Ispitivanje antioksidativne i prooksidativne aktivnosti odabranih jedinjenja fenolnog tipa
Author
Đorović, JelenaMentor
Petrović, ZoricaCommittee members
Marković, ZoranAmić, Dragan
Manojlović, Nedeljko
Petrović, Vladimir
Metadata
Show full item recordAbstract
Izvod:
Mnoga prirodna polifenolna jedinjenja se smatraju dobrim
antioksidantima. Od kada je uočeno da igraju ključnu ulogu u odbrambenom
mehanizmu bioloških sistema, njima se pridaje još veći značaj. Iako se u
prirodi može naći širok spektar jedinjenja ovog tipa, zbog različitih ljudskih
potreba javlja se sve veće interesovanje za specifičnijim antioksidantima
fenolnog tipa, zbog čega se na njihovoj sintezi danas intenzivno radi. Međutim,
iako se pokazalo da sintetički antioksidanti pokazuju izuzetne osobine i imaju
značajnu primenu (npr. u prehrambenoj industriji), mora se voditi računa o
njihovom potencijalnom štetnom efektu.
U poslednjoj dekadi se značajna pažnja posvećuje rasvetljavanju
dualističkog antioksidativno-prooksidativnog ponašanja prirodnih i
sintetičkih fenolnih i polifenolnih jedinjenja. Naime, pokazalo se da se pod
određenim in vitro uslovima neka fenolna i polifenolna jedinjenja, ponašaju kao
dobri antioksidanti, dok se u ćeliji, odnosno normalnom biološ...kom okruženju,
ponašaju na drugačiji način. U ćelijskoj sredini ova jedinjenja, najčešće,
ispoljavaju prooksidativno dejstvo, tj. indukuju hiperprodukciju aktivnih
kiseoničnih vrsta, pre svega superoksid anjon radikala.
U okviru ove disertacije ispitane su antioksidativne i prooksidativne
osobine fenolnih jedinjenja. Kako bi se ispitali mehanizmi antiradikalskog
delovanja prirodnih jedinjenja fenolnog tipa urađena je analiza galne kiseline
kao njihovog predstavnika. Takođe, metodama teorije funkcionala gustine
ispitana je antiradikalska aktivnost sintetisanih Šifovih baza. U cilju
eksperimentalnog ispitivanja antioksidativne aktivnosti dobijenih fenolnih
jedinjenja (Šifovih baza) korišćeni su DPPH test i inhibicija enzima
lipoksigenaza, dok je njihova prooksidativna aktivnost testirana in vitro na
ćelijskim linijama primarnog kancera debelog creva i sekundarnog kancera dojke,
kao i na zdravim ćelijama MRC-5.
Summary:
Many natural polyphenolic compounds are considered to be good antioxidants.
Since it has been noticed that they play an important role in the defense mechanism of
biological systems, they are given even greater significance. Although a wide range of
compounds of this type can be found in nature, because of different human needs, there is
an increasing interest in synthesis of more specific antioxidants of the phenolic type,
which is why their synthesis is in focus these days. However, although synthetic
antioxidants have been shown to exhibit exceptional traits and have significant
application (e.g. in the food industry), their potentially harmful effects must be taken into
account.
In the last decade, considerable attention was devoted to illumination of dualistic
antioxidative-proxidative behavior of natural and synthetic phenolic and polyphenolic
compounds. Namely, certain in vitro conditions have shown that some phenolic and
polyphenolic compounds behave as go...od antioxidants, whereas they behave in a different
way in a cell or a natural biological environment. Most frequently, in the cellular
environment these compounds exhibit a prooxidative effect, i.e. induce hyperproduction
of active oxygen species, primarily superoxide anion radicals.
Within this dissertation the antioxidative and protoxidative properties of phenolic
compounds were examined. In order to investigate mechanisms of antiradical action of
natural compounds of the phenolic type, analysis of gallic acid as their representative was
performed. Also, the methods of density functional theory (DFT) were applied for the
investigation of the antiradical activity of the synthesized Schiff bases. For the purpose of
experimental testing of the antioxidant activity of the obtained phenolic compounds
(Schiff bases), DPPH and lipoxygenase enzyme inhibition were used, while their
prooxidative activity was tested in vitro on the cell lines of the primary colon cancer and
secondary breast cancer, as well as on healthy MRC-5 cells .
The obtained results are of scientific importance because they contribute to the
clarification of the antioxidative and/or antiradical behavior of phenolic compounds and
their in vitro tested prooxidative or cytotoxic action.
Faculty:
Универзитет у Крагујевцу, Природно-математички факултетDate:
27-12-2017Projects:
- Preclinical investigation of bioactive substances (RS-41010)
- Dynamics of nonlinear physicochemical and biochemical systems with modeling and predicting of their behavior under nonequilibrium conditions (RS-172015)
- Synthesis, modeling, physicochemical and biological properties of organic compounds and related metal complexes (RS-172016)
- Multiscale Methods and Their Applicatios in Nanomedicine (RS-174028)