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Morphological, molecular and pathogenic characterization of species Diaporthe/Phomosis on soybean in Serbia

dc.contributor.advisorIvanović, Mirko
dc.contributor.otherVidić, Miloš
dc.contributor.otherKrstić, Branka
dc.contributor.otherĐorđević, Vuk
dc.contributor.otherBulajić, Aleksandra
dc.creatorPetrović, Kristina P.
dc.date.accessioned2016-01-05T12:08:57Z
dc.date.available2016-01-05T12:08:57Z
dc.date.available2020-07-03T08:59:01Z
dc.date.issued2012
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=497
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/2542
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:6454/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=512029917
dc.description.abstractEndofitne vrste roda Diaporthe/Phomopsis imaju izuzetno širok spektar domaćina i u biljkama mogu biti prisutne kao patogeni ili saprofiti. Na stablu i semenu soje (Glycine max (L.) Merr.), vrste ovog roda prouzrokuju kompleks oboljenja i spadaju u grupu značajnih parazita ove biljne vrste. Kao paraziti soje opisane su vrste Diaporthe phaseolorum i Phomopsis longicolla. U okviru prvo pomenute vrste razlikuju se tri varijeteta, i to: D. phaseolorum var. sojae, D. phaseolorum var. caulivora i D. phaseolorum var. meridionalis. Iako su u mnogobrojnim istraživanjima detaljno opisane morfološke i odgajivačke karakteristike ovih patogena, njihova taksonomija još uvek nije u potpunosti definisana. Najveća taksonomska nedoumica odnosi se na D. phaseolorum var. sojae, jer je u okviru ovog varijeteta uočena izražena varijabilnost morfoloških karakteristika. S obzirom da je morfološka varijabilnost D. phaseolorum var. sojae uočena i kod izolata poreklom iz Srbije, ukazala se potreba za detaljnim ispitivanjem, kako bi se tačno utvrdile prisutne Diaporthe/Phomopsis vrste na soji u Srbiji. Uporednim proučavanjem morfoloških, molekularnih i patogenih karakteristika, identifikovano je ukupno sedam vrsta roda Diaporthe/Phomopsis. Pored poznatih parazita, P. longicolla, D. phaseolorum var. sojae i D. phaseolorum var. caulivora, na semenu soje su detektovane i vrste D. neotheicola, D. eres, D. viticola i neidentifikovana grupa Phomopsis sp. 9. Identifikacija i filogenetsko grupisanje Diaporthe/Phomopsis vrsta sprovedeno je na osnovu sekvenci ITS regiona i dela LSU i TEF1-α gena. U cilju iznalaženja bržeg načina detekcije i identifikacije, ITS sekvence proučavanih vrsta poslužile su za virtuelnu digestiju restrikcionim enzimima AluI, RsaI, HhaI, MseI i StyI. S obzirom da su za svaku proučavanu vrstu dobijeni različiti molekularni profili, konstatovano je da PCRRFLP tehnika potencijalno može biti korisna metoda za brzu i pouzdanu identifikaciju Diaporthe/Phomopsis vrsta koje se javljaju na soji. Proučavanjem morfoloških karakteristika identifikovanih vrsta, utvrđeno je da se dimenzije reproduktivnih organa preklapaju, ali i da veoma variraju između izolata unutar jedne vrste, kao i unutar samog izolata. Na osnovu toga, izveden je zaključak da dimenzije reproduktivnih organa nisu pouzdani pokazatelji pri identifikaciji Diaporthe/Phomopsis vrsta izolovanih iz soje...sr
dc.description.abstractSpecies of the genus Diaporthe/Phomopsis are endophytes on a very wide range of hosts and can be present as pathogens or saprophytes. Species of this genus cause a complex of diseases on the stem and seeds of soybean (Glycine max (L.) Merr.) and therefore belong to a group of important parasites of this plant. Diaporthe phaseolorum and Phomopsis longicolla were described as soybean pathogens. The first species includes three varieties: D. phaseolorum var. sojae, D. phaseolorum var. caulivora and D. phaseolorum var. meridionalis. Although several studies have extensively described the morphological and culture characteristics of these pathogens, their taxonomy has not fully been defined. The greatest taxonomic confusion is related to D. phaseolorum var. sojae, because this variety expressed variability of morphological characteristics. Since morphological variability of D. phaseolorum var. sojae was also observed in isolates from Serbia, there were needs for detailed examinations in order to exactly determine which Diaporthe/Phomopsis species are present on soybean in Serbia. By comparison of morphological, molecular and pathogenic data seven Diaporthe/Phomopsis species were identified. Besides the known parasites, D. neotheicola, D. eres, D. viticola and unidentified group Phomopsis sp. 9 were also detected on soybean seeds. Identification and phylogenetic grouping was performed based on sequences of ITS region and parts of LSU and TEF1-α genes. In order to find faster ways of detection and identification, ITS sequences of the studied species were used for the virtual digestion with restriction enzymes AluI, RsaI, HhaI, MseI, and StyI. Since different molecular profiles were generated for each studied species, it was concluded that PCR-RFLP technique can potentially be a useful method for rapid and reliable identification of Diaporthe/Phomopsis species that occur on soybean. By studying morphological characteristics of the identified species, it was found that the dimensions of reproductive organs overlapping, but also that they vary between isolates within a species, as well as within the isolates. Based on this, it was concluded that dimensions of reproductive organs are not reliable parameters for identification of Diaporthe/Phomopsis species isolated from soybeans...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Пољопривредни факултет
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31022/RS//
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectsojasr
dc.subjectsoybeanen
dc.subjectDiaporthe/Phomopsissr
dc.subjectITSsr
dc.subjectLSUsr
dc.subjectTEF1-αsr
dc.subjectfilogenijasr
dc.subjectPCR-RFLPsr
dc.subjectmorfologijasr
dc.subjectpatogenostsr
dc.subjecttaksonomijasr
dc.subjectDiaporthe/Phomopsisen
dc.subjectITSen
dc.subjectLSUen
dc.subjectTEF1-αen
dc.subjectphylogenyen
dc.subjectPCRRFLPen
dc.subjectmorphologyen
dc.subjectpathogenicityen
dc.subjecttaxonomyen
dc.titleMorfološka, molekularna i patogena karakterizacija vrsta Diaporthe/Phomopsis na soji u Srbijisr
dc.titleMorphological, molecular and pathogenic characterization of species Diaporthe/Phomosis on soybean in Serbiaen
dc.typedoctoralThesisen
dc.rights.licenseBY-NC-ND
dcterms.abstractИвановић, Мирко; Ђорђевић, Вук; Булајић, Aлександра; Видић, Милош; Крстић, Бранка; Петровић, Кристина П.;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/12478/Disertacija.pdf


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