Mitohondrijalna biogeneza kao mehanizam adaptacije specifičnih tipova ćelija reproduktivne osovine

Abstract

Sažetak: U potrazi za ulogom markera mitohondrijalne dinamike u adaptaciji ćelija reproduktivne osovine, sa posebnim fokusom na spermatozoide, dizajnirani su razliĉiti in vivo pristupi koji mimikriraju situacije vezane za poremećenu homeostazu cirkulišućih androgena (stres, hipogonadizam) u adultnoj humanoj populaciji. Rezultati pokazuju da ponavljani psihofiziĉki stres povećava transkripciju 82% ispitivanih markera mitohondrijalne dinamike i funkcionalnosti, kao i 73% transkripata za markere cAMP i MAPK signalinga koji regulišu homeostazu spermatozoida i mitohondrijalnu dinamiku/funkcionalnost u spermatozoidima. Oporavak organizma od akutnog psihofiziĉkog stresa dovodi do promene transkripcije 91% markera mitohondrijalne dinamike i funkcionalnosti, kao i 91% markera cAMP i MAPK signalinga. Ponavljani psihofiziĉki stres primenjen u razliĉitim vremenskim taĉkama u toku dana menja transkripciju 91% markera mitohondrijalne dinamike/funkcionalnosti, kao i 86% transkripata za elemente cAMP i MAPK signalinga. Hipogonadizam menja transkripciju 61% markera mitobiogeneze, fuzije/arhitekture i funkcionalnosti mitohondrija, kao i 59% transkripata za elemente cAMP i MAPK signalinga. Starenje u kombinaciji sa Sildenafilom menja ekspresiju 56% markera mitobiogeneze, fuzije/arhitekture i mitofunkcionalnosti, i ekspresiju 29% transkripata za elemente cAMP i MAPK signalinga. Starenje u kombinaciji sa Metforminom menja ekspresiju 75% markera mitobiogeneze, fuzije/arhitekture i mitofunkcionalnosti, i ekspresiju 77% markera cAMP i MAPK signalinga. Fiziološki znaĉaj se ogleda u promeni funkcionalnosti spermatozoida. Sumirano, promena ekspresije markera mitohondrijalne dinamike moţe biti jedan od adaptacionih mehanizma spermatozoida za oĉuvanje funkcionalnosti u uslovima poremećene homeostaze cirkulišućih androgena.

Abstract: In search of the role of mitochondrial dynamics markers in the adaptation of reproductive axis cells, with a special focus on spermatozoa, various in vivo approaches have been designed related to impaired circulating androgen homeostasis (stress, hypogonadism) that mimic situations in the adult human population. The results show that repeated psychophysical stress increases the transcription of 82% of the examined markers for the mitochondrial dynamics and functionality, as well as 73% of transcripts for markers of cAMP and MAPK signaling that regulate spermatozoa homeostasis and mitochondrial dynamics/functionality in spermatozoa. Recovery of the organism from acute psychophysical stress leads to a change in the transcription of 91% of mitochondrial dynamics and functionality markers, as well as 91% of markers of cAMP and MAPK signaling. Repeated psychophysical stress applied at different time points during the day alters 91% of transcripts of mitochondrial dynamics/functionality markers, as well as 86% of transcripts for cAMP and MAPK signaling elements. Hypogonadism alters the transcription of 61% of mitobiogenesis, fusion/architecture markers, and mitochondrial functionality markers, as well as 59% of transcripts for cAMP and MAPK signaling elements. Ageing in combination with Sildenafil alters the expression of 56% of mitobiogenesis, fusion/architecture and mitofunctionality markers, as well as the expression of 29% of transcripts for cAMP and MAPK signaling elements. Ageing in combination with Metformin alters the expression of 75% of mitobiogenesis, fusion/architecture, and mitofunctionality markers, and the expression of 77% of cAMP and MAPK signaling markers. Physiological significance is reflected in the changes in spermatozoa function. In summary, the change in the expression of mitochondrial dynamics markers may be one of the adaptive mechanisms of spermatozoa for the preservation of the functionality in the conditions of disturbed homeostasis of circulating androgens.