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Mutations in breast cancer susceptibility genes in BRCA1 and BRCA2 negative hereditary breast and ovarian cancer families in Serbia

dc.contributor.advisorStamenković-Radak, Marina
dc.contributor.otherBranković-Magić, Mirjana
dc.contributor.otherRadulović, Siniša
dc.creatorKrivokuća, Ana M.
dc.date.accessioned2016-05-07T10:00:11Z
dc.date.available2016-05-07T10:00:11Z
dc.date.available2020-07-03T08:12:53Z
dc.date.issued2014-04-30
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=2829
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/5275
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:11030/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024911538
dc.description.abstractUvod: Nasledni karcinom čini 5-10% od svih slučajeva karcinoma dojke i prvenstveno je povezan sa štetnim mutacijama u visoko penetrabilnim genima BRCA1 i BRCA2. I pored intenzivnog istraživanja ostatka nasledne komponente, treći visoko penetrabilni gen u karcinomu dojke (BRCA3) još uvek nije pronadjen. Pod okriljem poligenog modela otkrivene su tri grupe alela koji doprinose riziku za karcinom dojke: visoko penetrabilni, koji povećavaju rizik na 40-85% (BRCA1, BRCA2, TP53), srednje penetrabilni (povećavaju rizik na 20-40%) kao što su CHEK2, RAD51, RAD51C i XRCC1, i učestale genske varijante koje su nisko penetrabilne. Pored štetnih mutacija u ovim genima, odredjene polimorfne varijante mogu uticati na efikasnost popravke grešaka u DNK i na nastanak karcinoma. Cilj: Obzirom da prisustvo mutacija u BRCA1/2 genima ne može da opiše celu genetičku osnovu naslednog karcinoma dojke, cilj ovog rada je istraživanje prisustva populaciono specifičnih mutacija u drugim genima (TP53, CHEK2, NBS1, RAD51, RAD51C i XRCC1) kod BRCA1/2 negativnog, naslednog karcinoma dojke u Srbiji. Utvrdjivanje asocijacije polimorfnih varijanti u ovim genima sa rizikom za nastanak karcinoma dojke, kao i njihove korelacije sa parametrima tumora i parametrima domaćina tumora, predstavlja dodatni cilj ovog rada. Materijal i metode: 57 ispitanika poreklom iz visokorizičnih porodica kod kojih je sekvenciranje BRCA1/2 gena dalo negativan rezultat (nasledni uzorak) i 107 pacijentkinja sa karcinomom dojke koji nije odredjen u odnosu na porodičnu istoriju bolesti (sistematski uzorak), uključeno je u ovu studiju. Kontrolnu grupu je činilo 114 briseva grlića materice zdravih žena sa normalnim Papa nalazom i bez prethodne istorije pre-kancerskih i kancerskih lezija...sr
dc.description.abstractBackground: 5-10% of all breast cancer cases are hereditary, and are often caused by deleterious mutations in high penetrance BRCA1 and BRCA2 genes. Despite significant efforts in unraveling the rest of the familial component, BRCA3 could not be identified. Under the polygenic model of inheritance, three groups of genetic predisposition alleles have been identified so far: high-risk genes, conferring 40–85% lifetime risk (BRCA1, BRCA2, TP53), moderate-risk genes (20–40% lifetime risk) including CHEK2, RAD51, RAD51C and XRCC1, and low-risk common alleles. Besides the deleterious mutations in these genes, certain polymorphic variants have functional significance in DNA repair pathways, and may be responsible for cancer development. Aim: Considering the fact that mutations in BRCA1/2 can’t explain the whole range of hereditary predisposition to breast cancer, we aimed to investigate the presence and frequency of population-specific mutations in other susceptibility genes (TP53, CHEK2, NBS1, RAD51, RAD51C and XRCC1) in BRCA1/2 negative hereditary breast cancer cases in Serbia. Determining association between polymorphic variants in these genes with breast cancer risk, as well as their correlation with patient’s characteristics and histological and pathological characteristics of breast cancer, were also the aims of this study. Material and methods: 57 subjects from high-risk families negative for small alterations in BRCA1/2 genes (hereditary group) and 107 breast cancer patients non-selected according to family history (consecutive group) were included in the study. The control group was consisted of 114 cervical smears of gynecological healthy women with normal Papa test results and without previous history of pre-cancer and cancer lesions. Large genomic rearrangements in BRCA1/2 genes were investigated by MLPA technique, while genotyping for specific CHEK2 mutations was done by allele specific PCR...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Биолошки факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectnasledni karcinom dojke i jajnikasr
dc.subjecthereditary breast and ovarian canceren
dc.subjectpoligeni modelsr
dc.subjectSNPovisr
dc.subjectpolygenic modelen
dc.subjectsusceptibility genesen
dc.subjectSNPsen
dc.titleMutacije u genima koji doprinose riziku za oboljevanje od karcinoma dojke i/ili jajnika kod BRCA1 i BRCA2 negativnih visokorizičnih porodica u Srbijisr
dc.titleMutations in breast cancer susceptibility genes in BRCA1 and BRCA2 negative hereditary breast and ovarian cancer families in Serbiaen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dcterms.abstractСтаменковић-Радак, Марина; Бранковић-Магић, Мирјана; Радуловић, Синиша; Кривокућа, Aна М.; Мутације у генима који доприносе ризику за обољевање од карцинома дојке и/или јајника код БРЦA1 и БРЦA2 негативних високоризичних породица у Србији; Мутације у генима који доприносе ризику за обољевање од карцинома дојке и/или јајника код БРЦA1 и БРЦA2 негативних високоризичних породица у Србији;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/2947/Disertacija942.pdf


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