Uloga bakteriofaga familije Siphoviridae u lizogenoj konverziji vrste Bordetella bronchiseptica i njihov antimikrobni potencijal

Abstract

U ovom radu su izolovani specifični Bordetella bronchiseptica bakteriofagi iz iz priodne sredine koji pripadaju familiji Siphoviridae. Bakteriofagi su okarakterisani u cilju razmatranja njihove uloge u lizogenoj konverziji bakterije i mogućnosti primene u kontroli vrste B. bronchiseptica. U tu svrhu ispitivane su morfološke karakteristike odabranih faga, litički spektar faga, i karakteristike genoma i proteina faga. Takođe, ispitivana je i stabilnost faga u različitim uslovima sredine, njihova litička efikasnost i efekat na formiranje i vec formirani biofilm. U radu je ispitana i uloga faga u produkciji biofilma, rezistenciji na antibiotike, procesu hemolize i pokretljivosti kod vrste B. bronchiseptica. Rezultati ovog rada jasno ukazuju na ulogu faga u lizogenoj konverziji vrste B. bronchiseptica kao i mogućnost njihove primene, uz određene modifikacije, kao anti-B.bronchiseptica agenasa.

In this paper Bordetella bronchiseptica specific phages belonging to family Siphoviridae were isolated from environment. Bacteriophages were characterized to determine their role in the lysogenic conversion of bacteria and their possible usage in B. bronchiseptica control. For this purpose, morphological characteristics of the selected phages, the phage lytic spectrum, and the characteristics of phage genome and the proteins were examined. Also, the stability of phage in different environmental conditions was studied as well as their lytic efficiency, effect on the biofilm formation and formed biofilm. The paper also examined the role of phages in the production of biofilm, resistance to antibiotics, the process of hem olysis and the motility of B. bronchiseptica species. The results of this study clearly indicate the role of phage in lysogenic conversion in B. bronchiseptica as well as their potential for B. bronchiseptica control.