Strukturne promene sluzokože debelog creva pacova pod uticajem akrilamida

Abstract

Akrilamid je supstanca koja se prirodno stvara pečenjem i prženjem hrane bogate skrobom. Cilj rada je bio da se ispita subhronični i akutni uticaj akrilamida na debelo crevo prepubertalnih pacova. Dobijeni rezultati su pokazali da akrilamid ne narušava morfologiju zida creva, ali dovodi do promena u volumenskoj gustini njegovih tunika i lamina. Najizraženije promene su bile na vezivnom tkivu debelog creva. Analizom peharastih ćelija i sadržaja mucina pokazano je da akrilamid utiče i na ugljenohidratnu i na proteinsku komponentu mucina. Subhronični tretman je doveo do smanjenja broja limfocita i eozinofila, a kod akutnog tretmana je primećeno nakupljanje limfocita i eozinofila u kolonu akrilamidom tretiranih jedinki. Broj mastocita je u sva tri eksperimenta bio smanjen kod tretiranih životinja. Duže izlaganje akrilamidu ima imunosupresivno dejstvo kod pacova.

Acrylamide is natural product of cooking (baking, roasting) starchy food. Aim of the study was to evaluate the risk of subchronic and acute acrylamide treatment on juvenile rat colon. Changes in colon wall morphology was detected by stereological methods since histological evaluation reveal normal colon architecture after acrylamide intoxication. The changes was most prominent on connective tissue of rat colon. Acrylamide affected both protein component of mucins and glycans linked to peptide backbone. In subchronic treatment acrylamide caused reduction of lymphocytes and eosinophils number, while acute experiment lead to lymphocytes and eosinophils accumulation in colon tissue. Acrylamide intoxication decreased mast cell number in all experiments. Longer acrylamide exposure had immunosuppressive effect in rats.