Fenotipske karakteristike glatkih mišićnih ćelija u arterijama osoba sa uznapredovalim formama ateroskleroze

Abstract

Predmet ovog istraživanja je evaluacija uloge citohistoloških faktora koji doprinose patogenezi ateroskleroze. Istraživanje će obuhvatiti karakteristike različitih faza ateroskleroze, citohistološku i imunohistohemijsku analizu aterosklerotičnih promena, kao i utvrđivanje moguće povezanosti drugih faktora sa kliničkom slikom pacijenata. Planirana je retrospektivna kliničko-histopatološka studija tipa slučaj-kontrola. Studija će obuhvatiti 21 uzorak u grupi slučajeva i 41 uzorak u grupi kontrola. Grupu slučajeva čine uzorci koronarnih arterija umrlih, sa uznapredovalim formama ateroskleroze. Grupu kontrola čine uzorci koronarnih arterija umrlih, sa ranim lezijama ateroskleroze. U studiji će biti analizirane citohistološke karakteristike stadijuma ateroskleroze prema klasifikaciji American Heart Association Committee on Vascular Lesions of the Council of Atherosclerosis, imunohistohemijske karakteristike korišćenjem četiri imunohistohemijska markera – α-glatkomišićni aktin, S-100 protein, dezmin i vimentin, kao i veći broj varijabli dobijenih iz istorija bolesti. Kao najvažniji rezultat ove studije očekuje se utvrđivanje najznačajnijih citohistoloških faktora koji vode progresiji ateroskleroze koronarnih arterija. Osim toga, rezultati imunohistohemijske analize u ovoj studiji mogli bi da ukažu na povezanost kliničke slike koronarne bolesti i njenih citohistoloških karakteristika, odnosno, stadijuma ateroskleroze.

The subject of this research is the evaluation of the role of cytohistological factors that contribute to the pathogenesis of atherosclerosis. The research will include the characteristics of different stages of atherosclerosis, cytohistological and immunohistochemical analysis of atherosclerotic changes, as well as determining the possible association of other factors with the clinical picture of patients. A retrospective clinical-histopathological case-control study has been planned. The study will include 21 samples in the case group and 41 samples in the control group. The case group consists of samples of coronary arteries of deceased persons with advanced types of atherosclerosis. The control group consists of samples of coronary arteries of deceased persons with early lesions of atherosclerosis. The study will analyze the cytohistological characteristics of the stage of atherosclerosis according to the classification of the American Heart Association Committee on Vascular Lesions of the Council of Atherosclerosis, immunohistochemical characteristics by using four immunohistochemical markers - α-smooth muscle actin, S-100 protein, desmin and vimentin, as well as a larger number of variables obtained from medical histories. The determination of the most significant cytohistological factors that lead to the progression of coronary artery atherosclerosis is expected to be the most important result of this study. In addition, the results of the immunohistochemical analysis in this study could indicate the association between the clinical picture of coronary disease and its cytohistological characteristics, that is, the stage of atherosclerosis.