Monitoring, identifikacija i molekularno-genetička karakterizacija fitopatogenih bakterija krompira (Solanum tuberosum L.)
Monitoring, identification, and molecular-genetic characterization of phytopathogenic potato bacteria (Solanum tuberosum L.)
Докторанд
Marković, Sanja S.Ментор
Popović Milovanović, TatjanaЧланови комисије
Stanković, SlavišaBerić, Tanja
Medić, Olja
Iličić, Renata
Метаподаци
Приказ свих података о дисертацијиСажетак
U Srbiji krompir ima veliki privredni značaj i pripada vodećim ratarsko-povrtarskim kulturama.
Tokom monitoringa sprovedenog od 2015 do 2019. godine, obuhvaćeno je preko 2000 ha useva
krompira sa 16 lokaliteta. Rezultati monitoringa su ukazali na prisustvo dve bakteriozne bolesti. Mrka
trulež krtola krompira utvrđena je u 2015, 2016. i 2018. godini i kao uzročnik je identifikovana
karantinska bakterija Ralstonia solanacearum. „Crna noga“ i vlažna trulež krtola krompira, koju su
prouzrokovale bakterije Pectobacterium carotovorum, Pectobacterium brasiliense i Dickeya
dianthicola, detektovana je u 2016, 2018. i 2019. godini. U ovom istraživanju, prisustvo P. brasiliense
i D. dianthicola prvi put je zabeleženo na krompiru u Srbiji. Izolati R. solanacearum dobijeni iz 229
uzoraka sa mrkom truleži krtola bili su fenotipski homogeni, a na osnovu molekularno-genetičke
karakterizacije svrstani su u filotip II. Rezultati ispitivanja fenotipskih karakteristika izolata iz 50
uzoraka sa crnom nogom... i vlažnom truleži krtola ukazali su na postojanje uzročnika iz dva roda,
Pectobacterium i Dickeya. Međutim, molekularno-genetička karakterizacija ovih izolata ukazala je
na prisustvo tri vrste, pri čemu su P. brasiliense izolati bili heterogeni, dok su P. carotovorum i D.
dianthicola izolati uglavnom bili homogeni. Metabarkoding analiza omogućila je uvid u sastav
bakterijske mikrobijalne zajednice krtola krompira sa i bez simptoma vlažne truleži, kao i
odgovarajućih zemljišta ukazujući da razvoj vlažne truleži nastaje zbog složenih interakcija između
biljnih patogena i drugih endofitnih bakterija. Biokontrolna aktivnost Bacillus amyloliquefaciens
sojeva SS-12.6 i SS-38.4 prema izolovanim fitopatogenim bakterijama je dokazana u in vitro, in situ
i in planta uslovima.
n Serbia, the potato has great economic importance and belongs to the leading crops. During the
five-year monitoring (2015-2019), over 2000 ha of potato crops from 16 locations were observed.
Monitoring results indicated the presence of two bacterial diseases. Brown rot of tubers was observed
in 2015, 2016, and 2018, caused by the quarantine bacterium Ralstonia solanacearum. Blackleg and
soft rot caused by the Pectobacterium carotovorum, P. brasiliense, and Dickeya dianthicola was
observed in 2016, 2018, and 2019. In this study, P. brasiliense and D. dianthicola was recorded for
the first time on the potato in Serbia. Isolates of R. solanacearum obtained from 229 samples with
brown rot were phenotypically homogeneous, and based on molecular-genetic characterization they
were classified into phylotype II. The results of testing the phenotypic characteristics of isolates from
50 samples with blackleg and soft rot indicated the presence of pathogens from two genera,
Pectobacterium and Dic...keya. However, molecular-genetic characterization of these isolates revealed
the presence of three species, whereby P. brasiliense isolates were heterogeneous, while P.
carotovorum and D. dianthicola isolates were mainly homogeneous. The metabarcoding analysis
provided insight into the bacterial microbial community composition of potato tubers with and
without soft rot symptoms, as well as the corresponding soils, indicating that the development of soft
rot process arises due to complex interactions between plant pathogens and other endophytic bacteria.
The biocontrol activity of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 against isolated
bacteria was proven in vitro, in situ, and in planta.