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Preparation of marigold (Calendula officinalis L.) extract using carbon dioxide under pressure and its microencapsulation in the polymer–surfactant system

dc.contributor.advisorSovilj, Verica
dc.contributor.otherLepojević, Žika
dc.contributor.otherSovilj, Verica
dc.contributor.otherPrimorac, Marija
dc.creatorPetrović, Lidija
dc.date.accessioned2016-05-28T14:50:29Z
dc.date.available2016-05-28T14:50:29Z
dc.date.available2020-07-03T13:54:52Z
dc.date.issued2010-07-02
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/5433
dc.identifier.urihttp://www.cris.uns.ac.rs/DownloadFileServlet/Disertacijadisertacija.pdf?controlNumber=(BISIS)81972&fileName=disertacija.pdf&id=451&source=NaRDuS&language=srsr
dc.identifier.urihttp://www.cris.uns.ac.rs/record.jsf?recordId=81972&source=NaRDuS&language=srsr
dc.description.abstractSavremene svetske tendencije upućuju na sve širu primenu ekstrakata lekovitog i aromatičnog bilja, kako u prehrambenim proizvodima– funkcionalna hrana, tako i u proizvodima farmaceutske i kozmetičke industrije. Ekstrakti biljnog materijala, dobijeni primenom ugljendioksida pod pritiskom, sadrže termički nepromenjene aktivne komponente, te se poslednjih godina sve više primenjuju u farmaceutskoj i prehrambenoj industriji. Cilj ove doktorske disertacije je da se ispita mogućnost inkorporiranja ekstrakta nevena (Calendula officinalis L.), kao farmakološki aktivne materije, u mikrokapsule sa ciljem zaštite od spoljašnjih uticaja, produžetka njegovog delovanja i proširenja mogućnosti primene. Za dobijanje ekstrakata nevena primenjeni su postupci ekstrakcije ugljendioksidom u tečnom i superkritičnom stanju. Definisani su uslovi pri kojima je moguće dobiti ekstrakat sa visokim sadržajem etarskog ulja, nosiocem gastro-intestinalnog delovanja (200 bar, 40oC). Totalni ekstrakt dobijen pod ovim uslovima ekstrakcije je odabran za dobijanje mikrokapsula. Ispitivana je mogućnosti primene polimer–PAM interakcije nejonskih derivata celuloze- hidroksipropilmetil celuloze (HPMC) i anjonske PAM- natrijum dodecilsulfata (SDS), za formiranje omotača mikrokapsula. Primenom konduktometrijske i viskozimetrijske metode, određene su karakteristične koncentracije pri kojima HPMC–SDS interakcija započinje i završava se. Definisan je uticaj osobina molekula HPMC (molekulska masa, stepen supstitucije, vrsta supstituenta) i temperature na širinu intervala interakcije i objašnjeni mehanizmi njihovog povezivanja, sa osvrtom na strukturu i osobine formiranih HPMC/SDS komleksa. Reološkim ispitivanjima pri različitim uslovima definisane su promene u ponašanju sistema u zavisnosti od HPMC–SDS interakcije. Ispitivan je uticaj interakcije na osobine 20% emulzija suncokretovog ulja u vodi određivanjem njihovih reoloških osobina, veličina i raspodela veličina kapi i praćenjem stabilnosti. Utvrđeno je da se u oblasti najizraženije HPMC–SDS interakcije, odnosno kada se na granici faza ulje-voda nalazi umrežen HPMC/SDS kompleks, dobijaju emulzije najveće stabilnosti, sa njajmanjim srednjim prečnikom kapi. Sušenjem emulzija, primenom spray drying postupka, dobijene su mikrokapsule uljnog sadržaja, stabilizovane kompleksom HPMC/SDS. Najbolje karakteristike mikrokapsula (mehanička otpornost, morfološke karakteristike, sposobnost redispergovanja, veličina i raspodela veličina čestica i količina inkapsuliranog ulja), dobijene u oblasti najizraženije interakcije. Dodatak odabranog CO2 ekstrakta nevena u uljnu fazu emulzija ne menja značajno njihove osobine, kao ni osobine iz njih dobijenih mikrokapsula. Ispitivanja sprovedena u ovoj doktorskoj disertaciji pokazala su da se osobine kompleksa polimer/PAM mogu iskoristiti za mikrokapsulaciju ulja kao nosača farmakološki aktivnih materija.sr
dc.description.abstractContemporary global trends in food- functional food, pharmaceutical and cosmetic industry as well have been focused on a wider medical plants extracts application during the recent decade. Plant extracts obtained by means of carbon dioxide under high pressure contained all unchanged active compounds from plant, so that they have became more popular for application in food and pharmaceuticals recently. The aim of this thesis was to investigate the possibility to incorporate marigold extract (Calendula officinalis L.), as a pharmacologicaly active compound, into microcapsules in order to protect them from surrounding medium, improve their activity and enlarge application. Marigold extracts were obtained by means of carbon dioxide- CO2 under subcritical and supercritical conditions. Extraction conditions under which obtained extract has high content of essential oil, responsible for gastrointestinal activity, were determined (200bar and 40oC). Total extract obtained under such conditions, was chosen for microcapsule preparation. Application possibility of polymer–surfactant interaction between non-ionic cellulose derivativehydroxypropylmethyl cellulose (HPMC) and anionic surfactant- sodium dodecylsulfate (SDS) to microcapsule wall formation was investigated. Characteristic concentrations at which interaction starts and ends were determined by means of conductometric and viscometric measurements. The influence of HPMC molecular characteristics (molecular weight, degree of substitution and substituents kind) and temperature on interaction were determined and, considering the structure and characteristics of HPMC/SDS complexes, binding mechanism was explained. The changes in HPMC-SDS system caused by their interaction were defined by rheological investigations that took place under various conditions. The influence of interaction on the properties of 20% sunflower oil/water emulsion was investigated by rheology measurement, particle size and particle size distribution determination and stability testing. It was provided that emulsions prepared in the region of pronounced HPMC–SDS interaction, where HPMC/SDS complex is adsorbed at the o/w interface, have highest stability and smallest particle mean diameter. Microcapsules were obtained by spray drying of emulsions stabilized with HPMC/SDS complex. The best characteristics (mechanical resistance, morphological characteristics, redispersing ability, particle size and particle size distribution and amount of encapsulated oil) have microcapsules obtained in the region of most pronounced interaction. Addition of marigold CO2 extract in to the oil phase of emulsions has no significant influence neither on their, nor on corresponding microcapsules characteristics Investigations conducted in this thesis showed that characteristics of polymer/surfactant complexes can be used in microencapsulation of oil as carrier of pharmacologically active compoundsen
dc.formatapplication/pdf
dc.languagesr (latin script)
dc.publisherУниверзитет у Новом Саду, Технолошки факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Новом Садуsr
dc.subjectCO2 ekstrakcija nevenasr
dc.subjectCO2 extraction of Marigolden
dc.subjectinterakcije polimer–PAMsr
dc.subjectmikrokapsulacijasr
dc.subjectpolymer–surfactant interactionsen
dc.subjectmicroencapsulationen
dc.titleDobijanje ekstrakta nevena (Calendula officinalis L.) ugljen dioksidom pod pritiskom i njegovo mikrokapsuliranje u sistemu polimer-površinski aktivna materijasr
dc.titlePreparation of marigold (Calendula officinalis L.) extract using carbon dioxide under pressure and its microencapsulation in the polymer–surfactant systemen
dc.typedoctoralThesisen
dc.rights.licenseCC0
dcterms.abstractСовиљ, Верица; Приморац, Марија; Лепојевић, Жика; Совиљ, Верица; Петровић, Лидија; Добијање екстракта невена (Цалендула оффициналис Л.) угљен диоксидом под притиском и његово микрокапсулирање у систему полимер-површински активна материја; Добијање екстракта невена (Цалендула оффициналис Л.) угљен диоксидом под притиском и његово микрокапсулирање у систему полимер-површински активна материја;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/40436/Disertacija2627.pdf


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