Ispitivanje efikasnosti akrilata modifikovanog antigljivičnim supstancama u sprečavanju kolonizacije proteza gljivama roda Candida
Testing the efficiency of acrylate modified by antifungal substances in preventing Candida spp. colonization on denture
Докторанд
Išljamović, MilicaМентор
Todić, JelenaЧланови комисије
Živković, RadeOtašević, Suzana
Milosavljević, Marko
Метаподаци
Приказ свих података о дисертацијиСажетак
Uvod: Nošenje zubne proteze je predisponirajući faktor za razmnožavanje
gljiva roda Candida na oralnoj mukozi i razvoj proteznog stomatitisa. Problem u
terapiji proteznog stomatitisa je rezistencija gljiva i nemogućnost postizanja
efikasnih koncentracija antimikotika na protezi.
Cilj je izvršiti modifikaciju hladnopolimerizujućeg akrilata (PMMA) za
podlaganje zubnih proteza dodavanjem antigljivičnih supstanci (AS): undecileinske
kiseline (UK), farnezola (F) ili njihove kombinacije (UK+F); ispitati fizičkohemijske osobine modifikovanih akrilata (MA), citotoksičnost i antifungalni
efekat na soj Candida (C.) albicans ATCC 90028.
Materijal i metode: fizičko-hemijska karakterizacija površine MA je
urađena pomoću totalne refleksije u infracrvenoj spektroskopiji sa Furijeovom
transformacijom (FTIR) i merenjem vodenog kontaktnog ugla površine MA.
Citotoksičnost MA je ispitana pomoću MTS-[3-(4,5-dimetiltiazol-2-il)-5-(3-
karboksimetoksifenil)-2-(4-sulfofenil)-2H-tetrazolijum] testa,... a antigljivična
aktivnost testom disk difuzije. Efekat MA na sesilne i planktonske ćelije gljiva
vrste C.albicans je ispitan pomoću XTT [2,3-bis (2-metoksi–4–nitro–5–sulfofenil)-
2H-tetrazolium-5-karboksanilid)] testa.
Rezultati: Antigljivične supstance su prisutne na površini MA i značajno
povećavaju hidrofilnost u odnosu na kontrolu. AS nisu ispoljile citotoksičnost u
koncentraciji koja utiče na redukciju metabolički aktivnih planktonskih i
sesilnih ćelija vrste C. albicans na površini MA. Kombinacija UK+F omogućava
primenu nižih koncentracija AS u PMMA. Ove supstance prisutne u agaru
sprečavaju prelazak vrste C. albicans u filamentoznu formu rasta.
Zaključak: modifikovani hladnopolimerizujući akrilati za podlaganje zubnih
proteza bi mogli da unaprede tretman proteznog stomatitisa izazvanog gljivama roda
Candida.
Introduction: Wearing denture is accompained with colonisation of Candida spp. and the development of Candida-associated denture stomatitis. The problem in therapy is fungal resistance and the inability to achieve effective antifungal concentrations on denture.
Aim is to modify cold-curing acrylic resin (PMMA) for denture lining with antifungal substances (AS): undecylenic acid (UA), farnesol (F) or their combination (UA + F), to study
the physico-chemical properties of the modified acrylates (MA), cytotoxicity and its
antifungal effect on Candida (C.) albicans ATCC 90028.
Material and methods: physico-chemical characterization of the MA surface was
characterized by Fourier transform infrared spectroscopy (FTIR), by measuring the water
contact angle; toxicity of the MA was tested by using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-
carboxymethoxypenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) test, while antifungal
activity was elavuated by disc diffusion test. The effect of the MA on... sessile and planktonic C. albicans cells was examined using XTT (2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2Htetrazolium-5-carboxanilide) reduction assay.
Results: Аntifungal substances were observed on the surface of MA and significantly reduced
the contact angle compared to control (PMMA). AS are not cytotoxic in a concentration that
will affect the reduction of metabolically active planktonic and sessile C. albicans cells on the
МА surface. UA+F combination allows the application of lower concentrations of AS in
PMMA. AS inhibit transition of C. albicans cells from yeast to filamentous form.
Conclusion: Modified cold-curing acrylic resin for dentures lining could improve the
treatment of denture stomatitis.