Vrste roda Trichoderma, uzročnici zelene plesni šampinjona [Agaricus bisporus (Lange) Imbach] i njihova osetljivost na fungicide i biofungicide
Trichoderma species causing green mould disease of button mushroom [Agaricus bisporus (Lange) Imbach] and their sensitivity to fungicides and biofungicides
Докторанд
Kosanović, Dejana D.Ментор
Potočnik, IvanaЧланови комисије
Vukojević, JelenaStajić, Mirjana
Duduk, Bojan
Метаподаци
Приказ свих података о дисертацијиСажетак
Pojedine vrste roda Trichoderma su uzročnici zelene plesni šampinjona (Agaricus
bisporus) i nanose velike materijalne štete u gajilištima. Od 2006. do 2010. godine
prikupljeno je 20 izolata roda Trichoderma iz 13 gajilišta u Srbiji i jednog iz Bosne i
Hercegovine. Dvanaest izolata je klasifikovano na osnovu standardnih mikoloških
metoda i analizom ITS1/ITS4 sekvence u pet vrsta: Trichoderma atroviride, Trichoderma
koningii, Trichoderma virens, Trichoderma aggressivum f. europaeum i Trichoderma
harzianum. Osam izolata nije identifikovano do nivoa vrste ali je pokazano da su
filogenetski bliski sa vrstom T. harzianum. Najvišu virulentnost na ubranom šeširu A.
bisporus je pokazala vrsta T. harzianum, a najnižu T. virens i T. aggressivum f.
europaeum.
Većina izolata je najbolje rasla na podlozi koja je imala pH = 5, potom na pH = 6,
par izolata na pH = 7, dok je najslabiji rast zabeležen na pH = 8 – 9. Generalno, svetlost
je imala inhibitorni efekat na rast izolata. Fungicidi su u širokoj ...upotrebi u kontroli
bolesti šampinjona, iako je kontrola zelene plesni otežana. Stoga, jedan od ciljeva
istraživanja je bio da se ispita in vitro toksičnost nekoliko dostupnih komercijalnih
fungicida na prikupljene izolate roda Trichoderma. Testirani izolati su bili najosetljivi na
prohloraz-Mn, hlorotalonil i karbendazim (EC50 1 mg/L), osetljivi na iprodion (EC50 =
0,84 – 6,72 mg/L), umereno rezistentni na tiofanat-metil (EC50 = 3,75 – 24,13 mg/L) i
rezistentni na trifloksistrobin (EC50 = 10,25 – 178,23 mg/L). Proučavajući toksičnost
fungicida na A. bisporus, najbolju selektivnu toksičnost su pokazali prohloraz-Mn (0,05) i
karbendazim (0,02), iprodion i hlorotalonil umerenu (0,16), tiofanat-metil najnižu (1,24),
dok toksičnost trifloksistrobina za A. bisporus nije testirana zbog nezadovoljavajuće
toksičnosti za Trichoderma spp. izolate.
Najbolju selektivnu toksičnost za patogenu gljivu i gljivu domaćina su pokazali
prohloraz-Mn i karbendazim sa vrednostima nižim od 0,1. Pošto se karbendazim povlači
sa tržišta, prohloraz-Mn je fungicid koji se može preporučiti za primenu u gajilištima za
suzbijanje zelene plesni.
Antifungalna aktivnost dva biofungicida, na bazi Bacillus subtilis i ulja čajnog
drveta, je testirana in vitro na sve Trichoderma spp. izolate. B. subtilis je bio visoko
toksičan za sve testirane izolate (EC50 < 1,3 mg/L), dok ulje čajnog drveta nije pokazalo
značajnu antifungalnu aktivnost (EC50 = 11,9 – 370,8 mg/L).
Efikasnost biofungicida protiv vrste T. harzianum je ocenjena u oglednom
gajilištu. Biofungicidi su primenjeni zasebno ili u kombinaciji sa fungicidom, prohloraz-
Mn, u proporciji 20:80%. Prohloraz-Mn se pokazao efikasnijim od oba biofungicida kao i
od kombinovane primene biofungicida i fungicida. Biofungicid na bazi B. subtilis je bio
efikasniji od ulja čajnog drveta u pogledu sprečavanja pojave simptoma bolesti. Takođe,
B. subtilis je pokazao manji antagonistički efekat u efikasnosti protiv patogena od ulja
čajnog drveta kada se primenjuju u kombinaciji sa prohloraz-Mn fungicidom.
Trichoderma species, the causal agents of green mould disease, induce great
losses in button mushroom (Agaricus bisporus) farms. Twenty Trichoderma isolates were
collected on 13 Serbian A. bisporus farms and one in Bosnia and Herzegovina during
2006 – 2010. Twelve isolates were classified into five species by standard mycological
studies and ITS1/ITS4 sequence analyses, namely Trichoderma atroviride, Trichoderma
koningii, Trichoderma virens, Trichoderma aggressivum f. europaeum and Trichoderma
harzianum. Eight isolates were not identified to the species level but were shown to be
related to T. harzianum. The isolates of T. harzianum exhibited the highest virulence to
the harvested A. bisporus pilei and T. virens and T. aggressivum f. europaeum the lowest.
Fungicides are widely used to control mushroom diseases although green mould
control is encumbered with difficulties. The aims of this study were therefore to research
in vitro toxicity of several commercial fungicides to Trichoderma ...isolates originating
from Serbian and Bosnia-Herzegovina farms, and to evaluate the effects of pH and light
on their growth. The majority of isolates demonstrated optimal growth at pH 5.0, and the
rest at pH 6.0. A few isolates also grew well at pH 7. The weakest mycelial growth was
noted at pH 8.0 – 9.0. Generally, light had an inhibitory effect on the growth of tested
isolates. The isolates showed the highest susceptibility to prochloraz-Mn, chlorothalonil
and carbendazim (ED50 less than 1 mg/L), and were less sensitive to iprodione (ED50
ranged 0.84 – 6.72 mg/L), weakly resistant to thiophanate-methyl (ED50 = 3.75 – 24.13
mg/L), and resistant to trifloxystrobin (ED50 = 10.25 – 178.23 mg/L). Considering the
toxicity of fungicides to A. bisporus, prochloraz-Mn and carbendazim showed the best
selective toxicity (0.05 and 0.02 respectively), iprodione and chlorothalonil moderate
(0.16), and thiophanate-methyl the lowest (1.24), while trifloxystrobin toxicity to A.
bisporus was not tested because of its inefficiency against Trichoderma isolates.
The best selective toxicity to both pathogen and the host showed prochloraz-Mn
and carbendazim with values less than 0.1. Carbendazim was withdrawn from the market
and therefore prochloraz-Mn could be recommended for mushroom cultivation in Serbia.
Antifungal activity of two biofungicides based on Bacillus subtilis and tea tree oil
were tested in vitro to all Trichoderma isolates. B. subtilis was highly toxic to all tested
Trichoderma isolates, their ED50 values were below 1.3 mg/L. Tea tree oil did not exhibit
a significant antifungal activity (ED50 = 11.9 – 370.8 mg/L).
The efficacy of biofungicides was evaluated against T. harzianum in a mushroom
growing room, and they were applied alone or in combination with the fungicide at a
respective proportion of 20:80%. Prochloraz-Mn showed higher efficacy than both tested
biofungicides or their respective mixtures. The biofungicide based on B. subtilis
demonstrated greater efficacy in preventing disease symptoms than tea tree oil. B. subtilis
combined with the fungicide revealed less antagonism in efficacy against pathogen than
tea tree oil.