Епизоотиолошка, серолошка и молекуларна истраживања врста рода Trichinella
Epizootiological, serological and molecular investigations of Trichinella species
Author
Živojinović, Milena Ž.Mentor
Kulišić, Zoran
Committee members
Sofronić, LjiljanaTeodorović, Vlado

Radojičić, Sonja

Mirilović, Milorad

Metadata
Show full item recordAbstract
У овом раду испитивана је присутност трихинела на ендемском подручју Браничева и Подунавља код домаћих и дивљих животиња, као и идентификација присутних врста рода Trichinella...
In this work we investigated presence of Trichinella species at endemic districts
Branicevo and Podunavlje in population of domestic and wild animals. We examined
211 samples of muscular tissue from domestic pigs and 30 samples from dogs, by
artificial digestion. Positive result was in 25 (11, 85%) samples from pigs and 7
(23,33%) dogs. Significant different (p<0, 01) was in percentage of infection in
population of pigs from backyards and pigs from industrial farms. Among wild life, 338
samples were examined. Trichinella infections were detected in 7 from 174 (4,02%)
wild boars (Sus scrofa), in 8 from 118 (6, 78%) foxes (Vulpes vulpes), in 8 from 42 (19,
05%) jackals (Canis aureus) and in all for examined wolves (Canis lupus).
During serology survey, performed at industrial farms in 150 samples of swine
sera we didn’t detect anti-Trichinella antibodies by ELISA test. In backyards we
confirmed positive results in 26 (12, 68%) swine sera, weakly positive results in 6 (2,
93%) sera sample...s and 13 (6, 34%) negative results. From 11 serum samples of stray
dogs from municipality of Pozarevac, 8 have specific anti-Trichinella antibodies, 1 was
suspect and 2 were negative.
We examined 17 samples of meet juice from wild boars when 7 samples were
positive and in 10 samples we didn’t detect anti-Trichinella antibodies by ELISA test.
Results from serology investigation were confirmed by artificial digestion. All positive
and weakly positive samples in ELISA test (32 samples) were confirmed as positive in
Western blot test.
Samples examined by Multiplex PCR were from 6 animal species: 25 domestic
swine, 7 wild boars, 8 foxes, 8 jackals, 4 wolves and 7 dogs. We obtained result from 53
samples and non result in 6 (10, 2%) samples. Trichinella spiralis were identified in 43
(72, 9%) samples and Trichinella britovi in 7 samples (11,8%). Mix infection with boat
species were in 3 samples (5, 1%). Trichinella spiralis was found in 25 domestic pigs, 5
dogs, 5 foxes, 5 jackals and 3 wild boars. Trichinella britovi was found only in wild
animals (1 wild boar, 1 jackal, 1 fox and 4 wolves). Mix infection (T. spiralis ( T.
britovi) was found in 2 foxes and 1 jackal. In population of domestic pig and dogs only T. spiralis was found. Geographical distribution showed at Podunavlje district only
presents of T. spiralis and at Branicevo district presents of T. spiralis ( T. britovi...
Faculty:
Универзитет у Београду, Факултет ветеринарске медицинеDate:
25-12-2013Keywords:
Trichinella spiralis / Trichinella spiralis / Trichinella britovi / domestic and wild animals / infection / epizootiology / Trichinella britovi / домаће и дивље животиње / инфекција / епизоотиологијаRelated items
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