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Diversity and characterization of tomato viruses in Serbia

dc.contributor.advisorKrstić, Branka
dc.contributor.otherStanković, Ivana
dc.contributor.otherDelibašić, Goran
dc.contributor.otherMoravčević, Đorđe
dc.contributor.otherRistić, Danijela
dc.creatorNikolić, Dušan M.
dc.date.accessioned2018-09-06T08:39:40Z
dc.date.available2018-09-06T08:39:40Z
dc.date.available2020-07-03T09:03:10Z
dc.date.issued2018-06-28
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=5934
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/9780
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:18080/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=512160221
dc.description.abstractperiodu od 2011. do 2015. godine, u okviru 22 administrativna okruga Srbije i 136 lokaliteta gajenja paradajza, utvrđeno je značajno prisustvo virusnih zaraza paradajza. Testiranjem 3486 sakupljenih uzoraka na prisustvo 10 virusa infektivnih za paradajz, primenom DAS-ELISA testa i/ili RT-PCR uz korišćenje virusno-specifičnih prajmera, detektovano je šest virusa paradajza: virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus mozaika duvana (Tobacco mosaic virus, TMV) i virus mozaika paradajza (Tomato mosaic virus, ToMV). Testiranjem semena paradajza komercijalno dostupnih sorti i hibrida, koje je izvedeno 2012. godine DAS-ELISA metodom, na prisustvo četiri virusa koji se prenose semenom: AMV, ToMV, TMV i virusa mozaika pepina (Pepino mosaic virus, PepMV), nije utvrđeno prenošenje semenom paradajza ni jednog od ispitivanih virusa. Tokom petogodišnjeg perioda istraživanja utvrđeno je dominantno prisustvo CMV i PVY u različitim godinama istraživanja i lokalitetima gajenja paradajza. Pojedinačne infekcije su bile najznačajnije i njihova zastupljenost se kretala od 27,2% u 2014. godini do 60,7% tokom 2015. godine. Od mešanih infekcija najzastupljenije su bile dvostruke infekcije čija se zastupljenost kretala od 3% u 2014. godini do 33,7% tokom 2011. godine. Najzastupljeniju dvostruku infekciju činili su CMV i PVY čija se zastupljenost kretala od 5,8% tokom 2013. godine do 17,2% u 2011. godini. Trostruke i četvorostruke infekcije zabeležene su u manjem procentu i bile su značajne u pojedinim lokalitetima, a najviše su bile zastupljene sa 4,9% tokom 2011. godine. Detekcija i identifikacija svih šest prouzrokovača virusnih oboljenja na paradajzu u Srbiji potvrđena je RT-PCR metodom korišćenjem odgovarajućih specifičnih prajmera. Tokom istraživanja unapređena je molekularna dijagnostika svih šest virusa do nivoa vrste ili nižih taksonomskih kategorija. Determinisane su nukleotidne sekvence određenih delova genoma virusa i ustanovljen je stepen homologije dobijenih sekvenci sa sekvencama drugih izolata deponovanih u NCBI bazi podataka. Izolati CMV iz Srbije, na osnovu filogenetskih analiza sekvenci CP gena, grupisali su se u okviru podgrupa IA i II...sr
dc.description.abstractIn the period from 2011 to 2015, within the 22 administrative districts of Serbia and 136 localities of tomato cultivation, the significant presence of tomato virus infections was established. When testing 3486 collected samples for the presence of 10 viruses infectious to tomatoes by the DAS-ELISA test and/or RT-PCR using virus-specific primers, six tomato viruses were detected: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tomato spotted wilt orthotospovirus (TSWV), Alfalfa mosaic virus (AMV), Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV). In 2012, tomato seeds of commercially available varieties and hybrids were tested using the DAS-ELISA method for the presence of four transmitted viruses (AMV, ToMV, TMV and PepMV) and the results show that no tomato seed transmissions of any of the tested viruses were detected. During the five-year research period, the dominant presence of CMV and PVY in different years of research and tomato cultivation sites was determined. Individual infections were most significant and their prevalence ranged from 27.2% in 2014 to 60.7% in 2015. Regarding the mixed infections, the most commonly reported cases were double infections with a prevalence ranging from 3% in 2014 to 33.7% in 2011. The most common double infection was made by CMV and PVY, whose presence ranged from 5.8% in 2013 to 17.2% in 2011. Triple and quadruple infections were minor and they were significant in certain localities, with the highest percentage of 4.9% in 2011. The detection and identification of all six viruses causing infections on tomatoes in Serbia were confirmed by the RT-PCR method using appropriate specific primers. During the study, the molecular diagnostics of all six viruses including the level of the species or lower taxonomic categories was improved. The nucleotide sequences of certain parts of the genome of the virus were determined and the degree of homology of the obtained sequences was verified determined with the sequences of other isolates deposited in the NCBI database. Isolates of CMV from Serbia, based on phylogenetic analysis of the CP gene sequences, were grouped in subgroups IA and II. Using phylogenetic analysis, based on the sequences of P1 gene, PVY isolates originating in Serbia were classified in the subclass N Europe within the clusters of necrotic strains...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Пољопривредни факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceУниверзитет у Београдуsr
dc.subjectparadajzsr
dc.subjecttomatoen
dc.subjectvirusesen
dc.subjectDAS-ELISAen
dc.subjectmolecular detectionen
dc.subjectmolecular characterizationen
dc.subjectCMVen
dc.subjectCMV satRNAen
dc.subjectPVYen
dc.subjectTSWVen
dc.subjectvirusisr
dc.subjectDAS-ELISAsr
dc.subjectmolekularna detekcijasr
dc.subjectmolekularna karakterizacijasr
dc.subjectCMVsr
dc.subjectCMV satRNAsr
dc.subjectPVYsr
dc.subjectTSWVsr
dc.titleDiverzitet i karakterizacija virusa paradajza u Srbijisr
dc.title.alternativeDiversity and characterization of tomato viruses in Serbiaen
dc.typedoctoralThesisen
dc.rights.licenseBY-NC-ND
dcterms.abstractКрстић, Бранка; Станковић, Ивана; Делибашић, Горан; Ристић, Данијела; Моравчевић, Ђорђе; Николић, Душан М.; Диверзитет и карактеризација вируса парадајза у Србији; Диверзитет и карактеризација вируса парадајза у Србији;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/13718/Disertacija.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/13719/IzvestajKomisije17464.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/13718/Disertacija.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/13719/IzvestajKomisije17464.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_9780


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