Fiziološki i biohemijski aspekti propagacije endemičnih vrsta Micromeria pulegium (Rochel) Benth. i Micromeria croatica (Pers.) Schott in vitro
Physiological and biochemical aspects of propagation in endemic species Micromeria pulegium (Rochel) Benth. and Micromeria croatica (Pers.) Schoot in vitro
Author
Tošić, Svetlana M.Mentor
Stojičić, DraganaCommittee members
Janošević, Dušica
Stankov Jovanović, Vesna

Zlatković, Bojan
Cvetić-Antić, Tijana

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aromatičnih biljaka koje se koriste u tradicionalnoj medicini, hortikulturi, hemijskoj,
prehrambenoj i kozmetičkoj industriji. Retke, endemične i/ili ugrožene vrste familije
Lamiaceae predstavljaju poseban izazov za proučavanje, prevashodno u cilju očuvanja
specijskog diverziteta i njihovih populacija u prirodi, ali i zato što su izvor važnih
bioaktivnih molekula raznovrsnog dejstva (antimikrobna, antvirusna, fungicidna,
antioksidantna, citotoksična, alelohemijska, insekticidna aktivnost, itd.) i potencijalno
široke primene.
Usled izrazite heterogenosti, predstavnici roda Micromeria su bili često predmet
taksonomskih diskusija. Pripadnike sekcije Pseudomelissa gde spada i М. pulegium,
nakon molekularno genetičkih analiza hloroplastne DNK, Brauchler (2005) premešta u
rod Clinopodium. Ipak, u ovoj tezi je primenjen tradicionalni taksonomski koncept,
predložen od strane Harley-a i sar. (2004), koji podržava homogenost roda Micromeria,
pri čemu je sect. Pseudomelissa njegova sastavna jedin...ica. U skladu sa primenjenim
sistemom dve istraživane vrste su klasifikovane u idividualne sekcije roda: Micromeria
sect. Micromeria (M. croatica), odnosno Micromeria sect. Pseudomelissa (M.
pulegium).
Micromeria pulegium predstavlja endemičnu vrstu Južnih Karpata u Rumuniji,
sa enklavom u istočnoj Srbiji, a Micromeria croatica balkansku endemičnu vrstu čiji se
areal vezuje pre svega za planinski venac Dinarida. Njihove prirodne populacije imaju
mali broj jedinki i nastanjuju staništa koja su pod negativnim uticajem antropogenih
faktora. Imajući u vidu značaj roda Micromeria, kao i činjenicu da su pomenute vrste
retke i ugrožene, javila se potreba da se one gaje zarad očuvanja i istraživanja bez
pritiska na prirodne populacije i bez remećenja prirodnog genofonda. Iz pomenutih
razloga obe vrste roda Micromeria uvedene su kulturu biljnih tkiva in vitro.
U ovoj disertaciji vršeno je ispitivanje efekata regulatora rastenja na
morfogenezu i produkciju etarskih ulja endemičnih vrsta M. pulegium i M. croatica in vitro...
Plants from Lamiaceae family are prominent representatives of the edible, medicinal
or aromatic herbs used in traditional medicine, horticulture, chemical, cosmetic and food
industry. The rare, endemic and/or threatened species from family Lamiaceae pose a special
challenge for studying, primarily in order to preserve species diversity and their populations
in the wild, but also as they are source material for important bioactive molecules with
diverse activity (antimicrobial, antiviral, fungicidal, antioxidant, cytotoxic, allelochemical,
insecticidal and other types of activity) and potential broad spectrum of use.
Due to the pronounced heterogeneity, representatives of genus Micromeria used to be
a common topic of taxonomic discussions. After the molecular-genetic analyses of
chloroplast DNA, Brauchler (2005) has transferred the representatives of section
Pseudomelissa, which includes М. pulegium, into the genus Clinopodium. However, this
thesis is using the traditional taxonomic con...cept suggested by Harley et al. (2004),
supporting the homogeneity of genus Micromeria, where sect. Pseudomelissa remains its
integral unit. According to this system the two studied species were classified within the
individual sections of the genus: Micromeria sect. Micromeria (M. croatica) and Micromeria
sect. Pseudomelissa (M. pulegium).
Micromeria pulegium represents an endemic species of Southern Carpathians in
Romania, with an enclave in Eastern Serbia, while Micromeria croatica is an endemic
Balkan species primarily connected with the mountain chain of Dinarides. Their natural
populations are characterized by a small number of individuals inhabiting habitats under the
negative influence of anthropogenic factors. Due to the importance of genus Micromeria and
the fact that these two species are rare and threatened, a need arose to grow them in captivity
so they may be preserved and studied without additional pressure on natural populations and
without disturbance of the natural gene pool. For these reasons both species of genus
Micromeria were introduced in plant tissue culture in vitro...