Uloga i značaj alfa-lipoinske kiseline i nesteroidnoh antiinflamatornih lekova u hemoprevenciji i indukciji apoptoze ćelija karcinoma kolona i cerviksa - in vitro studija
Докторанд
Damnjanović, Ivana D.Ментор
Pešić, SrđanЧланови комисије
Najman, StevoKocić, Gordana
Stojanović, Dušica
Milovanović, Dragan
Метаподаци
Приказ свих података о дисертацијиСажетак
The colon and cervix cancer treatment is usually based on the activity of one or more
chemotherapeutic agents, which stop the cell cycle in numerous ways, by showing a high
level of adverse effects and the development of resistance. Therefore, finding new or
improving the existing agents with potential antitumor activity is imperative in the
contemporary pharmacotherapy of cancer. Increasing the efficiency of chemotherapeutic
agents can be achieved by combining the treatment with chemopreventive agents, which
would enhance the effectiveness of the cytostatics and reduce the side effects.
The aim of this study is to investigate the effect of the alpha-lipoic acid, ketoprofen,
meloxicam and the combinations of tese test substances with cisplatin and 5-fluorouracil, in
the cell cultures of colon and cervical cancer and the primary cultures of the mononuclear
cells of peripheral blood, on cell proliferation and the quantitative expression of NF-B, Bcl-
2 and Bax protein in order to assess... the test substances’ chemopreventive potential.
The MTT test in the cell proliferation assay was used to examine the effect of test
substances on the proliferation of colon and cervix cancer cells, while the quantitative
expression of the NF-B, Bcl-2 and Bax protein was determined in correspondence to the
immunofluorescence method. The role of the mononuclear cells of peripheral blood in the
conducted research, is based on monitoring the selectivity of the tested substances by
analysing the expression of mitochondrial apoptosis markers.
The research results show that the alpha-lipoic acid, ketoprofen and meloxicam have
an important role in the chemoprevention of colon and cervix cancer, by having an influence
on the expression of NF-B, Bcl-2 and Bax, as important markers of the apoptotic cell power
of the colon and cervix cancer cells. The test substances showed a dose-dependent effect,
especially in combination with the cytostatics. When it comes to the comparison of the
antiiflamatory nonsteroidal drugs, the priority could be given to meloxicam, probably due to
the more selective profile of the COX-2 inhibition. The results could be very good candidates
for the further in vivo preclinical studies in order to potentiate the effects, in a particular
reference to the development of resistance and the reduction of the side effects that occur in
the standard cytostatic treatment of colon and cervix cancer
Факултет:
Универзитет у Нишу, Медицински факултетДатум одбране:
23-01-2015Пројекти:
- Мониторинг електромагнетних зрачења мобилних телекомуникационих система у животној средини, анализа молекуларних механизама и биомаркера оштећења код хроничне изложености са развојем модела за процену ризика и метода за заштиту (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43012)