Transport LDL-a u u izolovanoj karotidi kunića pre i tokom razvoja ateroskleroz
LDL transport in isolated rabbit carotid before and during atherosclerosis
Committee membersPantović, Suzana
MetadataShow full item record
The study included 20 New Zealand White rabbits with surgically isolated segments of left and right common carotid artery. The animals were divided into four groups: control group- normal feeding and three experimental groups with 1% cholesterol added to normal feeding (atherogenic diet) during 8, 10 and 12 weeks. The isolated segment of rabbit carotid artery was used under constant perfusion flow and pressure-induced (0 mmHg and 140 mmHg) blood vessel distension, with the possibility to change and precisely calculate shear stress during the experiment. In order to examine 125I-LDL ransport the continual 10-min perfusion was used (100 μl/min) (Steady state method). The measured 125I-LDL specific activity in each sample was used to obtain radioactivity-time curves, that is, dilution profiles for 125I-LDL during its flow through the blood vessel at a set value of perfusion pressure. The evaluation of 125I LDL transport into isolated blood vessel wall was calculated as difference between... inflow and outflow dose of 125I-LDL-within the period of Steady state. Obtained results are showed and well documented with 3 tables, 8 figures and 2 mathematical equations. The results showed increased LDL transport after removal of endothelium and the pressure-induced (0 mmHg and 140 mmHg) blood vessel distension has its influence at LDL transport. It was showed that in conditions of prolonged hyperlipidaemia (atherogenic diet) with low pressure-induced blood vessel distension (0 mmHg) LDL transport into blood vessel wall is ,decreased as well as values of shear stress. However, with high pressure-induced blood vessel distension (140 mmHg) LDL transport into blood vessel wall is increased although values of shear stress increase too.
Faculty:University of Kragujevac, Faculty of Medical Sciences
- Multi-level patient-specific artery and atherogenesis modelfor outcome prediction, decision support treatment, andvirtual hand-on training (EU-224297)
- Application of biomedical engineering for preclinical and clinical practice (RS-41007)