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Development, preparation and characterization of solid selfdispersing formulations for oral application

dc.contributor.advisorIbrić, Svetlana
dc.contributor.otherĐurić, Zorica
dc.contributor.otherĐekić, Ljiljana
dc.contributor.otherVasiljević, Dragana
dc.contributor.otherGrujić, Branka
dc.creatorMilović, Mladen R.
dc.date.accessioned2020-07-03T09:47:14Z
dc.date.available2020-07-03T09:47:14Z
dc.date.issued2015-02-27
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/4650
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=2454
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:10467/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47557903
dc.description.abstractU radu su formulisane i ispitane i formulisane različiti čvrsti samodispergujući sistemi kao potencijalni nosači za teško rastvorljivu model lekovitu supstancu karbamazepin (CBZ). Ispitivani su: čvrsti samomikroemulgujući sistem (eng. Solid Self-microemulsifying drug delivery system, skr. SSMEDDS), čvrsta samomulgujuća fosfolipidna suspenzija (eng. Solid Self-emulsifying phospholipid suspension, skr. SSEPS) kao i samoemulgujuće pelete. Samomikroemulgujući sistem (eng. Self-microemulsifying drug delivery system - SMEDDS) je fomulisan korišćenjem surfaktanta (Polisorbat 80 (S)), kosurfaktanta (PEG – 40 hidrogenizovano ricinusovo ulje (C)) i uljane faze (trigliceridi srednje dužine lanca (O)). Prilikom izrade SSMEDDS sistema korišćena su četiri različita adsorbensa velike specifične aktivne površine, pri čemu su dva bila tipa magnezijum-aluminometasilikata (Neusilin® UFL2 i Neusilin® FL2) i dva tipa silicijum-dioksida (Sylysia® 320 i Sylysia® 350). Ispitana je mikroemulziona oblast sistema pri odnosu surfaktanta i kosurfaktanta (Km) 1:1 i SMEDDS formulacija sa surfaktant-kosurfaktant/ulje odnosom (eng. surfactant-cosurfactant/oil ratio - SC/O) 8/2 je izabrana za dalja istraživanja. Kapacitet solubilizacije odabrane SMEDDS formulacije za CBZ iznosio je 33771 ± 41 μg/ml. Reološka merenja SMEDDS formulacije sa i bez CBZ, pri sadržaju vode od 10 do 60 % (m/m), su pokazala da nakon razblaženja sa vodom CBZ ima znatan uticaj na reološko ponašanje ispitivanih sistema. Fotonska korelaciona spektroskopija je pokazala sposobnost formulacije da, nakon razblaženja sa vodom, formira kapi čiji prečnik odgovara mikroemulzijama. SSMEDDS formulacije su znatno poboljšale brzinu rastvaranja CBZ, pri čemu je pokazano da vrsta adsorbensa znatno utiče na brzinu rastvaranja. Kod formulacija sa adsorbensom tipa magnezijum-aluminometasilikata brzina rastvaranja CBZ opada sa porastom specifične aktivne površine, usled zadržavanja dela tečne SMEDDS unutar pora sistema i njegovom postepenom izlaganju spoljašnjem medijumu. SSMEDDS formulacije sa adorbensima na bazi silicijum-dioksida nisu pokazale značajnu razliku u odnosu na odgovarajuće fizičke smeše. Prisustvo amorfnog oblika CBZ u fizičkim smešama je razlog primećene velike brzine rastvaranja. Takođe je formulisana samoemulgujuća fosfolipidna suspenzija (eng. Self-emulsifying phospholipid suspension, skr. SEPS) korišćenjem PEG-8 kaprilno/kaprinski gliceridi (S)/(lecitin/propilen-glikol) (C)/trigliceridi srednje dužine lanca (O) samoemulgujućeg sistema kao disperznog medijuma. Km odnos 3:2 je ispitan i SC/O odnos 8:2 je uzet za dalja istraživanja. Fotonska korelaciona spektroskopija je pokazala sposobnost supernatanta SEPS da nakon razblaženja sa vodom formira kapi prečnika koji odgovara emulzijama. Dijatomitne mikrokapsule, koje predstavljaju fosilizovane skelete fotosintetičlih algi za kompleksnom trodimenzionalnom (3D) strukturom, porozne strukture, koje se sastoje od amorfnog silika materijala i koje se dobijaju prečišćavanjem dijatomejske zemlje, su korišćene kao adsorbensi za SEPS prilikom formulisanja SSEPS. Različiti čvrsti uzorci SSEPS su pripremljeni korišćenjem dve metode: adsorpcija disperzije CBZ u SEDDS na dijatomitne mikrokapsule blagim mešanjem u tarioniku sa pistilom (Metoda A); disperzijom dijatomitnih mikrokapsula u etanolnom rastvoru CBZ i SEDDS, nakon čega je etanol uparen (Metoda B). Brzina rastvaranja CBZ iz izrađenih SSEPS formulacija je bila znatno veća u poređenju sa čistim CBZ, ali i fizičkom smešom i odgovarajućom čvrstom disperzijom dobijenom uparavanjem etanolnog rastvora CBZ iz disperzije dijatomitnih mikrokapsula. Brzina rastvaranja CBZ iz SSEPS formulacije pripremljene metodom B je veća u poređenju sa brzinom rastvaranja CBZ iz SSEPS pripremljene metodom A. Razlog za ovo je verovatno parcijalna adsorpcija SSEPS formulacije pripremljene metodom B unutar pora dijatomitnih mikrokapsula...sr
dc.description.abstractThe purpose of this study was to investigate different solid self-dispersing formulations as potential drug delivery system for poorly soluble carbamazepine (CBZ). Solid selfmicroemulsifying drug delivery system (SSMEDDS), solid self-emulsifying phospholipid suspension (SSEPS) and self-emulsifying pellet formulations were investigated. Selfmicroemulsifying drug delivery system (SMEDDS) was formulated using the surfactant polyoxyethylene 20 sorbitan monooleate (S), the cosurfactant PEG-40 hydrogenated castor oil (C) and the oil caprylic/capric triglycerides (O). Four different adsorbents with high specific surface area were used: Neusilin® UFL2, Neusilin® FL2 (magnesium aluminometasilicate), Sylysia® 320 and Sylysia® 350 (porous silica). Microemulsion area at the surfactant to cosurfactant ratio (Km) 1:1 was evaluated and for further investigation SMEDDS with SC/O ratio 8:2 was selected. Solubilization capacity of selected SMEDDS for CBZ was 33771 ± 41 μg/ml. Rheological measurements of unloaded and CBZ-loaded SMEDDS system at water content varied from 10 to 60 % (w/w) were conducted. It has been found that CBZ has great influence on rheological behaviour of investigated system upon water dilution. Photon correlation spectroscopy has shown ability of CBZ-loaded SMEDDS system to produce microemulsion droplet size. SSMEDDS improved release rate of CBZ, but type of adsorbent significantly affects release rate of CBZ. For SSMEDDS with different magnesium aluminometasilicate adsorbents, release rate of CBZ decreased with increasing specific surface area due to entrapment of liquid SMEDDS inside the pores and its gradual exposure to dissolution medium. With porous silica adsorbents no difference in release rate was found in comparison to physical mixtures. In physical mixtures presence of amorphous CBZ led to high dissolution rate. Self – emulsifying phospholipid suspension (SEPS) was formulated using caprylocaproyl macrogol-8 glycerides (S)/ (lecithin/ propylene glycol) (Cs)/ caprylic/capric triglyceride (O) self-emulsifying system. Km 3:2 was evaluated and for further investigation SEDDS with SCs/O ratio 8:2 was selected. Photon correlation spectroscopy has shown ability of SEPS supernatant to produce emulsion droplet size Diatoms, which represent fossilized skeletons of photosynthetic algae with complex 3 – dimensional (3D), porous structure consisting of amorphous silica, obtained by purification of diatomaceous earth, was used as solid carrier. Different solid samples of CBZ suspension in SEDDS, called solid self – emulsifying phospholipid suspension (SSEPS), were prepared using two methods: adsorption of CBZ dispersion in SEDDS by gentle mixing with diatoms in mortar with pestle (Method A) or dispersion of diatoms in ethanol solution of CBZ and SEDDS components, followed by ethanol evaporation (Method B). Release rate of CBZ from SSEPS was significantly higher in comparison to pure drug, physical mixture of diatoms and CBZ as well as solid dispersion of pure CBZ and diatoms obtained by ethanol evaporation. The dissolution of CBZ from SSEPS sample prepared using method B was faster than from the sample prepared by the method A. Higher dissolution for sample prepared by the method B can be attributed to the partial adsorption (deeper localization) of liquid material inside the pores of diatoms. Upon storage of the samples under accelerated conditions (40°C and 70% RH) for 10 weeks no significant changes in CBZ crystallinity and dissolution was in case of SSEPS, contrary to solid dispersion with increased crystallinity, indicating that diatoms with adsorbed liquid CBZ – loaded SEPS can maintain initial CBZ characteristics. Self-emulsifying pellets were formulated by layering SEPS formulation on the surface of sugar pellets, 710- 825 μm i size, using fluid-bed coater and ethanol/water 9/1 dispersion medium. Optimized amounts of colloidal silica (anti-tacky agent) as well as hidroxypropilmethylcellulose (as binder) were used...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Фармацеутски факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectčvrsti samomikroemulgujući sistemsr
dc.subjectSolid self- microemulsifying drug delivery systemsen
dc.subjectčvrsta samoemulgujuća fosfolipidna suspenzijasr
dc.subjectsamoemulgujuće peletesr
dc.subjectmagnezijum-aluminometasilikatsr
dc.subjectporozni silicijum-dioksidsr
dc.subjectdijatomitne mikrokapsulesr
dc.subjectpovećanje brzine rastvaranjasr
dc.subjectdinamička in vitro lipolizasr
dc.subjectkarbamazepinsr
dc.subjectsolid self - emulsifying phospholipid suspensionen
dc.subjectself-emulsifying pelleten
dc.subjectmagnesium aluminometasilicateen
dc.subjectporous silicaen
dc.subjectdiatomen
dc.subjectdissolution rate improvementen
dc.subjectdynamic in vitro lipolysisen
dc.subjectlecithin carbamazepineen
dc.titleRazvoj, izrada i karakterizacija čvrstih samodispergujućih formulacija za oralnu primenusr
dc.titleDevelopment, preparation and characterization of solid selfdispersing formulations for oral applicationen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dcterms.abstractИбрић, Светлана; Ђурић, Зорица; Ђекић, Љиљана; Васиљевић, Драгана; Грујић, Бранка; Миловић, Младен Р.; Развој, израда и карактеризација чврстих самодиспергујућих формулација за оралну примену; Развој, израда и карактеризација чврстих самодиспергујућих формулација за оралну примену;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/24107/Mladen_Milovic_referat_FF.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/24106/Disertacija254.pdf


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