Show simple item record

Analysis of activity of antioxidative enzymes, matrix metaloproteinases-2 and -9 and cell death parameters in tissue of breast cancer patients

dc.contributor.advisorGopčević, Kristina
dc.contributor.otherKonjević, Gordana
dc.contributor.otherIsaković, Aleksandra
dc.contributor.otherMilošević, Zorica
dc.contributor.otherJurišić, Vladimir
dc.creatorRadenković, Sandra S.
dc.date.accessioned2016-01-05T12:05:46Z
dc.date.available2016-01-05T12:05:46Z
dc.date.available2020-07-03T08:53:36Z
dc.date.issued2013-06-06
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=724
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/2383
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:6994/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44670735
dc.description.abstractUvod: Obzirom da je aktivnost laktat dehidrogenaze (LDH) i malat dehidrogenaze (MDH) povezana sa procesima proliferacije i invazije tumora, odredjivanje distribucije njihovih izoenzima važno je u procesu metabolizma tumorske ćelije. Dugo se smatralo da enzimi antioksidativne zaštite, kao što su superoksid dismutaza (SOD) i katalaza (CAT), štite ćelije od kancerogeneze. Medjutim, pokazano je da su CAT i SOD uključene u procese izbegavanja ćelijske smrti i proliferacije, zbog čega predstavljaju važne medijatore u biologiji karcinoma dojke. Sve češće povezivanje mamografskih karakteristika sa histopatološkim markerima i enzimima, stvara novu klasifikaciju pacijentkinja sa karcinomom dojke u cilju adekvatnog individualnog pristupa. Pored povećane aktivnosti enzima antioksidativne zaštite, u karcinomima dolazi do poremećaja ekspresije pokazatelja ćelijske smrti kao što su STAT3, ciklin D1 i Bcl-2. Obzirom na porast incidence karcinoma dojke u svetu, javlja se sve veća potreba za definisanjem panela tumorskih markera koji bi pouzdano mogli da identifikuju karcinom dojke sa mogućom tendencom recidiva. Podaci pokazuju da marker za karcinom dojke može biti medju proteinima koji učestvuju u procesu tumorske progresije kao što su MMP-2, MMP-9 i TIMP1. Cilj: Iz tih razloga, cilj ovog rada je bio utvrdjivanje aktivnosti enzima antioksidativne zaštite (SOD, CAT), anaerobnog metabolizma (LDH, MDH), invazivnosti (MMP-2, MMP-9, TIMP1) i ekspresije pokazatelja ćelijske smrti (pSTAT3, bcl-2, Ciklin D1) u tumorskom tkivu i njihovog odnosa sa kliničko-patološkim karakteristikama tumora, kao i sa mamografskim nalazom bolesnica sa karcinomom dojke. Materijal i metode: U radu je analizirano 75 uzoraka tumorskog i 75 uzoraka okolnog peritumorskog tkiva bolesnica sa histopatološki potvrđenim karcinomom dojke. Aktivnost MMP-2 i MMP-9 određivana je metodom želatin zimografije, dok je aktivnost SOD, CAT, LDH i MDH određivana odgovarajućim spektrofotometrijskim metodama...sr
dc.description.abstractIntroduction: Lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) activity is associated with highly invasive and metastatic breast cancer. It was long thought that the antioxidative enzymes such as superoxide dismutase (SOD) and catalase (CAT), protect cells against carcinogenesis. However, it has been shown that SOD and CAT play role in processes of proliferation and cell death. Association of enzyme profile and mammographic features provide insights into the biological processes in breast cancer and could be of use in defining novel biomarkers and potential therapeutic targets. Together with increased activities of antioxidative enzymes, cancers have altered expression of cell death parameters such as STAT3, Bcl-2 and cyclin D1. Since the incidence of breast cancer is increasing, it is necessary to find more tumor markers for breast cancer patients with high risk of recurrence. The proteins of tumor progression such as MMP-2, MMP-9 and TIMP1 could be possible tumor markers. Aim: The aim of this study was to examine the activity of antioxidative (SOD, CAT), anaerobic metabolism (LDH, MDH), invasivness (MMP-2, MMP-9) enzymes and expression of cell death parameters (TIMP1, STAT3, cyclin D1, Bcl-2) and their relation with clinico-pathologic and mammographic characteristics of breast cancer patients. Material and methods: In this study we analyzed tumor and adjacent tissue samples of 75 breast cancer patients. We examined the activity of MMP-2 and MMP-9 by gelatin zymography, while SOD, CAT, LDH and MDH activity was assayed spectrophotometrically. Distribution of isoforms was determined with direct and reverse electrophoretic zymography. Expression of TIMP1, STAT3, cyclin D1 and Bcl-2 was assessed in tumor lysates by Western blotting. Mammograms were evaluated according to the American College of Radiology Breast Imaging Reporting and Data system (BIRADS). Significance of differences of the obtained results was analyzed by the T test, Wilcoxon rank sum test and Mann–Whitney U-test. Results: The LDH5 izoenzyme was detected in tumor tissue of breast cancer patients. The activity of MDH, m-MDH, CAT and m-SOD in tumor tissue of breast cancer patients significantly increases compared to adjacent patient tissue...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Медицински факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175056/RS//
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectkarcinom dojkesr
dc.subjectbreast canceren
dc.subjectantoksidativni enzimisr
dc.subjectželatinazesr
dc.subjectpokazatelji ćelijske smrtisr
dc.subjectantioxidative enzymesen
dc.subjectgelatinasesen
dc.subjectcell death parametersen
dc.titleIspitivanje aktivnosti enzima antioksidativne zaštite, matriks metaloproteinaza-2 i -9 i pokazatelja ćelijske smrti u tkivu pacijentkinja sa karcinomom dojkesr
dc.titleAnalysis of activity of antioxidative enzymes, matrix metaloproteinases-2 and -9 and cell death parameters in tissue of breast cancer patientsen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dcterms.abstractГопчевић, Кристина; Коњевић, Гордана; Исаковић, Aлександра; Јуришић, Владимир; Милошевић, Зорица; Раденковић, Сандра С.; Испитивање активности ензима антиоксидативне заштите, матрикс металопротеиназа-2 и -9 и показатеља ћелијске смрти у ткиву пацијенткиња са карциномом дојке; Испитивање активности ензима антиоксидативне заштите, матрикс металопротеиназа-2 и -9 и показатеља ћелијске смрти у ткиву пацијенткиња са карциномом дојке;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/10943/Disertacija.pdf


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record