Semenke lana, susama i golice i njihovi ekstrakti, ferulinska kiselina i enterolignani: efekti na lipidni status kod osoba na hemodijalizi, vezivanje za humani serum-albumin in vitro i uticaj na reaktivnost Cys34 tiolne grupe
Seeds of flax, sesame and pumpkin and their extracts, ferulic acid and enterolignans: effects on the lipid status in patients on hemodialysis, binding to human serum albumin in vitro and influence on Cys34 thiol group reactivity
Докторанд
Takić, Marija M.Ментор
Mandić, LjubaЧланови комисије
Ristić-Medić, DanijelaMandić, Ljuba
Nikolić, Milan
Jovanović, Vesna
Метаподаци
Приказ свих података о дисертацијиСажетак
Poznato je da su semenke lana, susama i golice bogate esencijalnim masnim kiselinama i
da predstavljaju značajni dijetarni izvor vitamina E i polifenola. Kod osoba sa hroničnom
bubrežnom insuficijencijom, na programu hemodijalize, aktuelna su ispitivanja dijetarnih mera u
cilju smanjenja incidence kardiovaskularnih oboljenja. Iz navedenog proizišao je cilj ove
disertacije da se ispitaju efekti suplementacije ishrane smešom semenki lana, susama i golice kod
bolesnika na hemodijalizi.
Antioksidativna uloga humanog serum-albumina (HSA) u značajanoj meri određuje
ukupnu antioksidativnu aktivnost u plazmi, a analiza HSA pokazala je da je kod bolesnika na
programu hemodijalize smanjen sadržaj redukovane Cys34 tiolne grupe (od koje potiče
antioksidativni potencijal HSA) i povećan stepen karbonilacije HSA. Stoga je drugi cilj ove
disertacije bio da se in vitro ispita uticaj vezivanja komponenti ekstrakta smeše semenki lana,
susama i golice, ferulinske kiseline (FE) i metabolita lignana (entero...laktona (EL) i enterodiola
(ED), koji se povećavaju u cirkulaciji nakon konzumiranja namirnica bogatih biljnim lignanima),
kao i masnih kiselina, na promenu konformacije HSA i reaktivnosti (antioksidativnog
potencijala) Cys34 tiolne grupe, a da bi se sagledali mogući mehanizmi kojima komponente lana,
susama i golice ostvaruju svoje in vivo efekte, pre svega antioksidativno dejstvo.
Analizom masnokiselinskih profila ukupnih lipidnih ekstrakata smeše semenki gasnotečnom
hromatografijom potvrđeno da je u semenkama lana najzastupljenija α-linolenska
kiselina (ALA), a u semenkama susama i golice linolna kiselina (LA). Hladnoceđena ulja
ispoljila su različitu antioksidativnu aktivnost (rafinisano suncokretovo ulje>maslinovo ulje>ulje
lana>ulje golice> ulje susama) pri DPPH testu, u opsegu od 970 do 2000 μmola TE/1000 g ulja.
Sadržaj hidrofilnih antioksidanasa u hladnoceđenim uljima je veoma nizak, sem za hladoceđeno
maslinovo ulje. Pored lipofilnih antioksidanasa, pokazano je da metanolni ekstrakti
obezmašćenih semenki (posebno lana) sadrže polifenole...
It is known that seeds of flax, sesame, and pumpkin are reach in essential fatty acids, and
represent significant dietary source of vitamin E and polyphenols. In patients with chronic renal
insufficiency, on hemodialysis treatment, research topics about dietary supplementations with
aims to reduce incidence of cardiovascular diseases, are actual. Therefore, the goal of this
dissertation was to investigate the effects of diet supplementation with the mixture of flax,
sesame, and pumpkin seeds in patients on hemodialysis.
The total antioxidative activity in plasma is significantly determined by antioxidative role
of human serum albumin (HSA), and analysis of the HSA in patients on hemodialysis showed
that the content of the free Cys34 thiol group (from which the HSA antioxidative potential
originates) is reduced and on the other hand, degree of HSA carbonylation is increased. Thus, the
second goal of this dissertation was in vitro investigation of the binding of flax, sesame, and
pumpkin...s seeds extracts components, ferulic acid (FE) and lignan metabolites (enterolactone
(EL), enterodiol (ED), which are increased in circulation after consumption of food reach with
plants lignan), as well as fatty acids, on the conformational change of the HSA and on the
reactivity (antioxidative potential) of Cys34 thiol group, with the final goal to perceive the
possible mechanisms with which the flax, sesame, and pumpkin seeds explore in vivo
antioxidative effects.
Analysis of fatty acids profiles of total lipid extracts of seeds mixture using gas-liquid
chromatography, confirmed that flaxseeds are the most abundant with α-linolenic acid (ALA),
and sesame and pumpkin with linoleic acid (LA). Cold pressed oils showed different
antioxidative activity (refined sunflower oil>olive oil>flaxseed oil>pumpkin oil>sesame oil) in
the DPPH test (ranged from 970 to 2000 μmol TE/1000g oil). Content of hydrophilic
antioxidants in cold pressed oils was extremely low, except for cold pressed olive oil. Besides
lipophilic antioxidants, it was shown that methanol extracts of defatted seeds (particularly
flaxseed) contain polyphenols. Antioxidative activity of the extracts (flax>sesame>pumpkin
seeds), determined using DPPH and ABTS tests, was corresponded to the contents of total
phenols in extracts...
Факултет:
Универзитет у Београду, Хемијски факултетДатум одбране:
13-09-2016Кључне речи:
lan / flaxseed / sesame / pumpkin seed / n-3 fatty acids / n-6 fatty acids / ferulic acid / enterodiol / enterolactone / hemodialysis / human serum albumin / antioxidative properties of the human serum albumin / Cys34 thiol group reactivity / susam / golica / n-3 masne kiseline / n-6 masne kiseline / ferulinska kiselina / enterodiol / enterolakton / hemodijaliza / humani serum-albumin / antioksidativna aktivnost humanog-serum albumina / reaktivnost Cys34 tiolne grupeRelated items
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