Značaj vaskularnog endotelnog faktora rasta i kostnog morfogenetskog proteina-2 za reparatorne sposobnosti zdrave i dijabetesom izmenjene zubne pulpe
The significance of vascular endothelial growth factor and bone morphogenetic protein-2 in reparative abilities of healthy and diabetic dental pulp
Author
Ilić, Jugoslav
Mentor
Stojić, DragicaCommittee members
Vujašković, MirjanaPudar-Branković, Georgina
Petrović, Ljubomir

Metadata
Show full item recordAbstract
Zubna pulpa je vezivno tkivo koje sa dentinom, koji je okružuje čini jedinstvenu
embriološku i anatomsko-fiziolšku celinu koja se uobičajno naziva pulpodentinski
kompleks. Reakcije pulpe, uključujući i reparatorne procese, razlikuju se u osnovi od
reakcije drugih vezivnih tkiva zbog njene specifične lokalizacije unutar čvrstih
dentinskih zidova, kao i jedinstvenog vaskularnog sistema. Početak, vrsta i intenzitet
reparatornog odgovora pulpe zavisi od niza ćelijskih procesa kao što su stimulacija
sekrecije odontoblasta, specifična citodiferencijacija (odontoblasti, endotelijalne ćelije),
kao i neovaskularizacija na mestu povrede. Signalni molekuli uključeni u transdukcione
mehanizme ovih procesa su tkivni faktori rasta od kojih su od posebne važnosti koštani
morfogenetski protein 2 (BMP-2) i faktor rasta vaskularnog endotela (VEGF). Povećana
ekspresija BMP-2 je nađena u ćelijama pulpe tokom tercijerne dentinogeneze i utvrđena
je uloga ovog faktora rasta na diferencijaciju specifičnih ćel...ija pulpe neophodnih u
reparatornom odgovoru. Proces neovaskularizacije, regulisan aktivnošću VEGF,
kritičan je za adekvatan pulpni reparativni odgovor. Diabetes mellitus (DM) je
rasprostranjeno oboljenje koje dovodi do brojnih metaboličkih poremećaja sa
povećanjem oksidativnog stresa u tkivima. Značajne posledice ovog patofiziološkog procesa su i promene u tkivnim koncentracijama raznih faktora rasta, posebno VEGF ali
i BMP-2. Imajući u vidu značaj BMP-2 i VEGF u procesima reparacije dentina u
zdravim zubima, kao i činjenicu da dijabetes u mnogim tkivima menja funkciju ovih
faktora rasta, postavili smo hipotezu da dijabetes značajno menja nivo i ulogu BMP-2 i
VEGF u reparatornim odgovorima pulpe...
Dental pulp is a connective tissue which makes embryologic, anatomic and
functional entity with surrounding dentin referred to as the pulp-dentin complex. Due to
specific localization within solid dentinal walls, as well as unique vasculature, pulp
reactions significantly differ to reactions of other connective tissues. Commence, type
and intensity of pulp reparative response depend on cellular events including upregulation
of synthetic and secretory activity of odontoblasts, specific
cytodifferentiation, and neovascularization at the injury site. The signaling molecules
involved in induction and regulation of these events are growth factors including very
important bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth
factor (VEGF). BMP-2 expression was found to be increased during tertiary
dentinogenesis, and significant role of this growth factor in differentiation of cells
involved in pulp reparative response was established. Neovascularization, regulated by
VEGF act...ivity, was found to be critical for pulp reparative reaction. Diabetes mellitus
(DM) is wide spread disease causing metabolic alterations as a result of increased
oxidative stress in tissues. Significant consequence of this pathogenic mechanism is
alteration in tissue levels of growth factors, particularly VEGF, but also BMP-2. Considering the importance of BMP-2 and VEGF in processes of dentinal reparation,
and the fact that DM alters the function of these growth factors in various tissues, the
hypothesis of this study was that DM significantly alters the level and role of BMP-2
and VEGF in pulp reparative responses...