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Detekcija i tkivna distribucija nukleinske kiseline i Cap antigena cirkovirusa-2 kod domaćih i divljih svinja

Detection and tissue distribution of the nucleic acid and Cap antigen of porcine circovirus type 2 in domestic swine and wild boars

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2012
Disertacija.pdf (15.65Mb)
Author
Bečkei, Žolt
Mentor
Knežević, Milijana
Committee members
Aleksić-Kovačević, Sanja
Ivetić, Vojin
Petrović, Tamaš
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Abstract
Srbiji cirkovirusne infekcije su nedovoljno istražene. Postoje oskudni podaci o prisustvu PCV2 u pojedinim zpatima, ali je veoma malo saznanja o prisustvu PCVAD (oboljenja koja su u uzročnoj vezi sa PCV2) u zapatima domaćih svinja. Nema podataka o prevalenciji cirkovirusnih infekcija i o prisustvu PCVAD kod divljih svinja. Od 2011. godine u Srbiji registrovana je i komercijalno dostupna vakcina, te je imunoprofilaksa od nedavno jedna od mogućih mera u prevenciji cirkovirusnih oboljenja. Stoga su u ovom radu ispitani limfatični i nelimfatični organi 60 domaćih svinja, sa kliničkim znacima sistemskog oblika PCVAD, tj. multisistemskog sindroma kržljanja zalučene prasadi (PMWS), uzrasta od jedan do pet meseci, koja su poticala sa osam farmi različitog lokaliteta epizootiološkog područja Severno-bačkog okruga; zatim 60 divljih svinja različite starosti, odstreljenih u dva lovišta na epizootiološkom području Severno-bačkog okruga, u periodu 2005-2010. godine; i po 10 zdravih domaćih i divlji...h svinja koje su činile kontrolnu grupu. Za histopatološku, imunohistohemijsku analizu i hibridizaciju in situ od domaćih i divljih svinja uzorkovani su limfatični organi (submandibularni, medijastinalni, mezenterijalni i ingvinalni superficijalni limfni čvorovi, tonzile, slezina i limfatično tkivo creva), a od nelimfatičnih organa pluća, srce, jetra i bubrezi...

Porcine circovirus associated diseases (PCVAD) are widespread throughout the world and cause significant economic losses in intensive swine production. The causative agent playing a key role in their pathogenesis is porcine circovirus type 2 (PCV2). Circovirus infections are insufficiently explored in Serbia. There is information about the presence of PCV2 in some swine herds, but very little knowledge exists about the presence and extent of PCVAD in domestic swine herds, and no data are available on the prevalence and incidence of PCV2 infection in wild boars. Since 2011, a vaccine against PCV2 infection has been registered and commercially available in Serbia. Thus, immunoprophylaxis is one of the possible measures to prevent the diseases caused by circovirus. Therefore, the lymphatic and non-lymphatic organs of 60 domestic swine aged one to five months and showing clinical signs of postweaning multisystemic wasting syndrome (PMWS) were examined in this study. The pigs originated fro...m eight different farms in the epizootiological area of North Bačka County. We also examined 60 wild boars of different ages, sacrificed in two hunting area of the North Bačka County in the period 2005–2010, as well as 10 healthy domestic swine and 10 healty wild boars that comprised the control group...

Faculty:
University of Belgrade, Faculty of Veterinary Medicine
Date:
26-11-2012
Keywords:
PCVAD / PCVAD / PMWS / PCV2 / Cap antigen / jednolančana DNK PCV2 / dvolančana DNK PCV2 / imunohistohemija / hibridizacija in situ / divlje svinje / domaće svinje. / PMWS / PCV2 / Cap antigen / ssDNA PCV2 / dsDNA PCV2 / immunohistochemistry / in situ hybridisation / wild boar / domestic swine
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URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=534
http://nardus.mpn.gov.rs/handle/123456789/2956
https://fedorabg.bg.ac.rs/fedora/get/o:6556/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=42747919

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