Povezanost ekspresije galektina-3 i inflamacijskih citokina u preterminskom porođaju sa horioamnionitisom
Assotiation between the expression of galectin-3 and inflammatory cytokines in preterm delivery with chorioamnionitis
Докторанд
Jović, NikolaМентор
Varjačić, MirjanaЧланови комисије
Miković, ŽeljkoMilovanović, Jelena
Babić, Goran M.
Метаподаци
Приказ свих података о дисертацијиСажетак
Patogenetski mehanizmi nastanka preterminskog porođaja (PP) su nedovoljno
ispitani. Najčešći razlog PP je inflamacijska reakcija, predominantno po tipu
horioamnionitisa, u kojoj učestvuju inflamacijski citokini IL-1β i IL-6. Iako je u
raznim akutnim i hroničnim inflamacijskim bolestima pokazano da GAL-3
stimulaciše produkciju IL-1β i IL-6, nije ispitivana udruženost ekspresije GAL-3 i
ovih citokina u slučajevima PP.
Analizirali smo ekspresiju IL-1β, IL-6 i GAL-3 u tkivu posteljice i plodovih
ovojaka, kao i koncentraciju ovih molekula u serumu dobijenom iz periferne krvi majke
i krvi pupčanika. Ispitivali smo korelaciju između ekspresije IL-1β, IL-6, GAL-3 u
tkivu posteljice i plodovih ovojaka, kao i koncentracije u serumu međusobno, ali i sa
biohemijskim markerima inflamacije (Le, CRP, fibrinogen) iz krvi majke i
pupčanika.
Naša studija je pokazala povećanu ekspresiju IL-1β i GAL-3 u tkivu posteljice,
ali i u serumu majke i serumu pupčanika u grupi sa preterminskim porođaje...m i
dokazanim horioamnionitisom, u odnosu na kontrolnu grupu. Takođe utvrđena je
pozitivna korelacija između IL-1β i GAL-3 u navedenim tkivima međusobno, kao i sa
biohemijskim markerima inflamacije (leukociti, CRP, fibrinogen) iz krvi majke i
pupčanika. Naša studija nije našla korelaciju između ekspresije IL-6 u tkivu
posteljice i plodovih ovojaka i koncentracije ovog citokina iz seruma majke i
pupčanika sa ekspresijom i koncentracijom IL-1β i GAL-3 u navedenim tkivima.
Naša studija je prva koja se pokazala ekspresiju ispitivanih citokina i njihovu
međusobnu korelaciju u različitim kompartmanima posteljice (plodovi ovojci,
horionske resice i decidua), kao i njihovu korelaciju sa biohemijskim markerima
inflamacije.
Pathogenetic mechanisms of preterm birth (PB) have been insufficiently investigated.
The most common cause of PB is an inflammatory reaction, predominantly chorioamnionitis,
where the inflammatory cytokines IL-1β and IL-6 participated. Although it has been shown in
various acute and chronic inflammatory diseases that GAL-3 stimulates the production of IL1β and IL-6, the association between the expression of GAL-3 and these cytokines in PB has
not been investigated yet.
We analyzed the expression of IL-1β, IL-6 and GAL-3 in the tissue of the placenta and
fetal membranes, as well as the concentration of these molecules in the serum obtained from
maternal peripheral blood and umbilical cord blood. We examined the correlation between the
expression of IL-1β, IL-6, GAL-3 in the tissue of the placenta and fetal membranes, as well as
their serum concentrations, but also with biochemical markers of inflammation (WBC, CRP,
fibrinogen) from maternal and umbilical cord blood.
Our study sh...owed increased expression of IL-1β and GAL-3 in placenta tissue, but also
in maternal and umbilical cord serum in the group with preterm delivery and proven
chorioamnionitis, compared to the control group. A positive correlation was also established
between IL-1β and GAL-3 in the mentioned tissues, as well as with biochemical markers of
inflammation (WBC, CRP, fibrinogen) from maternal and umbilical cord blood. Our study did
not find a correlation between the expression of IL-6 in the tissue of the placenta and fetal
membranes and the serum concentration of this cytokine from mother and the umbilical cord
samples with the expression and concentration of IL-1β and GAL-3 in the mentioned tissues.
Our study is the first to demonstrate the expression of the investigated cytokines and
their mutual correlation in different compartments of the placenta (fetal membranes, chorionic
villi and decidua), as well as their correlation with biochemical markers of inflammation.