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Pomegranate peel extract (Punica granatum L.) as a natural antiviral agent for reduction of human norovirus in vitro

dc.contributor.advisorRadin, Dragoslava D.
dc.contributor.otherĐekić, Ivana
dc.contributor.otherBigović, Dubravka
dc.contributor.otherRadulović, Zorica
dc.contributor.otherPantić, Milena
dc.creatorŽivković, Ivana
dc.date.accessioned2022-12-08T20:35:59Z
dc.date.available2022-12-08T20:35:59Z
dc.date.issued2022-09-13
dc.identifier.urihttps://eteze.bg.ac.rs/application/showtheses?thesesId=8862
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:27129/bdef:Content/download
dc.identifier.urihttps://plus.cobiss.net/cobiss/sr/sr/bib/81856009
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/21041
dc.description.abstractHumani norovirusi (HuNoV) su dominatni uzročnici epidemija akutnog gastroenteritisa izazvanih kontaminiranom hranom. U cilju njihove redukcije sa površine svežeg povrća i površina koje dolaze u kontakt sa hranom, testirana je antinorovirusna aktivnost 11 ekstrakata lekovitih biljaka. Za dalja istraživanja izabran je ekstrakt kore nara, koji je u koncentraciji 5 mg/mL redukovao broj čestica HuNoV za 99% u fosfatnom puferu, simuliranim gastrointestinalnim fluidima i na površinama svežeg čeri paradajza i mladog luka, a za 91% u mleku. Matični sok od nara redukovao je HuNoV za 97%. Potencijal ekstrakta kore nara kao prirodnog dezinfekcionog sredstva na površinama od nerđajućeg čelika i plastike je značajan (> 3 log10 GE/μL) i u skladu sa predloženim normama koje se primenjuju za virusna dezinfekciona sredstva. Radi komparacije, testirano dezinfekciono sredstva na bazi hipohlorita (250 ppm) redukovalo je HuNoV na približno istom nivou. Proverena je dezinfekcija površina kontaminiranih mešavinom suspenzija HuNoV i koliformne bakterije Escherichia coli, primenom brzog ATP bioluminiscentnog testa i RT-qPCR metodom. Sredstvo na bazi hipohlorita bez naknadnog ispiranja, dalo je rezultat koji se našao u zoni upozorenja, dok su rezultati nakon ispiranja vodom detektovani u zoni čistog. Broj detektovanog HuNoV na nerđajućem čeliku i plastici, posle sanitacije sa sredstvom na bazi hipohlorita iznosio je 2,2 odnosno 2,38 log10 GE/μL. Takođe, pokušano je da se primenom alergen indikator protein testa detektuju različite koncentracije HuNoV (103- 106 GE/μL) nanete na prethodno sterilisanu površinu nerđajućeg čelika. Posle inkubacije na 70ºC/10 min, što povećava senzitivnost testa, najviša testirana koncentracija virusa dala je pozitivan rezultat.sr
dc.description.abstractHuman noroviruses (HuNoV) are the dominant agents of foodborne acute gastroenteritis and epidemic outbreaks. In order to reduce them from the surface of fresh vegetables and food-contact surfaces, the antinoroviral activity of 11 medicinal plant extracts was tested. For further research, pomegranate peel extract was selected, which at a concentration of 5 mg/mL reduced the number of HuNoV particles by 99% in phosphate buffer, simulated gastrointestinal fluids and on the surface of fresh cherry tomatoes and green onions, and by 91% in milk. Pomegranate juice reduced HuNoV by 97%. The potential of pomegranate peel extract as a natural disinfectant on stainless steel and plastic surfaces is significant (> 3 log10 GE/μL) and in accordance with the proposed standards applicable to viral disinfectants. For comparison, the tested hypochlorite-based disinfectant (250 ppm) reduced HuNoV to approximately the same level. Disinfection of surfaces contaminated with a mixture of suspension of HuNoV and coliform bacteria Escherichia coli was checked, using a rapid ATP bioluminescence test and RT-qPCR method. The hypochlorite-based agent without subsequent rinsing gave the result that was in the warning zone, while the results after rinsing with water were detected in the clean zone. The number of detected noroviruses on stainless steel and plastic, after sanitation with a hypochlorite-based agent was 2,2 and 2,38 log10 GE/μL, respectively. Also, an attempt was made to detect different concentrations of HuNoV (103-106 GE/μL) applied to a previously sterilized stainless steel surface using an allergen indicator protein test. After incubation at 70ºC/10 min which increases the sensitivity of the test, the highest tested virus concentration gave a positive result.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Пољопривредни факултетsr
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceУниверзитет у Београдуsr
dc.subjecthumani norovirusisr
dc.subjecthuman norovirusesen
dc.subjectmedicinal plant extractsen
dc.subjectpomegranate peel extracten
dc.subjectantinoroviral effecten
dc.subjectcommercial disinfectantsen
dc.subjectmedicinski biljni ekstraktisr
dc.subjectekstrakt kore narasr
dc.subjectantinorovirusni efekatsr
dc.subjectkomercijalni dezinficijensisr
dc.titleEkstrakt kore nara (Punica granatum L.) kao prirodni antivirusni agens za redukciju humanih norovirusa in vitrosr
dc.title.alternativePomegranate peel extract (Punica granatum L.) as a natural antiviral agent for reduction of human norovirus in vitroen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/148638/Disertacija_12965.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/152583/Referat.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_21041


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