Patomorfološka, mikološka i molekularna ispitivanja organa ćurića različitog imunološkog statusa nakon veštačke infekcije sporama Aspergillus fumigatus
Pathological, mycological and molecular research into the organs of turkey poults of various immunological status following artificial infection with Aspergillus fumigatus spores
Докторанд
Jezdimirović, NemanjaМентор
Jovanović, MilijanЧланови комисије
Krnjaić, DejanKureljušić, Branislav
Jovanović, Milijan
Метаподаци
Приказ свих података о дисертацијиСажетак
Aspergiloza ćuraka je jedno od najvažnijih respiratornih oboljenja, naročito kod
najmlađe kategorije, koje može da prouzrokuje značajne ekonomske gubitke zbog
mortaliteta i niskih proizvodnih performansi. U prirodnim uslovima infekcija nastaje
aerogeno, tj. udisanjem velikog broja germinativnih oblika spora Aspergillus fumigatus
u kratkom vremenskom periodu ili dugotrajnim izlaganjem respiratornog sistema niskoj
koncentraciji u kontaminiranom vazduhu. Predisponirajući faktori za nastanak
aspergiloze kod ćuraka, pored virusnih i bakterijskih infekciija jesu: slab imuni status
nastao zbog duže primene antibakterijskih lekova, kontinuirano izlaganje stresogenim
faktorima (učestala vakcinacija, loš kvalitet hrane), izlaganje lošim ambijentalnim i
sanitarnim uslovima uzgoja.
Iako postoji mnogo radova o aspergilozi ćurića, malo se zna o patogenezi bolesti
i patomorfološkim promenama u različitim tkivima kod imunosuprimiranih ptica.
Imajući to u vidu zadatak ovog rada bio je da u eksperimenta...lnim uslovima ispita
zdravstveno stanje, nastanak patomorfoloških promena i diseminaciju Aspergillus
fumigatus u različitim organima kod imunosuprimiranih i imunokompetentnih ćurića.
Ogled je izveden na ukupno 90 ćurića oba pola, provenijencije „Converter“,
podeljenih u četiri grupe: ogledna grupa 1 (O-1) i ogledna grupa 2 (O-2) sa po 30
jedinki i kontrolne grupe 1 (K-1) i 2 (K-2) sa po 15 jedinki u svakoj. Ispitivanja su
obavljena 1, 3, 7, 14. i 21. dana nakon intratrahealne inokulacije 5,056×107 spora A.
fumigatus 14 dana starim ćurićima grupe O-1, kao i grupe O-2, koja je prethodno
tretirana deksametazonom u dozi od 4mg/kg telesne mase intramuskularno u trajanju od
šest uzastopnih dana...
Aspergillosis is one of the most important respiratory diseases of turkeys,
especially in the youngest age category. It may lead to significant economic losses due
to mortality and poor production performance. In natural conditions the infection is
aerogenous, that is, it occurs following the inhalation of large numbers of Aspergillus
fumigatus germinating spores in a short period of time, or by long-lasting exposure of
the respiratory system to their low concentrations in air. Apart from virus and bacterial
infections, the predisposing factors for the emergence of aspergillosis in turkeys are:
low immune status due to long-lasting use of antibacterial drugs, continual exposure to
stressogenic factors (frequent vaccination, poor feed quality), and exposure to poor
ambient and sanitary conditions of rearing.
In spite of plenty works about turkey aspergillosis, little is known about the
pathogenesis of the disease and the pathomorphological changes in various tissues in
immunosuppressed ...birds. Having considered these, this work was aimed at the research
into the health status, development of pathological changes and dissemination of
Aspergillus fumigatus in various organs of immunosuppressed and immunocompetent
turkey poults in experimental conditions.
The experiment was performed on 90 turkey poults of both sexes, of the
Converter hybrid, divided into four groups: treated groups 1 (O-1) and 2 (O-2) with 30
specimens in each, and the two control groups – group 1 (K-1) and group 2 (K-2) - each
consisting of 15 birds. The analyses were done on days 1, 3, 7, 14 and 21 after
intratracheal inoculation of 5.056×107 A. fumigatus spores to 14-day-old poults of group
O-1 and those of O-2, which were intramuscularly administered dexamethasone in the
dose of 4 mg/kg body weight, on six consecutive days...