Morfološke, imunofenotipske i kliničke karakteristike limfocitno-plazmocitnog duodenitisa pasa

Abstract

Limfocitno – plazmocitni duodenitis (LPD) pasa je oblik inflamatorne bolesti creva. LPD predstavlja enteropatiju hroničnog toka i praćen je nespecifičnim kliničkim simptomima, od kojih su najvažniji dijareja i povraćanje. Najvažnija morfološka karakteristika imunološkog odgovora kod limfocitno - plazmocitnog duodenitisa (LPD) pasa je nalaz mononuklearnog ćelijskog infiltrata u sluznici duodenuma. Nakon dobro obavljenog kliničkog pregleda i detaljne anamneze, može se pretpostaviti moguće mesto inflamacije. Prikupljanje preciznih podataka, uzimanje sveobuhvatne anamneze i vršenje detaljnog kliničkog pregleda pasa je od neprocenjivog značaja za pravilno postavljanje dijagnoze. Klinički znaci vezani za LPD nisu specifični. Imaju cikličan karakter, reĎe progresivan klinički tok. Najčešći simptomi uključuju hroničnu ili rekurentnu dijareju, povraćanje, gubitak težine i promenu apetita. Ispitivanjem je obuhvaćeno ukupno 60 odraslih pasa, različite rase i oba pola: 54 psa sa simptomima dijareje i povraćanja, koje je trajalo duže od tri nedelje, kao i šest klinički zdravih pasa koji su služili kao kontrolna grupa. Ispitivanja su obuhvatila pse koji su bili pacijenti ambulantne klinike Fakulteta veterinarske medicine (FVM) u Beogradu. Prema anamnestičkim podacima, svi psi su pre dolaska u ambulantu FVM bili neuspešno lečeni antibioticima i svi su bili slobodni od crevnih parazita. UraĎene su hematološke i biohemijske analize krvi, zatim radiološka, ultrazvučna i endoskopska dijagnostika. Uzorci mukoze duodenuma, uzeti biopsijom, ispitani su patohistološkim i imunohistohemijskim metodama. Analizom rezultata dobijenih u ovom istraživanju, ustanovljeno je da oboleli psi, koji su u kliničkoj slici imali hroničnu dijareju, hronično povraćanje, izmenjeno ponašanje i promenjen apetit, boluju od sledećih enteropatija: kataralni duodenitis je utvrĎen kod 5 pasa, kod 3 psa je postavljena dijagnoza eozinofilnog duodenitisa dok je kod 46 pasa dijagnostikovan limfocitno – plazmocitni duodenitis (LPD)...

Lymphocytic - plasmocytic duodenitis (LPD) in dogs is a form of chronic inflammatory bowel disease. LPD is a chronic enteropahty accompanied with non specific clinical symptoms out of which diarrhea and vomiting are the most significant. The most important morphological feature of the immune response in this type of inflammatory bowel disease is the finding of mononuclear infiltrates in the mucosa of the duodenum. After thorough clinical examination and detailed patient’s history the inflammation site can be described. Gathering of precise data, history, and a detailed clinical examination are of the upmost importance for the diagnosis. Clinical signs of LPD are not specific. They have a cyclic character and a seldom progressive clinical course. The most common symptoms include chronic or recurrent diarrhea, vomiting, weight loss, and changes in appetite. The study involved 60 adult dogs of different breeds and both sexes: 54 dogs with symptoms of diarrhea and vomiting that persisted longer than three weeks and six clinically healthy dogs that served as controls. The investigation included dogs which were patients at the ambulatory clinic at the Faculty of Veterinary Medicine, Belgrade (Serbia). According to anamnestic data all dogs were previously unsuccessfully treated with antibiotics and were free of intestinal parasites. Hematological and biochemical blood tests, radiology, ultrasonography and endoscopy tests were carried out. Biopsy samples of the duodenal mucosa were investigated with pathohistological and immunohistochemical methods. By analysis of the results obtained in this study it was shown that dogs, which in the clinical picture had chronic diarrhea, chronic vomiting, abnormal behavior and altered appetite, suffered from the following enteropathies: catarrhal duodenitis was found in 5 dogs, 3 dogs were diagnosed with eosinophilic duodenitis and 46 dogs were diagnosed with lymphocytic - plasmocytic duodenitis (LPD)...