Uticaj odabranih monoterpena na aktivnost dijagnostički značajnijih enzima u kontrolnom humanom serumu in vitro

Abstract

In order to determine the terpenes effect on enzyme activity is been studied the impact of aqueous (prepared manually (I1) or ultrasound (I2) mixing) and DMSO (dimethyl sulfoxide) terpenes solution (carvacrol, linalool, nerol and geraniol) on the activity of creatine kinase, lactate dehydrogenase, alkaline phosphatase, gamma-glutamyl transferase, aspartate aminotransferase and alanine aminotransferase,as well as the impact of the same type of terpenes solution (carvacrol, linalool, nerol, geraniol, β-pinene, myrcene, citral, eucalyptol, terpineol, and eugenol) on the activity of amylase in the control sera (with the normal (N) and increased (H) activity of the ingredients. Measurement of activity was conducted after incubation of the serum sample from the 10 minutes and aqueous solutions of terpenes prepared as I1 or as I2, or solutions of terpenes in DMSO at four concentrations. All the terpenes solutions have demonstrated that the inhibitory effect depends on various parameters: kinds of serum (higher percentage of inhibition in N than the H serum), the type of solvent ( the aqueous solution as I2 and DMSO solutions showed approximately the same percentage of inhibition in both types of serum) and way of preparing the aqueous solutions (a higher percentage of inhibition have the aqueous solution of terpenes as I2 in N serum and as I1 in H serum), as well as on the type of terpenes or enzymes. Statistical data processing all terpenes solution (with the partial exception of neroli and geraniol) confirmed the grouping or by type of serum or by type of solvent or the method of preparation of aqueous solution.