Ekstrakt talusa Usnea barbata (L.) Weber ex F. H. Wigg., Parameliaceae u emulzionim nosačima stabilizovanim biorazgradivim emulgatorima: preformulaciona i formulaciona istraživanja
Extract of the talus of Usnea barbata (L.) Weber ex F. H. Wigg., Parmeliaceae in the emulsion vehicles stabilized with biodegradable emulsifiers: preformulation and formulation investigations
Author
Žugić, Ana R.
Mentor
Savić, Snežana
Committee members
Vuleta, GordanaTadić, Vanja

Daniels, Rolf
Arsić, Ivana
Metadata
Show full item recordAbstract
Uvod Lokalna terapija antibiotskim preparatima predstavlja pogodan način prevencije i/ili
lečenja određenih infekcija kože, ali ima i niz ograničenja koja su vezana kako za lekovite
supstance sa antimikrobnim delovanjem, tako i za podloge koje se koriste kao njihovi
nosači. Pored porasta rezistencije bakterija, nedostatak dermalne primene antibiotika je i
njihova slaba penetracija u kožu, ali i toksičnost nekih od njih prema humanim ćelijama.
Usled toga, nameće se potreba za pronalaženjem novih supstanci sa antimikrobnom
aktivnošću širokog spektra dejstva i poboljšanom dermalnom
raspoloživošću/bezbednosnim profilom. Kao potencijalni izvori ovakvih supstanci sve više
se istražuju lišajevi, među kojima se naročito istakla Usnea barbata (L.) Weber ex F.H.
Wigg., Parmeliaceae (jevrejska ili lišaj brada). Iako se brojna biološka svojstva lišaj brade
pripisuju usninskoj kiselini, novija istraživanja ukazala su na neke prednosti upotrebe
njenog ekstrakta dobijenog primenom natkritičnog ugljen...-dioksida (CO2) u odnosu na ovu
supstancu per se. Sa druge strane, poznato je da su emulzioni sistemi (naročito kremovi),
kao najpopularniji nosači lekovitih supstanci u dermatološkim preparatima, često
stabilizovani tradicionalnim anjonskim/nejonskim mešanim emulgatorima, koji mogu
izazvati neželjene reakcije, posebno na oboleloj koži. Pored toga, značajan uticaj
kozmetičke industrije na dermofarmaciju je, uz porast zabrinutosti za zaštitu životne
sredine, stavio u fokus istraživanja savremenih emulgatora povoljnijeg ekološkog i
dermatološkog profila, koji istovremeno mogu da obezbede poboljšane senzorne osobine
kremova za primenu na koži.
Cilj istraživanja Cilj istraživanja ove doktorske disertacije bio je razvoj emulzionog
sistema tipa hidrofilnog krema sa biorazgradivim alkil poligukozidnim (APG)
emulgatorima prirodnog porekla, kao nosača za ekstrakt talusa U. barbata sa
kvantifikovanom usninskom kiselinom, kroz procenu koloidne strukture, fizičke stabilnosti
i biofarmaceutskih karakteristika, te ispitivanja efikasnosti i bezbednosti prototip
formulacije gotovog proizvoda, koji bi našao potencijalnu primenu kao alternativa
antibiotskim preparatima u lokalnoj terapiji infekcija kože...
Introduction Local therapy with antibiotic preparations represents the preferable way of
prevention and/or treatment of some skin infections but also has a number of limitations
connected to antimicrobial agents on one the hand and vehicles used as their carriers, on the
other. Aside from bacterial resistance growth, a major problem regarding dermal
application of antibiotics is their poor penetration into the skin, and also toxicity to human
cells, documented for some of them. Therefore, there is a growing interest in the research
concerning new substances with broad-spectrum antimicrobial activity and improved
dermal availability/safety profile. Lately, various lichens are being intensively investigated
as potential sources of such substances, among which Usnea barbata (L.) Weber ex F. H.
Wigg., Parmeliaceae (old man’s beard) has been singled out, as one of the most frequently
investigated. Although various biological properties of old man’s beard have been
attributed to usnic acid, ...recent research suggested certain benefits of usage of its extract
obtained using supercritical carbon dioxide (CO2), compared to this compound per se. On
the other hand, it is well established that the emulsion systems (particularly creams), as the
most popular carriers in dermatological preparations, are often stabilized using
conventional anionic/non-ionic emulsifiers, known to cause adverse effects, especially in
the diseased skin. Besides that, a major impact of the cosmetic industry on dermopharmacy,
alongside with the growing environmental concerns, has put in the focus research of
contemporary emulsifiers with favorable dermatological and ecological profile, which are
also able to provide enhanced sensory properties of creams for skin application.
Aim The aim of this doctoral dissertation was the development of an emulsion system of
hydrophilic cream type based on biodegradable alkyl polyglucoside (APG) emulsifiers, as a
potential carrier for the extract of the talus of U. barbata with quantified usnic acid,
throughout the assessment of colloidal structure, physical stability and biopharmaceutical
characteristics and also evaluation of efficacy and safety of the prototype formulation of the
final product, which could potentially serve as an alternative to the antibiotic preparations
in the local treatment of skin infections...
Faculty:
Универзитет у Београду, Фармацеутски факултетDate:
28-09-2016Projects:
- Functional physiologically active plant materials with additional values for application in pharmaceutical and food industry (RS-45017)
- Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia (RS-41030)