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Fatty acids phospholipids profiles and parameters of oxidative stress in blood and liver in Wistar rats treated with fish oil

dc.contributor.advisorMandić, Ljuba
dc.contributor.otherGlibetić, Marija
dc.contributor.otherSpasić, Mihajlo
dc.contributor.otherVrvić, Miroslav
dc.contributor.otherBorozan, Sunčica
dc.creatorPopović, Tamara B.
dc.date.accessioned2016-01-05T12:59:46Z
dc.date.available2016-01-05T12:59:46Z
dc.date.available2020-07-03T10:12:57Z
dc.date.issued2012-07-10
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=266
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/3466
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:5546/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=43525647
dc.description.abstractPolinezasićene masne kiseline, posebno eikozapentaenska (EPA) i dokozaheksaenska (DHA) masna kiselina koje ulaze u sastav ribljeg ulja i pripadaju n-3 familiji masnih kiselina, imaju efekte na čitav niz fizioloških parametara. Značaj balansa odnosa n-6/n-3 masnih kiselina u fosfolipidima kao i balans izmeñu produkcije reaktivnih kiseoničnih vrsta i reaktivnih azotnih vrsta s jedne i antoksidativne zaštite s druge strane je od važnosti za normalno funkcionisanje organizma. Suplementirali smo pacove Wistar soja dve starosne dobi (3 i 22 meseca) u trajanju od 6 nedelja sa ribljim uljem (EPA+DHA i vitamin E). Ispitivani su biohemijski parametri plazme, masnokiselinski profili fosfolipida plazme i jetre kao i parametri oksidativnog stresa u krvi i jetri pomenutih životinja. Dobijeni rezultati su pokazali više vrednosti holesterola, HDL- holesterola i triglicerida u plazmi mladih pacova u odnosu na stare. Tretman ribljim uljem doveo je do povišenja HDL-holesterola, koncentracije mokraćne kiseline i sniženja LDL-holesterola kod obe grupe tretiranih životinja dok je kod mladih tretman izazvao sniženje koncentracije triglicerida a kod starih sniženje holesterola. Starenjem, povećava se sadržaj linolne, arahidonske kiseline kao i ukupnih n-6 MK. Tretman ribljim uljem, doveo je do povećanja sadržaja EPA, DPA, sniženja koncentracije arahidonske kiseline u fosfolipidima plazme u obe starosne grupe životinja. Sadržaj oleinske kiseline je kod mladih pacova tretmanom povižen, dok je kod starih pacova došlo do povećanja dihomo-γ- linolenske kiseline. Starenjem, snižava se i sadržaj ukupnih n-3 MK i odnos n-6/n-3 u fosfolipidima jetre. Tretman u životinja obe starosne dobi doveo je do povećanja sadržaja EPA, DPA, ukupnih n-3, smanjenja sadržaja arahidonske kiseline u fosfolipidima jetre. Kod mladih tretman je povećao sadržaj MUFA, a kod starih SFA i PUFA. Starenjem, povećava se lipidna peroksidacija u hepatocitima, kao i aktivnost SOD i koncentracija nitrita, dok je aktivnost PON1 u plazmi veća kod mladih pacova što je u korelaciji sa višim vrednostima HDL-holesterola u odnosu na stare pacove. Tretman ribljim uljem kod pacova obe starosne dobi doveo je do sniženja lipidne peroksidacije, koncentracije plazma nitrita i zastupljenosti LDH5 izoenzimskog oblika a do povišenja aktivnosti SOD, CAT i PON1 u krvi tretiranih životinja u odnosu na kontrolnu grupu...sr
dc.description.abstractPolyunsaturated fatty acids, especially eicosapentanoic acid (EPA) and dosahexanoic acid (DHA) which are the content of fish oil and belong to the n-3 fatty acid family. n-3 are known to have effect on physiological parameters. The balance of the n-6/n-3 ratio in phospholipids, as well as the balance between reactive oxygen species and reactive nitric species on the one side and on the anti oxidative defense on the other is important for the normal function of organism. Wistar rats (3 and 22 months) were supplemented with fish oil (EPA+DHA and vitamin E) over the period of 6 weeks. Biochemical parameters in plasma, fatty acid phospholipids profiles and the parameters of oxidative stress in blood and liver were examined. In young rats plasma concentrations of cholesterol, HDL-cholesterol and triglycerides in plasma were increased compared to aged rats. Fish oil treatment increased HDL-cholesterol, uric acid and LDL-cholesterol in both groups of animals (3 and 22 months). Treatment decreased triglycerides concentrations in young rats and decreased plasma cholesterol in aged rats. Senescence increased LA, AA and n-6 FA. Fish oil treatment increased EPA, DPA, decreased AA in plasma phospholipids in both group of animals. In young rats treatment increased oleic acid while in aged it increased DGLA. Senescence decreased n-3 FA and the n-6/n-3 ratio in liver phospholipids. Treatment increased EPA, DPA, n-3 and decreased AA in liver phospholipids in both group of animals. In young rats, treatment increased MUFA while in aged rats it increased SFA and PUFA. Senescence also increased lipid peroxidation in liver cells, as well as SOD activity and nitrite concentration while the activity of PON1 in plasma is increased in young rats compared to aged Wistar rats. Fish oil treatment in both groups of rats (3 and 22 months) decreased lipid peroxidation, concentration of plasma nitrite and LDH5 and increased SOD, CAT, PON1 activity in blood. Treatment decreased lipid peroxidation, nitrite concentration in liver of both groups of rats. In young rats treatment also decreased CAT activity and increased PON1. In aged rats treatment increased CAT, SOD activity, concentration of SH groups while PON activity decreased compared to control...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Хемијски факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectmasnokiselinski profili fosfolipidasr
dc.subjectfatty acids phospholipids profilesen
dc.subjectEPAen
dc.subjectDHAen
dc.subjectsenescenceen
dc.subjectoxidative stressen
dc.subjectEPAsr
dc.subjectDHAsr
dc.subjectstarenjesr
dc.subjectoksidativni stressr
dc.titleMasnokiselinski profili fosfolipida i parametri oksidativnog stresa u krvi i jetri pacova Wistar soja tretiranih ribljim uljemsr
dc.titleFatty acids phospholipids profiles and parameters of oxidative stress in blood and liver in Wistar rats treated with fish oilen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dcterms.abstractМандић, Љуба; Глибетић, Марија; Врвић, Мирослав; Спасић, Михајло; Борозан, Сунчица; Поповић, Тамара Б.;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/29356/Disertacija.pdf


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