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Significance of disorder of the cell cycle regulator genes and expression of their proteins in rhabdomyosarcoma

dc.contributor.advisorSopta, Jelena
dc.contributor.otherVasiljević, Jovan
dc.contributor.otherBasta-Jovanović, Gordana
dc.contributor.otherBunjevački, Vera
dc.contributor.otherRadojević-Škodrić, Sanja
dc.creatorGlumac, Sofija Đ.
dc.date.accessioned2016-07-16T13:03:13Z
dc.date.available2016-07-16T13:03:13Z
dc.date.available2020-07-03T08:56:17Z
dc.date.issued2016-04-04
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=3250
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/5842
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:11643/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48017679
dc.description.abstractCilj: Rabdomiosarkom (RMS) je tumor visokog stepena maligniteta. Poslednje dve decenije prognoza pacijenata sa ovim tumorom je značajno poboljšana, osim kod pacijenata iz visokorizične grupe. U cilju utvrđivanja novih prognostičkih faktora ispitivali smo imunohistohemijsku ekspresiju proteina regulatora ćelijskog ciklusa i apoptoze, kao i obrasce metilacije promotora gena p16 u slučajevima RMS, i dobijene rezultate korelirati sa kliničkopatološkim parametrima. Metodologija: Ova studija je obuhvatila 67 slučajeva RMS dijagnostikovanih na Institutu za patologiju Medicinskog fakulteta, Univerziteta u Beogradu, u periodu od 1994-2014 godine Analizirani su sledeći kliničko-morfološki podaci: pol, starost, lokalizacija i veličina tumora, histološki tip i gradus, zahvaćenost regionalnih limfnih nodusa i postojanje metastatske bo lesti. Rezultati: Evaluacijom ekspresije ciklina A u odnosu na kliničko-patološke parametre pacijenata sa RMS, uočena je statistička značajnost samo u odnosu na histolški tip tumora (p=0.047). U ćelijama RMS, ciklin D1 je pokazivao jedarnu ekspresiju i aberantna ekspresija ovog markera nije pokazivala značajnu korelaciju sa ispitivanim parametrima. Rezultati različite ekspresija p57 u ovom tumoru su pokazivali statističku značajnost u odnosu na histološki tip (p=0,042),gradus tumora (p=0,026) i u odnosu na ishod bolesti (p=0,021). Ekspresija survivina u tumorskim ćelijama RMS bila je citoplazmatska i jedarna, Za razliku od citoplazmatske ekspresije survivina gde nije uočena njena povezanost sa prognostičkim faktorima, jedarna ekspresije ovog markera je bila statistički značajna u odnosu na starosne grupe, histološki tip i gradus tumora (p=0.009, p=0.008, p=0.010, redom). Analiza ekspresije nestina je pokazala statistički značaj u odnosu na starost pacijeanta (p = 0,028) i gradus tumora (p = 0,022)...sr
dc.description.abstractObjective: Rhabdomyosarcoma (RMS) is a tumor of high-grade malignancy. During the last two decades the prognosis of patients with this tumor has been significantly improved, except in patients from the high-risk group. In order to determine new prognostic factors, we investigated the immunohistochemical expression of proteins, the regulators of cell cycles and apoptosis as well as the patterns of methylation of the p16 gene promoter in cases of RMS, and the results obtained were to be correlated with clinicopathological parameters. Methodology: This study included 67 cases of RMS diagnosed at the Institute of Pathology, School of Medicine, University of Belgrade, in the period from 1994 to 2014. The following clinical and morphological data were analyzed: gender, age, tumor size and localization, histological type and grade, affected regional lymph nodes and the presence of metastatic disease. Results: By evaluation of the expression of cyclin A in relation to the clinicopathological parameters of patients with RMS, it was found a statistical significance only in relation to the histological type of tumor (p = 0.047). In the RMS cells, cyclin D1 showed a nuclear expression and the aberrant expression of this marker did not show any significant correlation with the studied parameters. Results of different expressions of p57 in this tumor showed a statistical significance in relation to the histological type (p = 0.042), tumor grade (p = 0.026) and in relation to the disease outcome (p = 0.021). The survivin expression in RMS tumor cells was cytoplasmic and nuclear. Unlike cytoplasmic survivin expression where no correlation with prognostic factors was found out, the nuclear expression of this marker was statistically significant in relation to the age groups, histological type of tumor and tumor grade (p = 0.009 , p = 0.008, p = 0.010, respectively).Analysis of the expression of nestin showed statistical significance in relation to the age of patients (p = 0.028) and tumor grade (p = 0.022)...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Медицински факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectRabdomiosarkomsr
dc.subjectRhabdomyosarcomaen
dc.subjectcell cycleen
dc.subjectapoptosisen
dc.subjectmethylationen
dc.subjectsurvivalen
dc.subjectćelijski ciklussr
dc.subjectapoptozasr
dc.subjectmetilacijasr
dc.subjectpreživljavanjesr
dc.titleZnačaj poremećaja gena regulatora ćelijskog ciklusa i ekspresije njihovih proteina u rabdomiosarkomusr
dc.titleSignificance of disorder of the cell cycle regulator genes and expression of their proteins in rhabdomyosarcomaen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dcterms.abstractСопта, Јелена; Буњевачки, Вера; Радојевић-Шкодрић, Сања; Васиљевић, Јован; Баста-Јовановић, Гордана; Глумац, Софија Ђ.; Значај поремећаја гена регулатора ћелијског циклуса и експресије њихових протеина у рабдомиосаркому; Значај поремећаја гена регулатора ћелијског циклуса и експресије њихових протеина у рабдомиосаркому;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/11819/Disertacija3805.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/11820/Glumac_Sofija_D.pdf


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