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Biohemijske karakteristike izabranih vrsta gljiva u funkciji biodegradacije deterdženata

Biochemical characteristics of selected species of fungi in the function of biodegradation detergent

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2014
Disertacija.pdf (7.771Mb)
Author
Jakovljević, Violeta D.
Mentor
Stojanović, Jelica
Committee members
Vrvić, Miroslav M.
Manojlović, Nedeljko
Metadata
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Abstract
Cilj ovog istraživanja bio je ispitivanje uticaja visokih koncentracija komercijalnog deterdženta „Merix“, (Henkel, Kruševac) na rast, razvoj i biohemijske karakteristike testiranih gljiva koje su izolovane iz kanalizacionih i industrijskih otpadnih voda. Gljive su gajene u tečnoj hranljivoj podlozi po Čapeku (kontrola) i istoj podlozi sa dodatkom deterdženta u koncentraciji 0,3% i 0,5%. U oglednom periodu od 3. do 16. dana ispitivane su promene: pH i redoks potencijala; količine proteina, slobodnih i ukupnih organskih kiselina; kvantitativni I kvalitativni sastav aminokiselina, kao i aktivnost određenih enzima u fermentacionoj tečnosti. Istovremeno je ispitivana i koncentracija deterdženta (anjonskih komponenti deterdženta) u fermentacionoj tečnosti pomoću MBAS metode. Sve gljive su razgradile deterdžent 0,3% koncentracije, a M. racemosus je razgradila i deterdžent 0,5% koncentracije. Deterdžent je uticao na inhibiciju biomase gljiva u različitom procentu u zavisnosti od vrste gljiv...a i mesta izolovanja. Kod gljive M. racemosus deterdžent je delovao stimulativno na biomasu, tako da je veća biomasa izmerena u podlozi sa 0,5% deterdžentom. Prisustvo deterdženta u hranljivoj podlozi i njegovih produkata degradacije tokom fermentacije gljiva uticalo je na promene svih ispitivanih biohemijskih parametara i enzimske aktivnosti.

The aim of this study was to investigate the effect of high concentrations of commercial detergent "Merix" (Henkel, Krusevac) on growth and development and biochemical characteristics of the tested fungi were isolated from sewage and industrial wastewater. Fungi were grown in a liquid Czapek nutrient medium (control) and in the same medium with the addition of 0.3% and 0.5% detergent. The changing of pH values and redox potential; amounts of protein, free and total organic acids, the qualitative and quantitative composition of the amino acids, as well as the activity of certain enzymes were investigated in the fermentation liquid during the experimental period from 3 to 16 days. At the same time, concentration of detergent (anionic components of the detergent) in the fermentation broth was examined using MBAS method. All of the tested fungi were degrade detergent in concentration of 0.3%, but only M. racemosus decompose detergent in concentration of 0.5%. Detergent influenced t...he inhibition of the fungal biomass in varying percentages depending on the type of fungus and isolation. Detergent has a stimulating effect on biomass of fungus M. racemosus, so a greater biomass is measured in a medium containing 0.5% detergent. The presence of detergent in the nutrient medium and its degradation products during fermentation of fungi influenced the change of all the examined biochemical parameters and enzyme activities.

Faculty:
Универзитет у Крагујевцу, Природно-математички факултет
Date:
30-05-2014
Projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
Keywords:
aminokiseline / biomasa / enzimska aktivnost organske kiseline / proteini / pH vrednost / redoks potencijal.
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_nardus_3605
URI
https://nardus.mpn.gov.rs/handle/123456789/3605
http://eteze.kg.ac.rs/application/showtheses?thesesId=1406
https://fedorakg.kg.ac.rs/fedora/get/o:419/bdef:Content/download

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