Show simple item record

The distribution of opiate alkaloids in brain

dc.contributor.advisorMimica-Dukić, Neda
dc.contributor.otherPopović, Mira
dc.contributor.otherTasić, Miloš
dc.contributor.otherIvetić, Vesna
dc.contributor.otherMimica-Dukić, Neda
dc.creatorĐurendić-Brenesel, Maja
dc.date.accessioned2016-06-19T15:52:03Z
dc.date.available2016-06-19T15:52:03Z
dc.date.available2020-07-03T13:45:38Z
dc.date.issued2008-03-01
dc.identifier.urihttp://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija139393751956136.pdf?controlNumber=(BISIS)71988&fileName=139393751956136.pdf&id=1468&source=NaRDuS&language=srsr
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/5494
dc.identifier.urihttp://www.cris.uns.ac.rs/record.jsf?recordId=71988&source=NaRDuS&language=srsr
dc.description.abstractU ovoj doktorskoj disertaciji je uspešno izvršeno izolovanje opijatnih alkaloida iz humanih bioloških uzoraka (moždanog tkiva, krvi, urina i žuči) kao i bioloških uzoraka  eksperimentalnih životinja (moždanog tkiva i krvi) primenom postupka čvrsto-tečne ekstrakcije (SPE-Solid Phase Extraction). Modifikovan je postupak za kvalitativnu i kvantitativnu GC-MS (Gas Chromatography-Mass Spectrometry) analizu bioloških  uzoraka. Utvrđena je distribucija opijatnih alkaloida: morfina, kodeina, acetilkodeina, 6-acetilmorfina i heroina u humanim biološkim uzorcima moždanog tkiva (moždanoj kori, moždanom stablu, amigdali i bazalnim jedrima), pri čemu je najveći sadržaj  opijata određen u moždanoj kori i bazalnim jedrima, podjednako kod muških i ženskih osoba. Utvrđena je distribucija opijatnih alkaloida: morfina, kodeina, acetilkodeina, 6-acetilmorfina i heroina u biološkim uzorcima moždanog tkiva (moždanoj kori, moždanom  stablu, amigdali i bazalnim jedrima) i krvi eksperimentalnih životinja (pacova), u  različitim vremenskim periodima (5, 15, 45 i 120 minuta) od tretiranja životinja heroinom. Najveći sadržaj opijata je određen u moždanoj kori i bazalnim jedrima, podjednako kod mužjaka i ženki pacova ali u različitim vremenskim periodima. U uzorcima krvi je najveći sadržaj opijata određen u istom vremenskom periodu kod životinja oba pola, pri čemu su kod mužjaka određene znatno veće vrednosti koncentracija, što ukazuje na bržudistribuciju opijata iz krvi u mozak kod ženki u  odnosu na mužjake pacova. Utvrđeno je da je distribucija opijata u humanom moždanom tkivu kod pripadnika suprotnih polova kao i moždanom tkivu mužjaka i ženki pacova (nakon 120 minuta od tretiranja heroinom), identična. Ispitivanjem uticaja opijata  na markere oksidativnog stresa u jetri eksperimentalnih životinja suprotnih polova, utvrđeno je smanjenje aktivnosti enzima: katalaze (CAT), glutation-peroksidaze (GSH- Px), peroksidaze (Px) i ksantin-oksidaze (XOD).sr
dc.description.abstractOpiate alkaloids were successfully isolated from human biological samples (brain tissue, blood, urine, and bile) as well as from biological samples of experimental animals (brain tissue and blood) by applying procedure of solid-phase extraction (SPE). A modified procedure was worked out for qualitative and quantitative analysis of biological samples by gas chromatography-mass spectrometry (GC-MS). The distribution of opiate alkaloids:morphine, codeine, acetylcodeine, 6-acetylmorphine, and heroine in human biological samples of brain tissue (cortex, brain stem, amigdala and basal nuclei) was established, showing the highest content of opiates   in the cortex and basal nuclei, equal with male and female persons. It was established how the opiatealkaloids: morphine codeine, acetylcodeine, 6-acetylmorphine  and heroine are distributed in biological samples of brain tissue (cortex, brain stem, amigdala and basal nuclei) and blood of experimental animals  (rats) in different time periods (5, 15, 45 and 120 min) after the animal treatment with heroine. The highest content of opiates was found in the cortex and basal nuclei, equal in the male and female rats, but in different time periods. In blood samples, the highest content of opiates was measured in the same period with animals of both sexes, the concentration in the males being significantly higher, indicating a faster passage of the opiates from blood to brain in the female compared to male rats. Identical distribution of opiates was found in human brain tissue of both male and female subjects as in rats of both sexes (120 min after treatment with heroine). Study of the effect of opiates on the markers of oxidative stress in the liver of tested animals of opposite sexes showed a lowered activity of the following enzymes:  catalase (CAT), glutathion-peroxidase (GSH-Px), peroxidase (Px) and xanthine-xidase  (XOD).en
dc.languagesr (latin script)
dc.publisherУниверзитет у Новом Саду, Природно-математички факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Новом Садуsr
dc.subjectOpijatni alkaloidisr
dc.subjectOpiate alkaloidsen
dc.subjectheroinsr
dc.subjecthumani mozaksr
dc.subjectmozak pacovasr
dc.subjectmoždana korasr
dc.subjectbazalna jedrasr
dc.subjectSPEsr
dc.subjectGC-MSsr
dc.subjectmarkeri oksidativnog stresasr
dc.subjectjetrasr
dc.subjectheroineen
dc.subjecthuman brainen
dc.subjectrat brainen
dc.subjectcortexen
dc.subjectbasal nucleien
dc.subjectSPEen
dc.subjectGC-MSen
dc.subjectmarkers of oxidative stressen
dc.subjectliveren
dc.titleDistribucija opijatnih alkaloida u mozgusr
dc.titleThe distribution of opiate alkaloids in brainen
dc.typedoctoralThesissr
dc.rights.licenseCC0
dcterms.abstractМимица-Дукић Неда; Тасић Милош; Мимица-Дукић Неда; Иветић Весна; Поповић Мира; Ђурендић-Бренесел Маја; Дистрибуција опијатних алкалоида у мозгу; Дистрибуција опијатних алкалоида у мозгу;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/38733/Disertacija2788.pdf


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record