Ispitivanje uticaja aklimatizacije na promenu koncentracije stresnih hormona i činilaca hemostaze u serumu vojnika izloženih toplotnom stresu u fizičkom naporu
Investigation of the effect of acclimation on the concentration of stress hormones and hemostatic factors in serum of soldiers exposed to heat stress in physical activity
Author
Vesić, ZoranMentor
Jakovljević, Vladimir
Committee members
Rosić, Gvozden
Radaković, Sonja
Grujić, Nikola
Metadata
Show full item recordAbstract
Cilj ove studije je bio da ispita
toleranciju na toplotni stres usled
fizičkog napora u grupama ispitanika
podvrgnutih fizičkom naporu koji su
prethodno izloženi pasivnoj, odnosno
aktivnoj aklimatizaciji, kao i da
utvrdi uticaj aklimatizacije na
fizičku sposobnost i funkcije
hormonskog, enzimskog i hemostatskog
sistema.
Metode. Ispitivanjem je obuhvaćeno
ukupno 40 muškaraca, studenata iste
klase Vojne Akademije u Beogradu, odsek
KOV, starosti od 19-21 godine,
podeljenih metodom slučajnog izbora
prema tablicama slučajnih brojeva u 4
grupe (K, 0, P i A grupa). Ispitanici iz
K grupe su podvrgnuti testu
termotolerancije u komfornim
uslovima, uz submaksimalno fizičko
opterećenje; iz 0 grupe podvrgnuti su
istom testu u uslovima tople sredine,
P grupa se prethodno izlaže pasivnoj
10-dnevnoj aklimatizaciji, a A grupa
aktivnoj aklimatizaciji istog trajanja.
Ispitivanje antropometrijskih i
ergometrijskih karakteristika
obuhvatilo je: merenje telesne visine
(cm), teles...ne mase (kg), uhranjenosti i
sadržaja telesne masti. Ergometrijske
karakteristike (VO2max) ispitivane su
indirektno, preko pulsa, metodom po
Brusu, uz korišćenje pokretne trake.
Svakom ispitaniku je u dva navrata (pre
i posle testa toplotnog stresa) uzet
uzorak venske krvi. Biohemijski
parametri (broj leukocita i
trombocita, protrombinsko vreme,
antitrombin III, glukoza, amonijak i
BUN) mereni su standardnim metodama,
kao i sledeći enzimi (AST, ALT, LDH,
kreatin-kinaza) i hormoni: FT4, TSH,
kortizol, prolaktin, testosteron i
insulin.
Rezultati. Rezultati istraživanja
sistematično su prikazani i dobro
dokumentovani sa 23 tabele i 30
grafikona. Pokazano je da: 1) postoji
visok stepen homogenosti studijskog
uzorka, 2) između ispitivanih grupa
nije bilo razlike u vrednostima
VO2max, 3) aklimatizacija poboljšava
sposobnost organizma da održi
termoregulaciju tokom fizičke
aktivnosti uz prisustvo toplotnog
stresa, 4) ispitanici podvrgnuti
aklimatizaciji su imali niže
vrednosti srčane frekvence dok vrsta
aklimatizacije ne menja značajnije njen
efekat na brzinu rada srca; 5) analiza
biohemijskih parametara generalno
pokazuje da aklimatizacija povoljno
deluje na ublažavanje akutnih upalnih
procesa i time ograničava posledična
mišićna oštećenja, pri čemu izgleda
da pasivna aklimatizacija ostvaruje
pozitivnije dejstvo, 6) aklimatizacija
može biti od značaja za kontrolu
koagulacionog statusa tokom toplotnog
stresa, 7) aklimatizacija nije imala
bitniji uticaj na funkciju
ispitivanih endokrinih žlezda tokom
toplotnog stresa (osim prolaktina).
Zaključak. Aklimatizacija poboljšava
sposobnost organizma da održi
termoregulaciju tokom fizičke
aktivnosti uz prisustvo toplotnog
stresa, dok oblik aklimatizacije
(pasivna ili aktivna) ne menja bitno
efekat izlaganja samoj aklimatizaciji.
Analizom biohemijskih parametra
generalno možemo zaključiti da
aklimatizacija povoljno deluje na
ublažavanje akutnih upalnih procesa i
time ograničava posledična mišićna
oštećenja, pri čemu izgleda da pasivna
aklimatizacija ostvaruje pozitivnije
dejstvo. Generalno posmatrano pasivni
tip aklimatizacije, može biti od
značaja za kontrolu pro-/anti
koagulacionog statusa koji time može
zadobiti veću pažnju u smislu
uključenja ove procedure u pripremu
vojnika tokom boravka u toplim
klimatskim uslovima. Sumarno
posmatrano, uticaj aklimatizacije na
hormonske pokazatelje ističu njen
značaj u odgovoru endokrinog sistema
vojnika za obavljanje vojnih aktivnosti
u toplim klimatskim uslovima.
he aim of this study was to
investigate the tolerance to heat stress
due to physical exertion in groups of
subjects undergoing physical exertion
that were previously exposed to passive,
or active acclimatization, as well as to
determine the effect of acclimatization on
physical ability and function of the
hormone, enzymatic, and the hemostatic
system.
Methods. The study involved 40 men,
students of the same class of the Military
Academy in Belgrade, Department of
KOV, aged 19-21 years, divided
randomly according to the table of
random numbers into 4 groups (K, 0, P
and A group). Respondents from K group
were subjected to test thermotolerance in
comfortable conditions, with submaximal
physical load; from 0 group subjected to
same test conditions in a warm
environment; P group previously exposed
to passive 10-day acclimatization; and A
group of active acclimatization of the
same duration. Investigation of
anthropometric and ergometric
characteristics included: b...ody height
(cm), body weight (kg), nutritional status
and body fat composition. Ergometer
characteristics (VO2max) were studied
indirectly through pulse method by
Bruce, with the use of a conveyor belt.
Each subject, uderwent venous blood
sampling, on two occasions (before and
after heat stress test). Biochemical
parameters (white blood cell and platelets
count, prothrombin time, antithrombin
III, glucose , ammonia, and BUN) were
measured by standard methods, and the
following enzymes (AST, ALT , LDH,
creatine kinase), and hormones: FT4,
TSH, cortisol, prolactin, testosterone and
insulin.
Results. The results are systematically
presented and well documented within 23
tables and 30 charts. It is shown that: 1)
there is a high degree of homogeneity of
the study sample, 2) between the groups
there were no difference in the values of
VO2max, 3) acclimation improves the
body's ability to maintain
thermoregulation during exercise in the
presence of heat stress, 4) subjects
underwent acclimatization had lower
heart rate, while type of acclimation does
not change significantly heart rate; 5)
analysis of biochemical parameters in
generally shows that beneficial effect of
acclimatization on the alleviation of acute
inflammatory processes, and thus
limiting consequential damage of
muscles, where it appears that the passive
acclimation achieves more positive
effects, 6) acclimatization may be
important in control of coagulation
during the heat stress, 7) acclimation
does not have significant influence on the
function of an endocrine glands (except
prolactin) during heat stress.
Conclusion. Acclimatization improves
body's ability to maintain
thermoregulation during exercise in the
presence of heat stress, while the type of
acclimatization (passive or active) does
not change significantly the effect of
acclimatization itself. By the analysis of
biochemical parameters, in general, it can
be concluded beneficial effect of
acclimatization on the alleviation of acute
inflammatory processes, and thus
limiting consequential muscle damage,
where it appears that passive
acclimatization achieved more positive
influence. Generally viewed, passive
acclimatization may be of importance for
the control of pro-/anti coagulation time,
which can paid more attention in terms of
involvement of these procedures in the
preparation of soldiers during warm
enviorement. In summary, the effect of
acclimatization on hormonal indicators
highlight its importance in the response
of the endocrine system of soldiers
performing military activities in warm climates.