Odlučili smo se za istraživanje pojave epileptičnih napada kod pacova, izazvanih etarskim uljem kamforovog
drveta ili njegovim glavnim konstituentom 1,8-sineolom. Eksperimenti su izvedeni na odraslim pacovima
muškog pola Wistar soja. Operativni zahvati i akutna registrovanja elektrofizioloških signala su obavljeni
pod Nembutal anestezijom. Registrovani su lokalni potencijali polja kore velikog i malog mozga u uslovima
pre i 1 h posle intraperitonealne (i.p.) administracije etarskog ulja kamforovog drveta ili 1,8-sineola.
Pacovima je intraperitonealno ubrizgavano etarsko ulje kamforovog drveta (150-750 μl/kg t.m., u 1 ml
fiziološkog rastvora i.p.) ili 1,8-sineol (300-500 μl/kg t.m., u 1 ml fiziološkog rastvora, i.p.). Digitalizovani
elektrokortikalni signali (frekvence semplovanja: 256 Hz) su analizirani uz pomoć brze Furijeove
transformacije, a potom je izračunavana vrednost fraktalne dimenzije (FD) i Hurstovog eksponenta (H).
Etarsko ulje kamforovog drveta (≥450 μl/kg t.m.) ili 1,8-si...neol (≥300 μl/kg t.m.) indukuju epileptičnu
aktivnost sa pojavom pojedinačnih i grupnih šiljaka velike amplitude u elektrokortikogramu sa povremenim
kloničnim konvulzijama ekstremiteta, 3-15 minuta nakon administracije supstanci. Na velikom mozgu,
nakon administracije etarskog ulja kamforovog drveta, kao i 1,8-sineola, u epohama sa epileptičnom
aktivnošću, bio je prisutan veliki porast ukupne snage kao i povećanje relativne snage u delta (0,1-4,0 Hz)
frekventnom opsegu, odnosno smanjenje relativne snage u teta (4,1-8,0 Hz), alfa (8,1-15,0 Hz), beta (15,1-
32,0 Hz) i gama (32,1-128,0 Hz) frekventnim opsezima. Potvrđeno je dominantno učešće delta (0,1-4,0 Hz)
frekventnog opsega a vrednost relativne snage se kretala od 47 do 87 %. Na malom mozgu, u epohama sa
epileptičnom aktivnošću, relativne snage frekventnih opsega su se različito ponašale u odnosu na veliki
mozak i različito nakon administracije 1,8-sineola u odnosu na etarsko ulje kamforovog drveta. Razlike
između velikog i malog mozga, kao i razlike u delovanju etarskog ulja kamforovog drveta i 1,8-sineola, bile
su izraženije u parametrima fraktalne analize. Na velikom mozgu pacova, nakon administracije etarskog ulja
kamforovog drveta, u epohama sa epileptičnom aktivnošću, postoji povećanje vrednosti FD u odnosu na
kontrolu, a na malom mozgu smanjenje. Suprotno, nakon administracije 1,8-sineola postoji smanjenje
vrednosti FD na velikom, odnosno povećanje na malom mozgu. Postojala je i dozna zavisnost odgovora
nakon administracije etarskog ulja kamforovog drveta, odnosno 1,8-sineola. Na velikom mozgu, povećanje
doze etarskog ulja kamforovog drveta, odnosno 1,8-sineola, u epohama sa epileptičnom aktivnošću, praćeno
je povećanjem relativne snage delta (0,1-4,0 Hz) frekventnog opsega, odnosno smanjenjem relativne snage
teta (4,1-8,0 Hz), alfa (8,1-15,0 Hz), beta (15,1-32,0 Hz) i gama (32,1-128,0 Hz) frekventnih opsega.
Suprotno, na malom mozgu, povećanje doze etarskog ulja kamforovog drveta u epohama sa epileptičnom
aktivnošću, praćeno je smanjenjem relativne snage delta (0,1-4,0 Hz), odnosno povećanjem relativne snage
teta (4,1-8,0 Hz), alfa (8,1-15,0 Hz) i beta (15,1-32,0 Hz) frekventnih opsega. Na malom mozgu, povećanje doze 1,8-sineola u epohama sa epileptičnom aktivnošću, praćeno je povećanjem relativne snage teta (4,1-8,0
Hz), odnosno smanjenjem relativne snage gama (32,1-128,0 Hz) frekventnog opsega. I na velikom i na
malom mozgu pacova, promene vrednosti FD negativno su korelisane sa povećanjem doze kako etarskog ulja
kamforovog drveta tako i 1,8-sineola...
We investigated the rat brain activity in acute seizures evoked by camphor tree essential oil or its main
constituent 1,8-cineole. The experiments were performed on adult male Wistar rats. The surgery and acute
electrophysiological recordings were performed under pentobarbital sodium anesthesia. Local field potentials
of cerebral and cerebellar parietal cortex were recorded before and 1 h after intraperitoneal (i.p.) camphor
essential oil or 1,8-cineole administration. The rats were intraperitoneally injected with camphor essential oil
(150-750 μl/kg b.w., in 1 ml of saline, i.p.) or 1,8-cineole (300-500 μl/kg b.w., in 1 ml of saline, i.p.). The
digitized electrocortical signals (sampling frequency: 256 Hz) were analyzed by the Fast Fourier Transform,
and were calculated Fractal dimension (FD) and Hurst exponent (H) also. Camphor essential oil (≥450 μl/kg
b.w.) and 1,8-cineole (≥300 μl/kg b.w.) induced seizure-like activity with single and multiple spiking of high
amplitudes in the par...ietal electrocorticogram and occasional clonic limb convulsions, 3-15 min after their
administration. On the cerebrum, after camphor essential oil and 1,8-cineole administration, in epochs with
epileptic activity, there was high increase in total power and an increase in relative power in delta (0,1-4,0
Hz) frequency range, as well as a decrease in relative power in theta (4,1-8,0 Hz), alpha (8,1-15,0 Hz), beta
(15,1-32,0 Hz) and gamma (32,1-128,0 Hz) frequency ranges. It was confirmed the dominant presence of the
delta (0,1- 4,0 Hz) frequency range and the values of the relative power ranged from 47 to 87 %. On the
cerebellum, in epochs with epileptic activity, the relative power of the frequency ranges were behaving
differently than cerebrum, as well as after administration of 1,8-cineole compared to the camphor essential
oil. The differences between the cerebrum and cerebellum, as well as differences in the activities of the
camphor essential oil and 1,8-cineole, were more pronounced in the parameters of fractal analysis. On the
cerebrum, after camphor essential oil aministration, in epochs with epileptic activity, there was an increase in
the FD value compared to the control, and the decrease on the cerebellum. In contrast, after 1,8-cineole
administration there was a decrease in FD value on the cerebrum, and increase on the cerebellum. There was
a dose dependence of response after the camphor essential oil and 1,8-cineole administration. On the
cerebrum, increasing the dose of the camphor essential oil and 1,8-cineole, in epochs with epileptic activity,
correlated with an increase in relative power in delta (0,1 to 4,0 Hz) frequency range and a decrease in
relative power in theta (4,1-8,0 Hz), alpha (8,1-15,0 Hz), beta (15,1-32,0 Hz) and gamma (32,1-128,0 Hz)
frequency ranges. In contrast, on the cerebellum, increasing the dose of the camphor essential oil in epochs
with epileptic activity, correlated with a decrease in relative power in delta (0,1 to 4,0 Hz) and increase in
relative power in theta (4,1 to 8,0 Hz), alpha (8,1 to 15,0 Hz) and beta (15,1 to 32,0 Hz) frequency ranges. On
the cerebellum, increasing the dose of 1,8-cineole, in epochs with epileptic activity, correlated with an
increase in relative power in theta (4,1-8,0 Hz) and a decrease in relative power in gamma (32,1-128,0 Hz) frequency range...
