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Biological activities of essential oils and extracts of selected Apiaceae species

dc.contributor.advisorMarin, Petar
dc.contributor.otherDžamić, Ana
dc.contributor.otherSoković, Marina
dc.contributor.otherRanđelović, Vladimir
dc.creatorMatejić, Jelena S.
dc.date.accessioned2016-01-05T11:47:38Z
dc.date.available2016-01-05T11:47:38Z
dc.date.available2020-07-03T08:09:47Z
dc.date.issued2013-05-18
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/2110
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=793
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:7117/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44652559
dc.description.abstractU ovom radu analizirana je biološka aktivnost etarskih ulja i ekstrakata sledećih vrsta familije Apiaceae: Eryngium serbicum Pančić, Seseli pallasii Besser, Seseli libanotis (L.) Koch subsp. libanotis, Seseli libanotis (L.) Koch subsp. intermedium (Rupr.) P. W. Ball, Peucedanum officinale L., Peucedanum longifolium W. et K., Peucedanum aegopodioides (Boiss.) Vand., Peucedanum alsaticum L., Pastinaca sativa L., Heracleum sphondylium L., Tordylium maximum L., Cachrys cristata DC. i Opopanax hispidus (Friv.) Griseb. Biljni materijal je ekstrahovan metanolom, acetonom i etil-acetatom, dok su vodeni ekstrakti liofilizirani. Etarska ulja navedenih vrsta i odabrani ekstrakti su testirani sa aspekta antimikrobne i antioksidativne aktivnosti. Kao pozitivne kontrole korišćeni su komercijalni antibiotici i antimikotici, a u testovima za utvrđivanje antioksidativne aktivnosti BHA i vitamin C. Antimikrobna aktivnost etarskih ulja i ekstrakata testirana je na: Gram-negativne bacterije (Escherichia coli, Pseudomons aeruginosa, Salmonella typhimurium, Salmonella enteritidis i Enterobacter cloacae) i Gram-pozitivne bacterije (Listeria monocytogenes, Bacillus cereus, Micrococcus flavus i Staphylococcus aureus), kao i na mikromicete (Aspergillus niger, A. ochraceus, A. versicolor, A. fumigatus, Penicillium funiculosum, P. ochrochloron, Trichoderma viride i Candida albicans). Kompozicija etarskih ulja je analizirana korišćenjem GC i GC/MS metoda. Metoda mikrodilucije na mikrotitracionim pločama je korišćena za testiranje antimikrobne aktivnosti. Određivane su minimalne inhibitorne i minimalne baktericidne/fungicidne koncentracije (MIC i MBC/MFC). DPPH i ABTS analize, kao i ukupan sadržaj fenola i flavonoida, su korišćene za određivanje antioksidativne aktivnosti. Etarsko ulje Eryngium serbicum je bilo najefikasniji antimikrobni agens, a slede ga ulja vrste roda Peucedanum, dok je etarsko ulje Seseli pallasii imalo najnižu antimikrobnu aktivnost. Acetonski i etil-acetatni ekstrakti su imali bolji antimikrobni potencijal od metanolnih i vodenih ekstrakata. Salmonella typhimurium je najosetljivijа bakterija, dok je Listeria monocytogenes, pokazala dobru otpornost na ispitivana ulja i ekstrakte. Rezultati testiranih gljiva pokazuju da je Aspergillus ochraceus bila najosetljivija, dok je Candida albicans bila najrezistentnija. Vodeni ekstrakti su bili najefikasniji u neutralizaciji DPPH radikala, dok su acetonski ekstrakti bili najefikasniji kod ABTS testa. Ekstrakti Peucedanum officinale su pokazali najbolju antioksidativnu aktivnost.sr
dc.description.abstractIn this work the biological activity of essential oils and extracts from following Apiaceae species was analyzed: Eryngium serbicum Pančić, Seseli pallasii Besser, Seseli libanotis (L.) Koch subsp. libanotis, Seseli libanotis (L.) Koch subsp. intermedium (Rupr.) P. W. Ball, Peucedanum officinale L., Peucedanum longifolium W. et K., Peucedanum aegopodioides (Boiss.) Vand., Peucedanum alsaticum L., Pastinaca sativa L., Heracleum sphondylium L., Tordylium maximum L., Cachrys cristata DC., Opopanax hispidus (Friv.) Griseb. Herbal samples, were extracted with methanol, acetone and ethil-acetate, while aqueous extracts were dried by freeze-dryer. Essential oils of above species, and selected extracts were evaluated for their antimicrobial and antioxidant activities. As a positive control commercial antibiotics and fungicides were used, while BHA and Vitamin C were used in antioxidant test. The antimicrobial activity of essential oils and extracts was investigated against: Gram (-) bacteria (Escherichia coli, Pseudomons aeruginosa, Salmonella typhimurium, Salmonella enteritidis, Enterobacter cloacae) and Gram (+) bacteria (Listeria monocytogenes, Bacillus cereus, Micrococcus flavus, Staphylococcus aureus), and micromycetes (Aspergillus niger, A. ochraceus, A. versicolor, A. fumigatus, Penicillium funiculosum, P. ochrochloron, Trichoderma viride, Candida albicans). The volatile constituents were analyzed using GC and GC/MS procedures. Microdilution method on microtitration plates was used for antimicrobial assay. Minimum inhibitory and minimum antibacterial/antifungal concentrations (MIC and MBC/MFC) were determined. DPPH and ABTS assay and total phenol and flavonoid content were used for antioxidant activity determination. Essential oil of Eryngium serbicum was the most effective antimicrobial agents, followed by Peucedanum species, while Seseli pallasii essential oil showed the lowest antimicrobial activity. Acetone and ethil-acetate extracts showed better antimicrobial potential than methanol and aquous extracts. Salmonella typhimurium were the most susceptible bacteria, while Listeria monocytogenes, showed good resistance to all of oils and extracts investigated. The results show that the tested micromycetes Aspergillus ochraceus was the most sensitive, while Candida albicans was the most resistant. Aquous extracts were the most effective DPPH radical scavangers, while acetone extracts were the most effective at ABTS test. Exstracts of Peucedanum officinale was with highest antioxidant activity.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Биолошки факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173029/RS//
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectApiaceaesr
dc.subjectApiaceaeen
dc.subjectetarska uljasr
dc.subjectekstraktisr
dc.subjectGC/MSsr
dc.subjectantimikrobna aktivnostsr
dc.subjectantioksidansisr
dc.subjectessential oilsen
dc.subjectextractsen
dc.subjectGC/MSen
dc.subjectantimicrobial activityen
dc.subjectantioxidantsen
dc.titleBiološka aktivnost etarskih ulja i ekstrakata odabranih vrsta iz familije Apiaceaesr
dc.titleBiological activities of essential oils and extracts of selected Apiaceae speciesen
dc.typedoctoralThesisen
dc.rights.licenseBY-NC
dcterms.abstractМарин, Петар; Ранђеловић, Владимир; Соковић, Марина; Джамић, Aна; Матејић, Јелена С.; Биолошка активност етарских уља и екстраката одабраних врста из фамилије Aпиацеае; Биолошка активност етарских уља и екстраката одабраних врста из фамилије Aпиацеае;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/2168/Disertacija.pdf
dc.identifier.doi10.2298/bg20130518matejic
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_2110


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