Primena biomimičnih bioreaktora u dizajniranju i karakterizaciji novih biomaterijala za inženjerstvo tkiva
Application of the biomimetic bioreactors in design and characterization of novel biomaterials for tissue engineering
Author
Zvicer, Jovana S.Mentor
Obradović, BojanaCommittee members
Janaćković, ĐorđeMišković-Stanković, Vesna
Stojkovska, Jasmina
Ignjatović, Nenad
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Show full item recordAbstract
Cilj istraživanja u ovoj doktorskoj disertaciji je bila primena biomimičnih bioreaktora za
sveobuhvatnu i fiziološki relevantnu karakterizaciju novih bioaktivnih biomaterijala za potencijalnu
primenu u inženjerstvu tkiva, a time i razvoj novih metodologija za pouzdanu evaluaciju
biomaterijala. Ispitani su nanokompozitni hidrogelovi alginata sa nanočesticama srebra za
inženjerstvo tkiva artikularne hrskavice i hidrogelovi gelanske gume sa nanočesticama bioaktivnog
stakla za inženjerstvo tkiva kosti i osteohondralnog tkiva primenom dva biomimična bioreaktora:
protočnog i bioreaktora sa dinamičkom kompresijom. Poseban cilj ove disertacije je bio i razvoj
novog bioreaktora za inženjerstvo tkiva intervertebralnog diska.
Ispitivanja nanokompozitnih Ag/alginatih hidrogelova baziranih na dve vrste alginata
pokazala su da polazni sastav alginata utiče na veličinu i kinetiku otpuštanja nanočestica srebra i
mehaničke karakteristike hidrogelova u fiziološki relevantnim bioreaktorskim uslovima, na ...osnovu
kojih je određena njihova potencijalna primena. Kinetika otpuštanja srebra je uspešno modelovana
prenosom mase difuzijom i konvekcijom.
Makroporozni hidrogelovi na bazi gelanske gume i bioaktivnog stakla sa otvorenim porama
su dobijeni unapređenom procedurom, a u fiziološki relevantnim uslovima u protočnom bioreaktoru
došlo je do značajnog formiranja kalcijum fosfata i poboljšanja mehaničkih karakteristika.
Razvijena je i jednostavna metoda za dobijanje dvofaznih osteohondralnih implantata sa dobrom
integracijom između slojeva.
Rezultati dobijeni u ovoj doktorskoj disertaciji su potvrdili značaj i široki potencijal primene
biomimičnih bioreaktora i sprovedene metodologije u više naučnih oblasti, od razvoja novih
biomaterijala i inženjerstva tkiva do nanotoksikologije i biologije ćelija.
The aim of this doctoral dissertation was application of biomimetic bioreactors for
comprehensive and physiologically relevant characterization of novel, bioactive biomaterials
intended for tissue engineering (TE), and, consequently development of new methodologies for
reliable biomaterial evaluation, as well. The focus was on nanocomposite alginate hydrogels with
silver nanoparticles (AgNPs) for articular cartilage TE and gellan gum hydrogels with bioactive
glass nanoparticles (GG-BAG) for bone and osteochondral TE, while two biomimetic bioreactors
were applied: perfusion and a bioreactor with dynamic compression. The specific goal of this
dissertation was also development of a novel bioreactor for intervertebral disc TE.
Investigation of nanocomposite Ag/alginate hydrogels based on two alginate types has
shown that the initial alginate composition affects the size and release kinetics of AgNPs, as well as
hydrogel mechanical properties under physiologically relevant bioreactor condit...ions, which
indicated the potential use for each alginate hydrogel type. The silver release kinetics was
successfully modeled by mass transfer by diffusion and advection-diffusion.
Macroporous GG-BAG hydrogels with open pores were obtained by a novel procedure.
Physiologically relevant conditions in perfusion bioreactors enhanced calcium phosphate formation
and improved hydrogel mechanical properties. Also, a simple method was developed for obtaining
biphasic osteochondral implants with good integration between layers.
The obtained results indicate the significance and vast potentials of biomimetic bioreactors
and methodologies developed in this doctoral dissertation for applications in different scientific
fields from biomaterials science and TE to nanotoxicology and cell biology.
Faculty:
Универзитет у Београду, Технолошко-металуршки факултетDate:
02-09-2020Projects:
- Synthesis, processing and applications of nanostructured multifunctional materials with defined properties (RS-45019)
- Synthesis, processing and applications of nanostructured multifunctional materials with defined properties (RS-45019)