Ispitivanje mogućnosti formiranja biofilma u uslovima in vitro kod različitih serovarijeteta Salmonella vrsta izolovanih iz uzoraka hrane za životinje
Analysis of possible biofilm formation from different Salmonella serovars isolated from animal food in in vitro conditions.
Author
Prunić, Bojana Z.Mentor
Mišić, Dušan
Committee members
Milanov, Dubravka
Radojičić, Sonja

Marković, Radmila

Bulajić, Snežana

Metadata
Show full item recordAbstract
Cilj ovog istraživanja je bio ispitivanje sposobnosti formiranja biofilma kod
serovarijeteta Salmonella izolovanih iz hrane za životinje, poreklom iz 18 mešaona
stočne hrane na području Južnobačkog okruga. Izolati korišćeni u ispitivanju (n=100)
izolovani su poreklom iz uzoraka hrane za životinje uzorkovanih tokom dve godine
ispitivanja (period 2012-2014). Identifikacija izolata izvršena je na osnovu kulturelnih,
biohemijskih i seroloških karakteristika na osnovu preporuka standarda SRPS EN ISO
6579:2008. Potvrda i serološka tipizacija izolata Salmonella izvedena je u Nacionalnoj
referentnoj laboratoriji za Salmonella, Shigela, Vibrio cholerae i Yersinia enterocolitica,
Instituta za javno zdravlje Srbije „Dr Milan Jovanović Batut“ u Beogradu. Kao
kontrolni sojevi, u ispitivanju su korišćene referentne kulture S. Typhimurium ATCC
14028 i S. Enteritidis ATCC 13076.
Kao supstrat za formiranje biofilma korišćene su površine od polistirena i
nerđajućeg čelika. Sposobnost formiranja biofilma... ispitana je primenom sledećih
metoda: Congo red agar test, pelikula test, test na mikrotitracionim pločama upotrebom
kristal violet boje, i skenirajuća elektronska mikroskopija (SEM). Sva ispitivanja
izvedena su upotrebom dve hranljive podloge (tripton soja bujona i Luria Bertani
bujona) pri temperaturi inkubacije od 20C i 37C.
Iz hrane za životinje izolovani su sledeći serovarijeteti Salmonella: Tennessee
(18%), Agona (9%), Montevideo (15%), Enteritidis (12%), Stanleyville (6%), Infantis
(12%), Typhimurium (3%), Typhimurium monofazni (1%), Mbandaka (6%),
Senftenberg (7%), Jerusalem (2%), Thompson (1%), Amsterdam (1%), Colindale (1%),
Dahra (1%), Livingstone (1%). Kod četiri izolata nije ustanovljen serovarijetet
(Salmonella enterica subspecies enterica (1,3,19:i:-)).
Prema rezultatima Congo red agar testa, na temperaturi od 20C, 98% izolata
Salmonella ekspresioniralo je jednu ili obe komponente matriksa biofilma i formiralo
kolonije RDAR, PDAR i BDAR morfotipova...
The aim of this research was the investigation into the biofilm-forming ability of
Salmonella serovars isolated from animal feed samples originating from 18 feedproducing
facilities located in South Bačka District. The isolates which were tested
(n=100) were isolated from feed samples collected in a two-year period (from 2012 to
2014). The identification of the isolates was made based on the cultural, biochemical
and serological characteristics, following the recommendations given in the SRPS EN
ISO 6579:2008 standard. The conformation and serological typing of Salmonella
isolates were done in the National Reference Laboratory for Salmonella, Shigela, Vibrio
cholerae and Yersinia enterocolitica, at the Institute of Public Health of Serbia 'Dr
Milan Jovanović Batut' in Belgrade. In this study reference cultures of S. Typhimurium
ATCC 14028 and S. Enteritidis ATCC 13076 were used as the control strains.
Surfaces made of polystyrene and of stainless steel were used as substrates for
biofi...lm formation. The ability of Salmonella to form biofilm was tested with the
following methods: Congo red agar test, pellicle test, microtiter plate assay with the use
of crystal violet dye, and scanning electron microscopy (SEM). The whole investigation
was carried out at two temperatures of incubation (20C and 37C).
The following Salmonella serovars were isolated from animal feed: Tennessee
(18%), Agona (9%), Montevideo (15%), Enteritidis (12%), Stanleyville (6%), Infantis
(12%), Typhimurium (3%), Typhimurium monofazni (1%), Mbandaka (6%),
Senftenberg (7%), Jerusalem (2%), Thompson (1%), Amsterdam (1%), Colindale (1%),
Dahra (1%), Livingstone (1%). In four isolates serovars were not determined
(Salmonella enterica subspecies enterica (1,3,19:i:-)).
According to the results of Congo red agar test, when grown at 20C, 98% of the
isolates expressed one or both components of the Salmonella biofilm matrix and formed
colonies of various morphotypes (RDAR, PDAR and BDAR)...
Faculty:
Универзитет у Београду, Факултет ветеринарске медицинеDate:
06-12-2017Projects:
- Wild animal health monitoring and introduction of new biotechnology procedures in detection of infectious and zoonotic agents - risk analysis for human health, domestic and wild animal health and for environmental contamination (RS-31084)
- Kontrola biohazardnih agenasa na farmama i primena biotehnoloških postupaka s ciljem da se redukuju rizici u sistemu proizvodnje bezbedne hrane i obezbedi neškodljivo uklanjanje animalnog otpada (RS-20133)