Primena Higučijeve fraktalne dimenzije i metode nezavisnih komponenata u analizi spontane bioelektrične aktivnosti Br neurona vinogradskog puža pod delovanjem konstantnog magnetnog polja i uabaina
Application of Higuchi fractal dimension and indepenedent component method in analysis of garden snail Br neuron bursting activity modulated by static magnetic field and ouabain.
Author
Kesić, Srđan Ž.
Mentor
Spasić, Slađana
Committee members
Anđus, Pavle
Janać, Branka
Đorđević, Marko

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Show full item recordAbstract
Nelinearne i napredne statističke metode, pored linearnih metoda, zauzimaju sve
značajnije mesto u analizi fizioloških signala, posebno u svetlu nelinearnog i haotičnog
ponašanja bioloških sistema. Stoga je zajednička upotreba Higučijeve fraktalne dimenzije i
analize nezavisnih komponentata (ICA), značajan i nov pristup u analizi signala a posebno u
analizi aktivnosti jednog neurona. Najprepoznatljiviji tip spontane bioelektrične aktivnosti
neurona beskičmenjaka i kičmenjaka jeste pojava akcionih potencijala u paketićima.
U radu je po prvi put primenjen, jedinstven i inovativan pristup u razdvajanju
komponenata spontane bioelektrične aktivnosti Br neurona vinogradskog puža i to na akcione
potencijale (AP), intervale između akcionih potencijala (ISI) i tihe intervale bez aktivnosti (IBI)
uz pomoć Higučijeve fraktalne dimenzije i aproksimacije Gausovim funkcijama. Taj
metodološki pristup je omogućio praćenje uticaja konstantnog magnetnog polja i uabaina
inhibitora Na+/K+ pumpe... na promene u kompleksnosti spontane bioelektrične aktivnosti Br
neurona. Sa druge strane, po prvi put je testirana upotreba ICA metode u različitim
eksperimentalnim uslovima po AP, ISI i IBI komponentama spontane bioelektrične aktivnosti.
Na taj način, u ovom radu demonstrirana je snaga zajedničke upotrebe navedenih metoda
uz predlog da se proširi njihova upotreba za potrebe analize spontano aktivnih neurona različitih
vrsta u fiziološkim i patološkim stanjima.
Nonlinear and advanced statistical methods, in addition to linear methods, occupy a
prominent place in the analysis of physiological signals, especially in light of the non-linear and
chaotic behavior of biological systems. Therefore, the use of Higuchi fractal dimension and
independent component analysis (ICA), reperesents a new approach to signal analysis, especially
regarding the activities of one neuron. The most recognizable type of spontaneous bioelectric
activity in neurons of invertebrates as well as vertebrates is the appearance of bursting activity.
This study presents a unique and innovative approach to the separation of the components
of spontaneous bioelectric activity of the garden snail Br neuron - action potential (AP),
interspike interval (ISI) and interburst interval (IBI), by using Higuchi’s fractal dimension and
Gaussian fitting functions. This methodological approach allows monitoring of the effect of
static magnetic field and the inhibitor of the Na+/K+ ...pump, ouabain, on the changes in the
complexity of the spontaneous bioelectric activity of the Br neuron. On the other hand, for the
first time ICA method was tested in different experimental conditions on AP, ISI and IBI
components of spontaneous bioelectric activity.
This study demonstrates the power of the common use of the above mentioned methods
and proposes to extend their use for the purpose of analyzing spontaneously active neurons of
different species in physiological and patological conditions.