Genotyping, antibiotic resistance and biofilm formation of Stenotrophomonas maltophilia clinical isolates from Serbia
Генотипизација, резистенција на антибиотике и формирање биофилма клиничких изолата Stenotrophomonas maltophilia из Србије
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Stenotrophomonas maltophilia is an environmental bacterium and an
opportunistic pathogen usually associated with healthcare-associated
infections, which has recently been recognized as a global multidrug resistant
organism. The aim of this study was genotyping and physiological
characterization of S. maltophilia collected during the routine health care at The
Institute for Mother and Child Health Care of Serbia “Dr Vukan Čupić“. It is the
large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national
reference cystic fibrosis (CF) center for pediatric and adult patients.
We characterized 88 S. maltophilia strains, 42 strains of cystic fibrosis (CF)
and 46 strains of non-cystic fibrosis (non-CF) origin isolated from 2013 to 2015
in order to investigate their genetic relatedness and phenotypic traits.
Genotyping was performed using sequencing of 16S rRNA gene, Pulse Field
Gel Electrophoresis (PFGE) and Multi locus sequencing typing (MLST) analysis.
Sensitivity to five ...relevant antimicrobial agents was determined, namely
trimethoprim/sulfamethoxazole (TMP/SMX), chloramphenicol, ciprofloxacin,
levofloxacin and tetracycline. Surface characteristics, motility, biofilm formation
and adhesion to mucin were tested in all strains. In addition, influence of
different factors (temperature, pH, agitation and CO2) on biofilm formation,
kinetics of selected biofilm producers and effect of TMP/SMX on formed
biofilm were analyzed. Statistical approach was used to determine correlations
between obtained results.
All analyzed clinical isolates belong to S. maltophilia species with identity
ranging from 95% to 99% with S. maltophilia strains from the NCBI database
what was confirmed by sequencing of PCR products of amplified 16S rRNA
gene...