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Proizvodnja mikrobnih lipaza i proteaza kao aditiva u formulacijama detergenata

Production of microbial lipases and proteases as additives in detergent formulations.

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2014
Disertacija4348.pdf (3.880Mb)
Author
Grbavčić, Sanja Ž.
Mentor
Knežević-Jugović, Zorica
Committee members
Bezbradica, Dejan
Dimitrijević, Suzana
Karadžić, Ivanka
Metadata
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Abstract
U okviru ove teze, ispitan je veliki broj mikroorganizama sa aspekta produkcije ekstracelularnih lipaza i proteaza koje bi se potencijalno koristile kao aditivi u detergentima. Selekcija proizvodnih mikroorganizama obuhvatila je nekoliko faza kako bi se identifikovali mikroorganizmi koji proizvode lipaze i proteaze dobrih svojstava i u dobrom prinosu. Ove studije su izdvojile dva mikrobna producenta traženih enzima, koji pokazuju neophodnu funkcionalnost u alkalnim uslovima i u širokom spektru temperatura. Dobijene lipaze iz Pseudomonas putida B-21 i lipaze i proteaze iz Pseudomonas aeruginosa san-ai su zatim ispitane sa aspekta stabilnosti u prisustvu površinski aktivnih materija, oksidacionih agenasa i komercijalnih detergenata kako bi se utvrdila primenljivost dobijenih enzima kao aditiva u formulacijama detergenata. Uzevši u obzir pokazanu stabinost enzima koje produkuje P. aeruginosa san-ai oni su primenjeni u formulaciji nekoliko novih proizvoda koji pokazuju zadovoljavajuća svoj...stva sa stanovišta efikasnosti čišćenja, stabilnosti pri skladištenju i sastava otpadnih voda od pranja. Enzimska formulacija detergenata je optimizovana metodom odzivnih površina (RSM). Uzimajući u obzir ekološke aspekte kao i pokazanu efikasnost, pokazano je da se najbolji efekti odmašćivanja postižu u formulaciji koja pored enzima sadrži nisku dozu Lutensol®-a XP 80 (0,4%). Dalja optimizacija produkcije enzima RSM metodom definisala je sastave podloga i procesne parametre kojima se modifikuju regulatorni putevi biosinteze enzima sa ciljem povećanja prinosa lipaza i proteaza. Visoka proteolitička aktivnost se tako može ostvariti fermentacijom u medijumu koji je obogaćen Triton®-om X-100 (0.12% w/v) pri čemu proces fermentacije treba voditi na 32 °C. Niže temperature (20 °C) i suplementacija suncokretovim uljem pogoduju biosiontezi lipaze.

In this thesis, a vast number of microorganisms was tested in terms of the production of extracellular proteases and lipases with the ability to be used as detergent additives. These studies have marked two microbial strains which produce enzymes that show the necessary functionalities in alkaline conditions and a wide range of temperatures. A lipase derived from Pseudomonas putida B-21, and protease and a lipase from Pseudomonas aeruginosa san-ai were further tested with respect to stability in the presence of surfactants, oxidizing agents and commercial detergents in order to determine the applicability of the obtained enzymes in detergent formulations. Taking into the account demonstrated properties of lipase and protease produced by P. aeruginosa san-ai, these enzymes were applied in the formulation of several new products that demonstrate satisfactory performance in terms of cleaning efficiency, storage stability and wastewater composition. Enzyme detergent formulation was optimiz...ed using respond surface methodology (RSM). Considering the environmental aspects and demonstrated degreasing efficacy, detergent formulation which contains the produced enzymes should be based on low-dose Lutensol® XP 80 (0.4 %). Further optimization of the enzymes production using RSM defined medium composition and process parameters in order to increase the yield of lipases and proteases produced. The high proteolytic activity can be accomplished by fermentation in a medium enriched with Triton® X-100 (0.12% w/v) and the process should be run at 32 °C. Lower temperature (20 °C) and sunflower oil supplementation will favor lipase biosynthesis.

Faculty:
Универзитет у Београду, Технолошко-металуршки факултет
Date:
29-09-2014
Projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
Keywords:
lipaza / lipases / proteases / detergents / enzyme production / proteaza / detergenti / produkcija enzima
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_nardus_6370
URI
https://nardus.mpn.gov.rs/handle/123456789/6370
http://eteze.bg.ac.rs/application/showtheses?thesesId=3706
https://fedorabg.bg.ac.rs/fedora/get/o:12521/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47589135

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