Masnokiselinski profili fosfolipida i parametri oksidativnog stresa u krvi i jetri pacova Wistar soja tretiranih ribljim uljem
Fatty acids phospholipids profiles and parameters of oxidative stress in blood and liver in Wistar rats treated with fish oil
Doktorand
Popović, Tamara B.Mentor
Mandić, LjubaČlanovi komisije
Glibetić, MarijaSpasić, Mihajlo
Vrvić, Miroslav
Borozan, Sunčica
Metapodaci
Prikaz svih podataka o disertacijiSažetak
Polinezasićene masne kiseline, posebno eikozapentaenska (EPA) i dokozaheksaenska (DHA)
masna kiselina koje ulaze u sastav ribljeg ulja i pripadaju n-3 familiji masnih kiselina, imaju
efekte na čitav niz fizioloških parametara. Značaj balansa odnosa n-6/n-3 masnih kiselina u
fosfolipidima kao i balans izmeñu produkcije reaktivnih kiseoničnih vrsta i reaktivnih azotnih
vrsta s jedne i antoksidativne zaštite s druge strane je od važnosti za normalno funkcionisanje
organizma.
Suplementirali smo pacove Wistar soja dve starosne dobi (3 i 22 meseca) u trajanju od 6 nedelja
sa ribljim uljem (EPA+DHA i vitamin E). Ispitivani su biohemijski parametri plazme,
masnokiselinski profili fosfolipida plazme i jetre kao i parametri oksidativnog stresa u krvi i jetri
pomenutih životinja.
Dobijeni rezultati su pokazali više vrednosti holesterola, HDL- holesterola i triglicerida u plazmi
mladih pacova u odnosu na stare. Tretman ribljim uljem doveo je do povišenja HDL-holesterola,
koncentracije mokraćne kis...eline i sniženja LDL-holesterola kod obe grupe tretiranih životinja
dok je kod mladih tretman izazvao sniženje koncentracije triglicerida a kod starih sniženje
holesterola.
Starenjem, povećava se sadržaj linolne, arahidonske kiseline kao i ukupnih n-6 MK. Tretman
ribljim uljem, doveo je do povećanja sadržaja EPA, DPA, sniženja koncentracije arahidonske
kiseline u fosfolipidima plazme u obe starosne grupe životinja. Sadržaj oleinske kiseline je kod
mladih pacova tretmanom povižen, dok je kod starih pacova došlo do povećanja dihomo-γ-
linolenske kiseline. Starenjem, snižava se i sadržaj ukupnih n-3 MK i odnos n-6/n-3 u fosfolipidima jetre. Tretman u životinja obe starosne dobi doveo je do povećanja sadržaja EPA,
DPA, ukupnih n-3, smanjenja sadržaja arahidonske kiseline u fosfolipidima jetre. Kod mladih
tretman je povećao sadržaj MUFA, a kod starih SFA i PUFA.
Starenjem, povećava se lipidna peroksidacija u hepatocitima, kao i aktivnost SOD i koncentracija
nitrita, dok je aktivnost PON1 u plazmi veća kod mladih pacova što je u korelaciji sa višim
vrednostima HDL-holesterola u odnosu na stare pacove. Tretman ribljim uljem kod pacova obe
starosne dobi doveo je do sniženja lipidne peroksidacije, koncentracije plazma nitrita i
zastupljenosti LDH5 izoenzimskog oblika a do povišenja aktivnosti SOD, CAT i PON1 u krvi
tretiranih životinja u odnosu na kontrolnu grupu...
Polyunsaturated fatty acids, especially eicosapentanoic acid (EPA) and dosahexanoic acid
(DHA) which are the content of fish oil and belong to the n-3 fatty acid family. n-3 are known to
have effect on physiological parameters. The balance of the n-6/n-3 ratio in phospholipids, as
well as the balance between reactive oxygen species and reactive nitric species on the one side
and on the anti oxidative defense on the other is important for the normal function of organism.
Wistar rats (3 and 22 months) were supplemented with fish oil (EPA+DHA and vitamin E) over
the period of 6 weeks. Biochemical parameters in plasma, fatty acid phospholipids profiles and
the parameters of oxidative stress in blood and liver were examined.
In young rats plasma concentrations of cholesterol, HDL-cholesterol and triglycerides in plasma
were increased compared to aged rats. Fish oil treatment increased HDL-cholesterol, uric acid
and LDL-cholesterol in both groups of animals (3 and 22 months). Treatment decre...ased
triglycerides concentrations in young rats and decreased plasma cholesterol in aged rats.
Senescence increased LA, AA and n-6 FA. Fish oil treatment increased EPA, DPA, decreased
AA in plasma phospholipids in both group of animals. In young rats treatment increased oleic
acid while in aged it increased DGLA. Senescence decreased n-3 FA and the n-6/n-3 ratio in
liver phospholipids. Treatment increased EPA, DPA, n-3 and decreased AA in liver
phospholipids in both group of animals. In young rats, treatment increased MUFA while in aged
rats it increased SFA and PUFA.
Senescence also increased lipid peroxidation in liver cells, as well as SOD activity and nitrite
concentration while the activity of PON1 in plasma is increased in young rats compared to aged
Wistar rats. Fish oil treatment in both groups of rats (3 and 22 months) decreased lipid
peroxidation, concentration of plasma nitrite and LDH5 and increased SOD, CAT, PON1 activity in blood. Treatment decreased lipid peroxidation, nitrite concentration in liver of both
groups of rats. In young rats treatment also decreased CAT activity and increased PON1. In aged
rats treatment increased CAT, SOD activity, concentration of SH groups while PON activity
decreased compared to control...