Uticaj smanjene funkcije štitaste žlezde majki na razvoj jajnika mladunaca pacova u ranom postnatalnom periodu
Impact of reduced thyroid function of mothers on development of rat offspring ovaries in the early postnatal period
Докторанд
Danilović-Luković, JelenaМентор
Korać, Aleksandra![](/themes/MirageNardus/images/orcid.png)
Чланови комисије
Radovanović, Anita![](/themes/MirageNardus/images/orcid.png)
Čakić-Milošević, Maja
![](/themes/MirageNardus/images/orcid.png)
Kovačević-Filipović, Milica
![](/themes/MirageNardus/images/orcid.png)
Метаподаци
Приказ свих података о дисертацијиСажетак
Tiroidni hormoni (TH) su od ključnog značaja za razviće sisara u prenatalnom periodu.
Prethodne studije pokazuju da subklinički hipotiroidizam ima za posledicu povećanu stopu
atrezije u jajnicima mladunaca u prepubertalnom i pubertalnom uzrastu. Uticaj smanjene
koncentracije TH na ranu postnatalnu folikulogenezu i razviće jajnika nije do sada ispitano. Ova
disertacija ima za predmet istraživanja uticaj hipotiroidizma majki indukovanog 6-n-propil-2-
tiouracilom (PTU) na folikulogenezu mladunaca pacova (n=15) 0, 4. i 7. dana po rođenju. Kod
mladunaca 0. dana određivani su broj karakteristike oogonija i oocita normalne i izmenjene
morfologije svetlosnom i elektronskom mikroskopijom. Pomenutim metodima kod mladunaca 4.
i 7. dana starosti u jajnicima je određivan broj nestova, oogonija i oocita po nestu,
primordijalnih, primarnih, sekundarnih i preantralnih folikula. Prisustvo glikogena i
mukopolisaharida u tkivu jajnika mladunaca 0. dana određivano je PAS (eng. Periodic Acid
Shiff) histohe...mijskim bojenjem, a prisustvo kalcijuma u tkivu jajnika pacova 4. i 7. dana Alizarin
Red bojenjem. Volumenska gustina nukleusa, mitohondrija i glatkog endoplazmatičnog
retikuluma u oocitama primordijalnih folikula analizirana je u jajnicima pacova 4. i 7. dana
starosti. Imunohistohemijskom metodom detektovana je imunoekspresija PCNA (eng.
proliferating cell nuclear antigen) kao markera proliferacije, dva markera apoptoze (kaspaza 3 i
TUNEL (eng. terminaldeoxynucleotidyl transferase dUTP nick end labelling)) kao i dva markera
autofagijе (ATG7 i LC3I/II). Hipotiroidni pacovi na rođenju imali su smanjen broj oogonija u
mitozi i u mirovanju, kao i povećan broj atipičnih oogonija/oocita. Na ultrastukturnom nivou
pokazano je prisustvo degenerisanih ćelija u pahitenu (Z-ćelije), oogonija/oocita u autofagiji,
apoptozi i kombinaciji ova dva tipa ćelijske smrti, kod obe grupe životinja ovog uzrasta.
Najbrojniji tipovi atipičnih ćelija kod hipotiroidnih pacova 0. dana su bile oogonije/oocite u
autofagiji i Z-ćelije. U prilog ubrzanoj maturaciji neizmenjenih oogonija/oocita kod ove grupe
životinja ide zapaženo prisustvo prominentnih organela i ekspresija PCNA. Broj TUNEL
pozitivnih oogonija/oocita je bio manji kod hipotiroidnih pacova 0. dana, dok nije bilo razlike
kada je u pitanju imunoekspresija kaspaze 3, ATG7 i LC3. Kod hipotiroidnih pacova starih 4
dana smanjen je broj oogonija i oocita po nestu, primarnih i sekundarnih folikula, a povećan je
broj primordijalnih folikula. Kod hipotiroidnih pacova starih 7 dana prisutan je veći broj
primarnih, sekundarnih i preantralnih folikula. Alizarin Red bojenje i elektronska mikroskopija
su ukazali na prisustvo prominentnog endoplazminog retikuluma kod hipotiroidnih pacova starih
4 dana...
Thyroid hormones (TH) are essential for mammals’ development in the prenatal period. Previous
studies have shown that subclinical hypothyroidism results in an increased rate of atresia in the
pups ovaries in prepubertal and pubertal age. The impact of reduced concentration of TH on the
early postnatal folliculogenesis and development of ovarian cancer has not been studied until
now. The aim of the current thesis is to explain the influence of maternal hypothyroidism
induced by 6-n-propyl-2-thiouracil (PTU) on folliculogenesis of rat offspring (n = 15) of at the
rat at the day of birth (0. day) and 4 and 7 days old. The number and morphological
characteristics of oogonia and oocytes of normal and altered morphology was determined using
light and electron microscopy. These methods were applied to determine the number of germ cell
nests, oogonia and oocytes within nest, primordial, primary, secondary and preantral follicles in
the ovaries of 4 and 7 days old pups. The presence of glycogen... and mucopolysaccharides in the
ovary tissue of the pups at the 0. day was detected using PAS (Periodic Acid Shiff)
histochemical staining and the presence of calcium by Alizarin Red staining in the ovary tissue
of 4 and 7 days old pups. The volume density of nucleus, mitochondria and smooth endoplasmic
reticulum in oocytes of primordial follicles was obtained in the ovaries 4 and 7 days old rats.
Immunohistochemistry was used to detect the expression of PCNA (proliferating cell nuclear
antigen), as a marker of proliferation, two markers of apoptosis (caspase-3 and TUNEL (terminal
deoxynucleotidyl transferase dUTP nick end labeling)) as well as two markers for autophagy
(ATG7 LC3I / II). Hypothyroid rats at birth had reduced number of resting oogonia and oogonia
undergoing mitosis, as well as the increased number of atypical oogonia/oocytes. Ultrastructural
analyses showed the presence of degenerated cells in pachytene (Z-cell), oogonia/oocytes in
autophagy, apoptosis and the combination of these two types of cell death in both groups of
animals of this age. The most common types of atypical cells in hypothyroid rats at birth were
oogonia/oocytes autophagy and Z-cell. In addition to the accelerated maturation morphologically
unchanged oogonia/oocytes in this group of animals was the finding of the presence of
prominent organelles in oocyte cytoplasm, followed with the increased expression of PCNA.
Number of oogonia/oocytes labeled with TUNEL was lower in hypothyroid rats at birth, while
there was no difference when it comes to expression of caspase-3, ATG7 and LC3...
Факултет:
Универзитет у Београду, Биолошки факултетДатум одбране:
31-03-2017Пројекти:
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)