Analiza frekvencije hromozomskih aberacija u zaposlenih na različitim radnim mestima u zoni jonizujućeg zračenja
The analysis of the chromosomal aberrations frequency of the employees from different workplaces in the zone of ionizing radiation.
Author
Đoković-Davidović, Jelena Đ.Mentor
Milovanović, Aleksandar P. S.Committee members
Bulat, Petar
Artiko, Vera
Mačvanin, Nada
Metadata
Show full item recordAbstract
Cilj predstavljenog istraživanja je da ispita uticaj malih doza jonizujućeg zračenja
na zaposlene na različitim radnim mestima u zoni jonizujućeg zračenja.
Materijal i metode: Za studiju retrospektivne prirode analizirani su rezultati
dobijeni iz kartona preventivno-periodičnih medicinskih pregleda 148 zaposlenih na
različitim radnim mestima u zoni jonizujućeg zračenja u Kliničkom Centru Srbije a koji
obavljaju preglede u Institutu za medicinu rada Srbije.
Ispitanici su podeljeni u tri velike grupe u odnosu na izvor zračenja-oblast rada na
zaposlene u nuklearnoj medicini, intrerventnoj radiologiji i opštoj radiologiji. Podaci
korišćeni za studiju su podaci sa prethodnog/prvog periodičnog pregleda koji je obavljan
pre ulaska u zonu jonizujućeg zračenja ili u toku prve godine izlaganja (u toku obavljanja
specijalizacije, u skladu sa odlukom poslodavca da pošalje zaposlenog na pregled) i
podaci sa poslednjeg periodičnog pregleda.
Uzimani su u obzir ukupni i ekspozicioni radni staž, vrsta ...izvora zračenja, hematološki
parametri (eritrociti, retikulociti, leukociti sa leukocitarnom formulom, trombociti),
frekvenca i broj nestabilnih hromozomskih aberacija (ishod testa nestabilnih
hromozomskih aberacija) kao i ishod mikronukleusnog testa i njihov broj (značajnih za
ocenu radne sposobnosti), primljena kumulativna petogodišnja doza zračenja, morbiditet
ispitanika i prisutvo profesionalnih bolesti.
Hematološki parametri (eritrociti-Er, retikulociti-Rtc, ukupni leukociti-Leu, monociti-Mo,
neutrofili-Neut, eozinofili-Eo, bazofili-Ba, limfociti-Ly i trombociti-Tr) su dobijani
ispitivanjem periferne venske krvi u aparatu Beckman Coulter HMX ( na poslednjem
periodičnom pregledu) a na prethodnom su uglavnom ispitivani starom metodom ručnog
brojanja ćelija (preračunatih za statistički obradu).
Nestabilne hromozomske aberacije su dobijane modifikovanom Moorhead’s mikro
metodom.
Mikronukleusni test je rađen metodom po Fenech-u i Morley-u...
The aim of the research is to analyse the influence of the small doses of ionizing
radiation on the employees from different workplaces in the zone of ionizing radiation.
Material and methods: For this study of the retrospective nature we have analyzed
results obtained from the medical records based on preventive-periodical examinations of
148 persons employed at different workplaces in the zone of ionizing radiation at the
Clinical Center of the Republic of Serbia. All of the employees go to medical
examinations at the Institute of Occupational Medicine of Serbia. The employees were
divided into three large groups as related to the source of radiation – the field of work: the
nuclear medicine employees, the interventional radiology employees and the general
radiology employees. The data was taken from the previous/initial checkup which had
been done before the entrance to the zone of ionizing radiation or during the first year of
exposure to radiation (during the specialization period..., in accordance with employer’s
decision to send an employee to the examination) as well as the data from the last
periodical examination.
We also took into consideration total and exposition work periods, type of radiation
source, haematological parameters (erythrocytes, reticulocytes, leukocytes with
differential blood count, platelets), frequency and number of unstable chromosomal
aberrations (the results of the test of the unstable chromosomal aberrations), the results of
micronucleus test and their number (important for the assessment of working ability), as
well as received cumulative five-year radiation dose, morbidity of patients and presence of
occupational diseases.
The hematological parameters (erythrocytes-RBC, reticulocytes-Rtc, total leucocytes-
WBS, monocytes-Mo, neutrophils-Neu, eosinophils-Eo, basophils-Ba and platelets) were
obtained through processing of the peripheral vein blood by Beckman Coulter HMX
apparatus (at the periodical checkup), while at the previous / initial checkup it was done
through the old method of manual cell counting (recalculated for statistical processing)...