Fenotipske karakteristike glatkih mišićnih ćelija u arterijama osoba sa uznapredovalim formama ateroskleroze
Phenotypic characteristics of smooth muscle cells in the arteries of persons with advanced types of aterosclerosis
Докторанд
Rosić, VesnaМентор
Tanasković, IrenaЧланови комисије
Stanković, VesnaSazdanović, Maja
Milovanović, Jelena
Labudović Borović, Milica
Метаподаци
Приказ свих података о дисертацијиСажетак
Predmet ovog istraživanja je evaluacija uloge citohistoloških faktora koji doprinose
patogenezi ateroskleroze. Istraživanje će obuhvatiti karakteristike različitih faza
ateroskleroze, citohistološku i imunohistohemijsku analizu aterosklerotičnih
promena, kao i utvrđivanje moguće povezanosti drugih faktora sa kliničkom slikom
pacijenata.
Planirana je retrospektivna kliničko-histopatološka studija tipa slučaj-kontrola.
Studija će obuhvatiti 21 uzorak u grupi slučajeva i 41 uzorak u grupi kontrola. Grupu
slučajeva čine uzorci koronarnih arterija umrlih, sa uznapredovalim formama
ateroskleroze. Grupu kontrola čine uzorci koronarnih arterija umrlih, sa ranim
lezijama ateroskleroze.
U studiji će biti analizirane citohistološke karakteristike stadijuma ateroskleroze
prema klasifikaciji American Heart Association Committee on Vascular Lesions of the
Council of Atherosclerosis, imunohistohemijske karakteristike korišćenjem četiri
imunohistohemijska markera – α-glatkomišićni aktin, S-100 protein..., dezmin i
vimentin, kao i veći broj varijabli dobijenih iz istorija bolesti.
Kao najvažniji rezultat ove studije očekuje se utvrđivanje najznačajnijih
citohistoloških faktora koji vode progresiji ateroskleroze koronarnih arterija. Osim
toga, rezultati imunohistohemijske analize u ovoj studiji mogli bi da ukažu na
povezanost kliničke slike koronarne bolesti i njenih citohistoloških karakteristika,
odnosno, stadijuma ateroskleroze.
The subject of this research is the evaluation of the role of cytohistological factors that contribute
to the pathogenesis of atherosclerosis. The research will include the characteristics of different
stages of atherosclerosis, cytohistological and immunohistochemical analysis of atherosclerotic
changes, as well as determining the possible association of other factors with the clinical picture
of patients.
A retrospective clinical-histopathological case-control study has been planned. The study will
include 21 samples in the case group and 41 samples in the control group. The case group
consists of samples of coronary arteries of deceased persons with advanced types of
atherosclerosis. The control group consists of samples of coronary arteries of deceased persons
with early lesions of atherosclerosis.
The study will analyze the cytohistological characteristics of the stage of atherosclerosis
according to the classification of the American Heart Association Committee on Vascular Lesion...s
of the Council of Atherosclerosis, immunohistochemical characteristics by using four
immunohistochemical markers - α-smooth muscle actin, S-100 protein, desmin and vimentin, as
well as a larger number of variables obtained from medical histories.
The determination of the most significant cytohistological factors that lead to the progression of
coronary artery atherosclerosis is expected to be the most important result of this study. In
addition, the results of the immunohistochemical analysis in this study could indicate the
association between the clinical picture of coronary disease and its cytohistological
characteristics, that is, the stage of atherosclerosis.