Епизоотиолошка, серолошка и молекуларна истраживања врста рода Trichinella
Epizootiological, serological and molecular investigations of Trichinella species
AuthorŽivojinović, Milena Ž.
Committee membersSofronić, Ljiljana
MetadataShow full item record
У овом раду испитивана је присутност трихинела на ендемском подручју Браничева и Подунавља код домаћих и дивљих животиња, као и идентификација присутних врста рода Trichinella...
In this work we investigated presence of Trichinella species at endemic districts Branicevo and Podunavlje in population of domestic and wild animals. We examined 211 samples of muscular tissue from domestic pigs and 30 samples from dogs, by artificial digestion. Positive result was in 25 (11, 85%) samples from pigs and 7 (23,33%) dogs. Significant different (p<0, 01) was in percentage of infection in population of pigs from backyards and pigs from industrial farms. Among wild life, 338 samples were examined. Trichinella infections were detected in 7 from 174 (4,02%) wild boars (Sus scrofa), in 8 from 118 (6, 78%) foxes (Vulpes vulpes), in 8 from 42 (19, 05%) jackals (Canis aureus) and in all for examined wolves (Canis lupus). During serology survey, performed at industrial farms in 150 samples of swine sera we didn’t detect anti-Trichinella antibodies by ELISA test. In backyards we confirmed positive results in 26 (12, 68%) swine sera, weakly positive results in 6 (2, 93%) sera sample...s and 13 (6, 34%) negative results. From 11 serum samples of stray dogs from municipality of Pozarevac, 8 have specific anti-Trichinella antibodies, 1 was suspect and 2 were negative. We examined 17 samples of meet juice from wild boars when 7 samples were positive and in 10 samples we didn’t detect anti-Trichinella antibodies by ELISA test. Results from serology investigation were confirmed by artificial digestion. All positive and weakly positive samples in ELISA test (32 samples) were confirmed as positive in Western blot test. Samples examined by Multiplex PCR were from 6 animal species: 25 domestic swine, 7 wild boars, 8 foxes, 8 jackals, 4 wolves and 7 dogs. We obtained result from 53 samples and non result in 6 (10, 2%) samples. Trichinella spiralis were identified in 43 (72, 9%) samples and Trichinella britovi in 7 samples (11,8%). Mix infection with boat species were in 3 samples (5, 1%). Trichinella spiralis was found in 25 domestic pigs, 5 dogs, 5 foxes, 5 jackals and 3 wild boars. Trichinella britovi was found only in wild animals (1 wild boar, 1 jackal, 1 fox and 4 wolves). Mix infection (T. spiralis ( T. britovi) was found in 2 foxes and 1 jackal. In population of domestic pig and dogs only T. spiralis was found. Geographical distribution showed at Podunavlje district only presents of T. spiralis and at Branicevo district presents of T. spiralis ( T. britovi...
Faculty:Универзитет у Београду, Факултет ветеринарске медицине
Keywords:Trichinella spiralis / Trichinella spiralis / Trichinella britovi / domestic and wild animals / infection / epizootiology / Trichinella britovi / домаће и дивље животиње / инфекција / епизоотиологија
Showing items related by title, author, creator and subject.
Efekat ekskretorno-sekretornog antigena mišićnih larvi Trichinella spiralis i antihelmintika mebendazola na ćelije melanoma u uslovima in vitro i in vivo / The effect of excretory-secretory antigen of Trichinella spiralis muscle larvae and antihelmintic mebendazole on melanome ells in vitro and in vivo Vasilev, Saša D. (Универзитет у Београду, Факултет ветеринарске медицине, 21-12-2012)
Ispitivanje uloge komponenti ESL1 antigena Trichinella spiralis u oblikovanju imunskog odgovora Dark agouti pacova / The role of Trichinella spiralis ESL1 antigens in shaping of immune response in Dark agouti rats Cvetković, Jelena D. (Универзитет у Београду, Биолошки факултет, 09-07-2015)
Primena 7C2C5 monoklonskog antitela u razvoju ELISA testova za otkrivanje infekcije sa Trichinella spp. i izolaciju komponenti parazita koje nose imunodominantni epitop / Application of 7C2C5 monoclonal antibody in the development of ELISAs for the detection of Trichinella infection and for the isolation of parasite components that carry an immunodominant epitope Gnjatović, Marija (Универзитет у Београду, Технолошко-металуршки факултет, 24-12-2018)