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Effect of diet enriched with OMEGA-3 and OMEGA-6 fatty acids on myocardial function and oxido-inflammatory parameters in the heart of old rats

dc.contributor.advisorŽivković, Vladimir
dc.contributor.otherĐonović, Nela
dc.contributor.otherJakovljević, Vladimir
dc.contributor.otherArsić, Aleksandra
dc.creatorRadoman, Kristina
dc.date.accessioned2018-02-26T16:44:53Z
dc.date.available2018-02-26T16:44:53Z
dc.date.available2020-07-03T15:19:56Z
dc.date.issued2018-01-18
dc.identifier.urihttp://eteze.kg.ac.rs/application/showtheses?thesesId=5555
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/9197
dc.identifier.urihttps://fedorakg.kg.ac.rs/fedora/get/o:912/bdef:Content/download
dc.description.abstractUvod: Rezultati istraživanja daju originalan i važan doprinos sagledavanju pozitivnih i nagativnih efekata polinezasićenih masnih kiselina zastupljenih u ishrani, kao i mogućnostima i potrebama izmene njihovog odnosa u cilju postizanja povoljnih efekata na kardiovaskularni sisteam. Rezultati istraživanja imaju veliki praktični značaj, pre svega imajući u vidu da se radi o ispitivanju organskog sistema čiji poremećaji zahvataju daleko najveći postotak svetske populacije. Cilj: Cilj ovog istraživanja je bilo ispitivanje hroničnih efekata primene ishrane obogaćene omega-3 i omega-6 masnim kiselinama na funkciju miokarda, koronarni protok i oksido-inflamacijske parametre starih pacova. Materijal i metode: Istraživanje je dizajnirano kao eksperimentalna studija sprovedena ex vivo. Nakon hroničnog tretmana od 6 nedelja odgovarajućom ishranom, životinje su se žrtvovale kako bi se sprovela istraživanja na izolovanom srcu i biohemijske analize iz uzoraka perfuzata, plazme i lizata eritrocita. Biohemijske analize su određivane u uzorcima koronarnog venskog efluenta, koji je sakupljan na kraju kontrolnog perioda i na kraju perioda aplikacije ispitivane supstance. Svi reaktivni molekuli, koji su bili od interesa za naše istraživanje su mereni spektrofotometrijskom metodom. Pored toga, u uzorcima plazme i lizatu eritrocita merile su se vrednosti pro-oksidacionih, antioksidacionih i inflamatornih parametara. Rezultati: Hronična primena suplementacije alfa-linoleinskom kiselinom ima kardioprotektivno dejstvo u odnosu na primenu linolne kiseline na modelu izolovanog srca pacova. Ishrana obogaćena omega-6 masnim kiselinama utiče negativno na funkciju miokarda izolovanog srca pacova u odnosu na ishranu obogaćenu omega-3 masnim kiselinama. Primenom hronične ishrane sa odnosom omega-3/omega-6 u našem istraživanju 1:3,3 (55%:17%) u korist omega-3 masnih kiselina u vidu ulja lanenog semena, kao i primena masnih kiselina u odnosu omega-3/omega-6 1:9 (9%:73%) u vidu ulja noćurka u korist omega-6 masnih kiselina, pokazali smo značajne razlike u pogledu efekta na fukciju miokarda i redoks status pacova, sa naglaskom na kardiprotektivno delovanje odnosa 1:3,3. Mehanizmi kojima linoleinska kiselina ostvaruje kardiprotektivno dejstvo oslanjaju se na sniženje indeksa lipidne peroksidacije, vodonik peroksida kao povećanje aktivnosti katalaze, superoksid dismutaze iz intaktnu koronarnu cirkulaciju. Zaključak: Nalazi sadašnjeg istraživanja mogu da pomognu u boljem razumevanju uticaja omega-3 i omega-6 masnih kiselina na održavanje kardiovaskularne homeostaze. ovi rezultati su od velikog praktičnog značaja, s obzirom a široku primenu suplemenata koji se sastoje od ovih jedinjenja.sr
dc.description.abstractIntroduction: The results of the research give an original and important contribution to the examination of the positive and negative effects of polyunsaturated fatty acids present in the diet, as well as the possibilities and needs of changing their relationship in order to achieve favorable effects on the cardiovascular system. The results of the research have a great practical significance, predominantly having in mind that this disruptions of this organic system are by far the largest percentage of the world population. Aim: The aim of this study was to examine the chronic effects of diet enriched with omega-3 and omega-6 fatty acids on myocardial function, coronary flow and oxidizing inflammatory parameters of old rats. Material and methods: The study was designed as an ex vivo experimental study. After 6 weeks of chronic treatment with adequate nutrition, animals were sacrificed to perform isolated heart investigation and biochemical analysis from perfusate, plasma, and erythrocyte lysates. Biochemical analyzes were determined in samples of the coronary venous effluent, which was collected at the end of the control period and at the end of the period of application of the tested substance. All reactive molecules that were of interest to our research were measured by a spectrophotometric method. In addition, in the plasma and lysate erythrocyte samples, the values of pro-oxidation, antioxidant and inflammatory parameters were measured. Results: The alpha-linoleic acid supplementation has a stronger cardioprotective effect than linoleic acid on the model of the isolated rat heart. Nutrition enriched with omega-6 fatty acids more affects the myocardial function of the isolated heart of the rat in relation to than diet enriched with omega-3 fatty acids. Using chronic nutrition with omega-3 / omega-6 ratio 1:3.3 (55%: 17%) in favor of omega-3 fatty acids in the form of flax seed oil, and the application of fatty acids in the ratio of omega-3 / omega-6 1:9 (95:73%) in the form of omega-6 fatty acid oil, we showed significant differences in the effect on myocardial function and rat's redox status, with an emphasis on cardioprotective ratio 1:3,3. Mechanisms through which linoleic acid achieves cardioprotective activity rely on the reduction of the lipid peroxidation index, hydrogen peroxide and increase in catalase activity and superoxide dismutase with intact coronary circulation. Conclusions: Findings of present study may help in better understanding of the inluence of omega-3 and omega-6 fatty acids on the maintenance of cardiovascular homeostasis. Taken into consideration wide usage of supplements consisting of these compounds, these results are of great practical significance.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Крагујевцу, Факултет медицинских наукаsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Крагујевцуsr
dc.subjectoksidacioni stressr
dc.subjectomega-3 fatty acidsen
dc.subjectizolovano srce pacovasr
dc.subjectomega-6 fatty acidsen
dc.subjectoxidative stressen
dc.subjectisolated heart ratsen
dc.titleUticaj ishrane obogaćene OMEGA-3 i OMEGA-6 masnim kiselinama na funkciju miokarda i oksidativno-inflamacijske parametre kod srca starih pacovasr
dc.title.alternativeEffect of diet enriched with OMEGA-3 and OMEGA-6 fatty acids on myocardial function and oxido-inflammatory parameters in the heart of old ratsen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dcterms.abstractЖивковић, Владимир; Ђоновић, Нела; Јаковљевић, Владимир; Aрсић, Aлександра; Радоман, Кристина; Утицај исхране обогаћене ОМЕГA-3 и ОМЕГA-6 масним киселинама на функцију миокарда и оксидативно-инфламацијске параметре код срца старих пацова; Утицај исхране обогаћене ОМЕГA-3 и ОМЕГA-6 масним киселинама на функцију миокарда и оксидативно-инфламацијске параметре код срца старих пацова;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/50049/Disertacija.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/50050/Izvestaj_Kristina_Radoman_Medicinski.pdf


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