Farmakodinamska ispitivanja magnezijum-sulfata i dizocilpina u modelima somatskog i visceralnog bola kod pacova
Pharmacodynamic evaluation of magnesium sulfate and dizocilpine in models of somatic and visceral pain in rats
Author
Srebro , Dragana P.Mentor
Vučković, SonjaCommittee members
Prostran, MilicaStojanović, Radan
Janković, Slobodan
Metadata
Show full item recordAbstract
Magnezijum je mineral koji u organizmu ima brojne uloge. On je kofaktor u više
od 300 enzimskih reakcija, održava membranski potencijal, ima modulatorno dejstvo na
jonske kanale, neurotransmisiju, i drugo...
Magnesium is a mineral who has many functions in the human body: he
is cofactor for 300 enzymatic reaction, modulates ion channels, neurotransmission, etc.
Magnesium is an endogenous voltage-dependent N-methyl-D-aspartate (NMDA)
receptor channel blocker. Dizocilpine (MK-801) is a noncompetitive NMDA receptor
antagonist. Dizocilpine is commonly used as a neuropharmacological tool. The
activation of NMDA receptor has a significant role in the development and maintenance
of an inflammatory pain. Activation of these receptors may increase the synthesis of
nitric oxide (NO). Also, transient receptor potential (TRP) channels and acid sensitive
ion channels (ASIC) are important in pain. It is well known that magnesium and
dizocilpine can reduce neuropathic pain and enhance analgesic effect of opioids or
anesthetics.
Aim: The aim of this thesis was to examine whether magnesium sulfate and dizocilpine
has analgesic and antiedematous effect in somatic and visceral inflammatory pain in rats
and t...o explore the possible role of NO in the mechanisms of their actions.
Methods: Male Wistar rats were used. Carragennan-induced hyperalgesia and edema in
rats was used as a model of somatic inflammatory pain. Hyperalgesia was examined by
von Fray analgesiometric test. Antiedematous effect was examined by immersing paw
in the plethysmometer. The acetic acid-induced writhing test in rats was used as a model
of visceral pain. Mechanism of action was evaluated with inhibitors of NOS.
Results: In the somatic inflammatory model of pain systemically administered
magnesium sulfate reduces hyperalgesia and edema in a dose-independent manner. It is
more efficient in lower (5 mg/kg, sc), than in higher doses (15 and 30 mg/kg, sc). The
analgesic effect is not present after local peripheral application, as opposed to
antiedematous effect that is present after both routes of administration. Magnesium
sulfate systematically applied has preventive (given as a pretreatment) and therapeutic
(given as treatment) effect on pain and swelling in inflammation. Dizocilpine has dosedependent
antihyperalgesic effect after systemical and local peripheral application in the
model of somatic inflammation...
Faculty:
Универзитет у Београду, Медицински факултетDate:
04-04-2016Projects:
- Basic and Clinical Pharmacological research of mechanisms of action and drug interactions in nervous and cardiovascular system (RS-MESTD-Basic Research (BR or ON)-175023)