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Assessment of antimicrobial, anti-inflammatory, antioxidant and cardioprotective effects of wild garlic extract, Аllium ursinum L

dc.contributor.advisorTomović, Marina
dc.contributor.otherBradić, Jovana
dc.contributor.otherBolevič, Sergej
dc.contributor.otherJeremić, Jovana
dc.creatorKrivokapić, Miloš
dc.date.accessioned2021-10-28T09:34:43Z
dc.date.available2021-10-28T09:34:43Z
dc.date.issued2021-07-16
dc.identifier.urihttp://eteze.kg.ac.rs/application/showtheses?thesesId=8342
dc.identifier.urihttps://fedorakg.kg.ac.rs/fedora/get/o:1408/bdef:Content/download
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/18670
dc.description.abstractCilj ove studije bio je ispitivanje antimikrobnih, antioksidacionih, antiinflamacijskih i kardioprotektivnih efekata ekstrakata lista sremuša (Allium ursinum L). Za određivanje antimikrobnih efekata korišćen je mikrodilucioni test, dok je za ispitivanje ntiinflamacijskog potencijala korišćen model inflamacije izazvane karageninom na šapi pacova. Za ispitivanje antiinflamacijskog efekta uključeno je 50 pacova podeljenih u pet grupa: pacovi na tretmanu 0,9% NaCl, pacovi na tretmanu indometacinom i pacovi tretirani ekstraktom sremuša u tri doze-50, 100 i 200 mg/kg intraperitonealno (i.p.). Za ispitivanje kardioprotektivnih efekata ekstrakta sremuša korišćen je model retrogradne perfuzije po Langendorff-u. U ovaj deo eksperimenta uključeno je 80 pacova podeljenih u 8 grupa: zdravi i spontano hipertenzivni pacovi (SHR) koji su konzumirali ekstrakt sremuša u dozi od 125 mg/kg, 250 mg/kg i 500 mg/kg per os tokom 28 dana. U uzorcima koronarnog venskog efluenta određivani su nivoi prooksidanasa, dok je iz uzoraka venske krvi određivan sistemski redok status. Rezultati su pokazali generalno slabo antibakterijsko i antigljivično delovanje ekstrakata sremuša koje je bilo zavisno od vrste mikroorganizma i tipa ekstrakta. Metanolni ekstrakt sremuša u dozi od 200 mg/kg ispoljio je najintenzivniji efekat u redukciji inflamacije šape izazvane karageninom. Dodatno, unos ekstrakta sremuša tokom 28 dana značajno je doprineo oporavku srčane funkcije i ublažio oksidaciona oštećenja srca. Ekstrakt sremuša ispoljio je potencijal ka modulaciji sistemske redoks ravnoteže i istakao se kao snažan antioksidans. Benefiti ove biljne vrste uočeni su kako na modelu zdravih tako i kod SHR pacova pri čemu su izraženiji efekti bili prisutni nakon unosa više doze ekstrakta. Rezultati ove doktorske disertacije ukazuju na značajnu ulogu ekstrakta sremuša u kardioprotekciji i snažno antioksidaciono delovanje što ukazuje na mogućnost njegovog korišćenja samostalno u prevenciji ili kao adjuvantna antioksidaciona terapija kod kardiovaskularnih bolesti.sr
dc.description.abstracthe aim of this study was to investigate the antimicrobial, antioxidant, antiinflammatory and cardioprotective effects of Allium ursinum L extracts. A microdilution test was used to determine the antimicrobial effects, while a model of carrageenan-induced inflammation in rat paws was used to examine the anti-inflammatory potential. To examine the anti-inflammatory effect, 50 rats were divided into five groups: rats treated with 0.9% NaCl, rats treated with indomethacin and rats treated with wild garlic extract in three doses - 50, 100 and 200 mg / kg intraperitoneally (i.p.). A Langendorff retrograde perfusion model was used to examine the cardioprotective effects of wild garlic extract. This part of the experiment included 80 rats divided into 8 groups: healthy and spontaneously hypertensive rats (SHR) who consumed sremus extracts at a dose of 125 mg/kg, 250 mg/kg and 500 mg/kg per os for 28 days. Levels of pro-oxidants were determined in coronary venous effluent samples, while systemic redox status was determined in venous blood samples. Our results showed a generally weak antibacterial and antifungal effect of wild garlic extract, which was dependent on the type of microorganism and extract. Methanol extract of wild garlic at a dose of 200 mg/kg showed the most intensive effect in reducing paw inflammation caused by carrageenan. In addition, intake of wild garlic extract for 28 days significantly contributed to the recovery of cardiac function and alleviated oxidative damage to the heart. Wild garlic extract showed the potential to modulate the systemic redox balance and stood out as a powerful antioxidant. Benefits of this plant species were observed in both the model of healthy and SHR rats, with more pronounced effects observed after intake of a higher dose of the extract. The results of this doctoral thesis indicate a significant role of wild garlic extract in cardioprotection and strong antioxidant activity, which indicates the possibility of its use alone in prevention or as adjuvant antioxidant therapy in cardiovascular diseases.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Крагујевцу, Факултет медицинских наукаsr
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceУниверзитет у Крагујевцуsr
dc.subjectAllium ursinum, inflamacija, ishemijsko-reperfuziona povreda srca, oksidacioni stressr
dc.subjectAllium ursinum, inflammation, myocardial ischemia reperfusion injury, oxidative stressen
dc.titleIspitivanje antimikrobnih, antiinflamacijskih, antioksidacionih i kardioprotektivnih efekata ekstrakta sremuša, Allium ursinum Lsr
dc.title.alternativeAssessment of antimicrobial, anti-inflammatory, antioxidant and cardioprotective effects of wild garlic extract, Аllium ursinum Len
dc.typedoctoralThesis
dc.rights.licenseBY
dcterms.abstractТомовић, Марина; Брадић, Јована; Болевич, Сергеј; Јеремић, Јована; Кривокапић, Милош; Испитивање антимикробних, антиинфламацијских, антиоксидационих и кардиопротективних ефеката екстракта сремуша, Aллиум урсинум Л; Испитивање антимикробних, антиинфламацијских, антиоксидационих и кардиопротективних ефеката екстракта сремуша, Aллиум урсинум Л;
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/77133/Doctoral_thesis_11609.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/77338/bitstream_77338.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_18670


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