Uticaj odabranih monoterpena na aktivnost dijagnostički značajnijih enzima u kontrolnom humanom serumu in vitro
Author
Jelenković, Ljiljana A.Mentor
Palić, IvanCommittee members
Stankov Jovanović, Vesna
Mitić, Violeta
Radulović, Milanka
Jevtović Stoimenov, Tatjana

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In order to determine the terpenes effect on enzyme activity is been studied
the impact of aqueous (prepared manually (I1) or ultrasound (I2) mixing) and
DMSO (dimethyl sulfoxide) terpenes solution (carvacrol, linalool, nerol and
geraniol) on the activity of creatine kinase, lactate dehydrogenase, alkaline
phosphatase, gamma-glutamyl transferase, aspartate aminotransferase and
alanine aminotransferase,as well as the impact of the same type of terpenes
solution (carvacrol, linalool, nerol, geraniol, β-pinene, myrcene, citral,
eucalyptol, terpineol, and eugenol) on the activity of amylase in the control
sera (with the normal (N) and increased (H) activity of the ingredients.
Measurement of activity was conducted after incubation of the serum sample
from the 10 minutes and aqueous solutions of terpenes prepared as I1 or as
I2, or solutions of terpenes in DMSO at four concentrations. All the terpenes
solutions have demonstrated that the inhibitory effect depends on various
par...ameters: kinds of serum (higher percentage of inhibition in N than the H
serum), the type of solvent ( the aqueous solution as I2 and DMSO solutions
showed approximately the same percentage of inhibition in both types of
serum) and way of preparing the aqueous solutions (a higher percentage of
inhibition have the aqueous solution of terpenes as I2 in N serum and as I1 in
H serum), as well as on the type of terpenes or enzymes. Statistical data
processing all terpenes solution (with the partial exception of neroli and
geraniol) confirmed the grouping or by type of serum or by type of solvent or
the method of preparation of aqueous solution.