbonosti invazivnosti
dele na neinvazivne, invazivne asocirane sa neinvazivnim i invazivne. Najznačajniji
standardni dijagnostički i prognostički parametri karcinoma dojke su veličina tumora,
starost pacijentkinje, status regionalnih limfnih čvorova, gradus tumora, status
estrogenskog receptora (ER), progesteronskog receptora (PR), receptora za humani
epidermalni faktor rasta 2 (Her-2) i status proliferativnog indeksa (Ki-67). Male
nekodirajuće mikro RNK igraju važnu ulogu u regulaciji velikog broja bioloških procesa, a
poremećaji u njihovoj ekspresiji detektovani su u procesima nastanka i progresije malignih
tumora. Cilj ove disertacije je da se ispita nivo i značaj ekspresije onkogene mikro RNK-21
(miR-21) u tumorskom tkivu dojke. Imunohistohemijski je analiziran nivo ekspresije
proteina ER, PR, Her-2 i Ki-67 u neizmenjenom i tumorskom tkivu dojke dok je nivo
ekspresije miR-21 određivan lančanom rekacijom polimeraze k...ojoj prethodi reverzna
transkripcija korišćenjem prajmera sa petljom. Razlike u nivoima ekspresije miR-21 u
uzorcima neinvazivnih i invazivnih karcinoma dojke sa in situ koponentom ukazuju na
moguću biološku ulogu miR-21 u procesima odgovornim za invazivnost karcinoma dojke,
odnosno da nvo ekspresije molekulа mikro RNK-21 predstаvljа potencijаni biološki mаrker
invаzivnosti kаrcinomа dojke. Rezultаt dа u аnаlizirаnoj grupi čistih invаzivnih kаrcinomа
dojke postoji visok stepen pozitivne korelаcije između nivoа ekspresije molekulа mikro
RNK-21 sа nivoom ekspresije proteinа ER i PR, ukаzuje nа to dа nivo ekspresije molekulа
mikro RNK-21 može predstаvljаti novi nestаndаrdni dijagnostički i prognostički pаrаmetаr
kаrcinomа dojke.
Breast cancer is a complex and heterogeneous group of tumors. According to their ability to
invade, breast cancers are divided in three major types: non-invasive, invasive carcinoma of
the breast associated with non-invasive and invasive breast carcinomas. The most
significant standard diagnostic and prognostic parameters of the breast carcinomas are size
of a tumor, age at diagnosis, regional lymph-node status, tumor grade, estrogen receptor
(ER), progesterone receptor (PR), human epidermal growth factor 2 receptor status and
proliferative index Ki-67 status. Small non-coding microRNAs play very important part in
the regulation of great number of biological processes. Deregulation in their expression are
detected in the process of malignant tumor formation and progression. Main goal of this
dissertation was to investigate expression levels of microRNA-21 (miR-21) in the tumorous
breast tissue and its potential role in breast tumor formation and invassion. Expression
levels of ER, PR, ...Her-2 and Ki-67 proteins were detected by immunohistochemistry in
non-transformed and tumorous tissue of the breast. MicroRNA-21 expression levels were
determined by the reaction of Reverse Transcription and Stem-Loop Polymerase Chain
Reaction. The differences in expression levels of miR-21 among non-invasive, invasive
breast carcinoma associated with non-invasive and invasive breast carcinomas emphasizes
potential biological role of miR-21 in the proces of breast cancer invasiveness and indicates
that expression levels of microRNA-21 might represent potential biomarker of invasiveness
in the breast carcinomas. We have found that there was highly positive correlation in miR-
21 expression levels with ER and PR protein expression levels, which indicates that miR-
21 expression levels might be used as novel non-standard parameter for breast cancer
diagnosis and prognosis.
Факултет:
Универзитет у Београду, Биолошки факултетДатум одбране:
29-09-2014Пројекти:
- Молекуларне детерминанте за дизајн тумор маркера (RS-MESTD-Basic Research (BR or ON)-173049)