Uticaj infekcije virusom humane imunodeficijencije na genetičku varijabilnost kliničkih izolata BK i JC poliomavirusa
The influence of human immunodeficiency viral infection on the genetic variability of clinical isolates of BK and JC polyomaviruses
Author
Karalić, Danijela Z.Mentor
Lazarević, Ivana
Committee members
Jovanović, TanjaĆupić, Maja

Stanković-Đorđević, Dobrila
Metadata
Show full item recordAbstract
Uvod
Seroepidemiološke studije pokazuju da je između 27% i 80% humane
populacije inficirano sa dva poliomavirusa BK virusom (BKV) i JC virusom (JCV).
Posle primarne asimptomatske infekcije, koja se obično dešava u toku ranog
detinjstva, oba virusa uspostavljaju latenciju u ćelijama bubrega i gornjih partija
urinarnog trakta. Sporadična asimptomatska reaktivacija oba virusa se dešava kod
0.5-20% imunokompetentnih seropozitivnih osoba. Međutim, kod
imunosuprimiranih osoba njihova reaktivacija može dovesti do nastanka životno
ugrožavajućih oboljenja. Reaktivacija JCV, pre svega kod pacijenata sa T ćelijskom
imunodeficijencijom, dovodi do litičke infekcije oligodendrocita u centralnom
nervnom sistemu (CNS) i nastanka progresivne multifokalne leukoencefalopatije
(PML), dok reaktivacija BKV dovodi do nastanka hemoragijskog cistitisa i
poliomavirus-udružene nefropatije. Pre epidemije AIDS-a, limfoproliferativna
oboljenja su bila vodeći uzrok reaktivacije JCV i BKV, dok danas HIV infekcija
pre...dstavlja najčešći uzrok reaktivacije JCV i nastanka PML. Očigledno je da
imunosupresija predstavlja fundamentalni predisponirajući faktor za nastanak
PML, poliomavirus-udružene nefropatije i hemoragijskog cistitisa. Ipak, ova
oboljenja ne nastaju kod svih imunosuprimiranih osoba, tako da se smatra da i
drugi faktori, poput genetičke varijabilnosti BK i JC virusa, mogu doprineti
njihovom nastanku.
Ciljevi
Ciljevi ovog istraživanja bili su: da se odredi prevalenca izlučivanja BKV i JCV
u urinu HIV-inficiranih pacijenata i pacijenata kontrolne grupe i ispita moguća
povezanost virurije u grupi HIV-inficiranih sa demografskim, virusološkim i
citološkim parametrima kao i sa stadijumima bolesti i vrstom primenjene terapije;
da se utvrdi distribucija genotipova i subtipova, struktura nekodirajućeg
regulatornog regiona kao i prisustvo mutacija u VP1 regionu JCV i BKV u obe grupe
ispitanika...
Introduction
Seroepidemiologic studies have shown that between 27% and 80% of
human population is infected with two human polyomaviruses BK virus (BKV) and
JC virus (JCV). After primary asymptomatic infection, which usually occurs during
early childhood, both viruses establish latency in the cells of the kidney and upper
parts of the urinary tract. Sporadic asymptomatic reactivations of both viruses
occur in 0,5-20% of seropositive immunocompetent individuals. However, in
immunosuppressed patients reactivation of the viruses can lead to the
development of serious and life threatening diseases. Reactivation of JCV, primarily
in patients with T cell immunodeficiency, leads to lytic infection of
oligodendrocytes in the central nervous system (CNS) and the development of
progressive multifocal leukoencephalopathy (PML), while reactivation of BKV is
associated with hemorrhagic cystitis and polyomavirus-associated nephropathy.
Before the AIDS epidemic, lymphoproliferative diseases were the l...eading cause for
reactivation of JCV and BKV while nowdays HIV infection is the most common
cause for reactivation of JCV and the development of PML. It is evident that
immunosuppression is a fundamental predisposing factor for developing PML,
polyomavirus-associated nephropathy and hemorrhagic cystitis. However, these
diseases do not affect all immunosuppressed individuals, suggesting that other
factors, such as genetic variability of BK and JC viruses, can contribute to their
occurrence.
Objectives
The objectives of this research were to determine the prevalence of BKV and
JCV viruria in HIV-infected patients and patients of the control group and critically
evaluate the relationship between viruria and demographic, virological and
cytological parameters as well as the stages of the disease and the applied therapy;
also, to determine the distribution of genotypes and subtypes, the structure of noncoding
regulatory region and the presence of mutations in the VP1 region of JCV
and BKV in both groups of patients...